Abstract
Real time multiple tracking (MPT) was used to quantify the transport of gene vectors in live cells in order to study the dynamic process of intracellular gene delivery. The movement of fluorescently labeled polyethylenimine (PEI)/DNA nanocomplexes was captured with a camera attached to an epifluorescence microscope. USing MetaMorph software, vectors were tracked to obtain time-resolved positional data, which was used to calculate mean square displacements (MSD). Individual vector transport parameters, such as diffusivity and velocity, were obtained from the MSDs.
| Original language | English (US) |
|---|---|
| Pages | 8769-8770 |
| Number of pages | 2 |
| State | Published - Dec 1 2004 |
| Event | 2004 AIChE Annual Meeting - Austin, TX, United States Duration: Nov 7 2004 → Nov 12 2004 |
Other
| Other | 2004 AIChE Annual Meeting |
|---|---|
| Country | United States |
| City | Austin, TX |
| Period | 11/7/04 → 11/12/04 |
ASJC Scopus subject areas
- Engineering(all)
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Cite this
Suh, J., & Hanes, J. (2004). Immobilization of gene vectors in perinuclear region as potential intracellular barrier to efficient gene delivery. 8769-8770. Paper presented at 2004 AIChE Annual Meeting, Austin, TX, United States.