Immobilization of gene vectors in perinuclear region as potential intracellular barrier to efficient gene delivery

J. Suh, J. Hanes

Research output: Contribution to conferencePaper

Abstract

Real time multiple tracking (MPT) was used to quantify the transport of gene vectors in live cells in order to study the dynamic process of intracellular gene delivery. The movement of fluorescently labeled polyethylenimine (PEI)/DNA nanocomplexes was captured with a camera attached to an epifluorescence microscope. USing MetaMorph software, vectors were tracked to obtain time-resolved positional data, which was used to calculate mean square displacements (MSD). Individual vector transport parameters, such as diffusivity and velocity, were obtained from the MSDs.

Original languageEnglish (US)
Pages8769-8770
Number of pages2
StatePublished - Dec 1 2004
Event2004 AIChE Annual Meeting - Austin, TX, United States
Duration: Nov 7 2004Nov 12 2004

Other

Other2004 AIChE Annual Meeting
CountryUnited States
CityAustin, TX
Period11/7/0411/12/04

ASJC Scopus subject areas

  • Engineering(all)

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  • Cite this

    Suh, J., & Hanes, J. (2004). Immobilization of gene vectors in perinuclear region as potential intracellular barrier to efficient gene delivery. 8769-8770. Paper presented at 2004 AIChE Annual Meeting, Austin, TX, United States.