Abstract
Real time multiple tracking (MPT) was used to quantify the transport of gene vectors in live cells in order to study the dynamic process of intracellular gene delivery. The movement of fluorescently labeled polyethylenimine (PEI)/DNA nanocomplexes was captured with a camera attached to an epifluorescence microscope. USing MetaMorph software, vectors were tracked to obtain time-resolved positional data, which was used to calculate mean square displacements (MSD). Individual vector transport parameters, such as diffusivity and velocity, were obtained from the MSDs.
| Original language | English (US) |
|---|---|
| Title of host publication | AIChE Annual Meeting, Conference Proceedings |
| Pages | 8769-8770 |
| Number of pages | 2 |
| State | Published - 2004 |
| Event | 2004 AIChE Annual Meeting - Austin, TX, United States Duration: Nov 7 2004 → Nov 12 2004 |
Other
| Other | 2004 AIChE Annual Meeting |
|---|---|
| Country | United States |
| City | Austin, TX |
| Period | 11/7/04 → 11/12/04 |
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ASJC Scopus subject areas
- Engineering(all)
Cite this
Immobilization of gene vectors in perinuclear region as potential intracellular barrier to efficient gene delivery. / Suh, J.; Hanes, Justin S.
AIChE Annual Meeting, Conference Proceedings. 2004. p. 8769-8770 504g.Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
}
TY - GEN
T1 - Immobilization of gene vectors in perinuclear region as potential intracellular barrier to efficient gene delivery
AU - Suh, J.
AU - Hanes, Justin S
PY - 2004
Y1 - 2004
N2 - Real time multiple tracking (MPT) was used to quantify the transport of gene vectors in live cells in order to study the dynamic process of intracellular gene delivery. The movement of fluorescently labeled polyethylenimine (PEI)/DNA nanocomplexes was captured with a camera attached to an epifluorescence microscope. USing MetaMorph software, vectors were tracked to obtain time-resolved positional data, which was used to calculate mean square displacements (MSD). Individual vector transport parameters, such as diffusivity and velocity, were obtained from the MSDs.
AB - Real time multiple tracking (MPT) was used to quantify the transport of gene vectors in live cells in order to study the dynamic process of intracellular gene delivery. The movement of fluorescently labeled polyethylenimine (PEI)/DNA nanocomplexes was captured with a camera attached to an epifluorescence microscope. USing MetaMorph software, vectors were tracked to obtain time-resolved positional data, which was used to calculate mean square displacements (MSD). Individual vector transport parameters, such as diffusivity and velocity, were obtained from the MSDs.
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UR - http://www.scopus.com/inward/citedby.url?scp=22544442754&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:22544442754
SP - 8769
EP - 8770
BT - AIChE Annual Meeting, Conference Proceedings
ER -