Immobilization of gene vectors in perinuclear region as potential intracellular barrier to efficient gene delivery

J. Suh; J. Hanes

Immobilization of gene vectors in perinuclear region as potential intracellular barrier to efficient gene delivery

School of Medicine

Research output: Contribution to conference › Paper › peer-review

Abstract

Real time multiple tracking (MPT) was used to quantify the transport of gene vectors in live cells in order to study the dynamic process of intracellular gene delivery. The movement of fluorescently labeled polyethylenimine (PEI)/DNA nanocomplexes was captured with a camera attached to an epifluorescence microscope. USing MetaMorph software, vectors were tracked to obtain time-resolved positional data, which was used to calculate mean square displacements (MSD). Individual vector transport parameters, such as diffusivity and velocity, were obtained from the MSDs.

Original language	English (US)
Pages	8769-8770
Number of pages	2
State	Published - Dec 1 2004
Event	2004 AIChE Annual Meeting - Austin, TX, United States Duration: Nov 7 2004 → Nov 12 2004

Other

Other	2004 AIChE Annual Meeting
Country/Territory	United States
City	Austin, TX
Period	11/7/04 → 11/12/04

ASJC Scopus subject areas

Engineering(all)

Cite this

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title = "Immobilization of gene vectors in perinuclear region as potential intracellular barrier to efficient gene delivery",

abstract = "Real time multiple tracking (MPT) was used to quantify the transport of gene vectors in live cells in order to study the dynamic process of intracellular gene delivery. The movement of fluorescently labeled polyethylenimine (PEI)/DNA nanocomplexes was captured with a camera attached to an epifluorescence microscope. USing MetaMorph software, vectors were tracked to obtain time-resolved positional data, which was used to calculate mean square displacements (MSD). Individual vector transport parameters, such as diffusivity and velocity, were obtained from the MSDs.",

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year = "2004",

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language = "English (US)",

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AU - Suh, J.

AU - Hanes, J.

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N2 - Real time multiple tracking (MPT) was used to quantify the transport of gene vectors in live cells in order to study the dynamic process of intracellular gene delivery. The movement of fluorescently labeled polyethylenimine (PEI)/DNA nanocomplexes was captured with a camera attached to an epifluorescence microscope. USing MetaMorph software, vectors were tracked to obtain time-resolved positional data, which was used to calculate mean square displacements (MSD). Individual vector transport parameters, such as diffusivity and velocity, were obtained from the MSDs.

AB - Real time multiple tracking (MPT) was used to quantify the transport of gene vectors in live cells in order to study the dynamic process of intracellular gene delivery. The movement of fluorescently labeled polyethylenimine (PEI)/DNA nanocomplexes was captured with a camera attached to an epifluorescence microscope. USing MetaMorph software, vectors were tracked to obtain time-resolved positional data, which was used to calculate mean square displacements (MSD). Individual vector transport parameters, such as diffusivity and velocity, were obtained from the MSDs.

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M3 - Paper

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T2 - 2004 AIChE Annual Meeting

Y2 - 7 November 2004 through 12 November 2004

ER -

Immobilization of gene vectors in perinuclear region as potential intracellular barrier to efficient gene delivery

Abstract

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ASJC Scopus subject areas

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