Immobilization of gene vectors in perinuclear region as potential intracellular barrier to efficient gene delivery

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Real time multiple tracking (MPT) was used to quantify the transport of gene vectors in live cells in order to study the dynamic process of intracellular gene delivery. The movement of fluorescently labeled polyethylenimine (PEI)/DNA nanocomplexes was captured with a camera attached to an epifluorescence microscope. USing MetaMorph software, vectors were tracked to obtain time-resolved positional data, which was used to calculate mean square displacements (MSD). Individual vector transport parameters, such as diffusivity and velocity, were obtained from the MSDs.

Original languageEnglish (US)
Title of host publicationAIChE Annual Meeting, Conference Proceedings
Pages8769-8770
Number of pages2
StatePublished - 2004
Event2004 AIChE Annual Meeting - Austin, TX, United States
Duration: Nov 7 2004Nov 12 2004

Other

Other2004 AIChE Annual Meeting
CountryUnited States
CityAustin, TX
Period11/7/0411/12/04

Fingerprint

Genes
Microscopes
DNA
Cameras

ASJC Scopus subject areas

  • Engineering(all)

Cite this

Suh, J., & Hanes, J. S. (2004). Immobilization of gene vectors in perinuclear region as potential intracellular barrier to efficient gene delivery. In AIChE Annual Meeting, Conference Proceedings (pp. 8769-8770). [504g]

Immobilization of gene vectors in perinuclear region as potential intracellular barrier to efficient gene delivery. / Suh, J.; Hanes, Justin S.

AIChE Annual Meeting, Conference Proceedings. 2004. p. 8769-8770 504g.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Suh, J & Hanes, JS 2004, Immobilization of gene vectors in perinuclear region as potential intracellular barrier to efficient gene delivery. in AIChE Annual Meeting, Conference Proceedings., 504g, pp. 8769-8770, 2004 AIChE Annual Meeting, Austin, TX, United States, 11/7/04.
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