Immediate and delayed (Late-phase) dermal contact sensitivity reactions in guinea pigs: Passive transfer by LgG1 antibodies, initiation by mast cell degranulation, and suppression by soybean proteinase inhibitor

K. Gregory Moore, Arthur M. Dannenbetg

Research output: Contribution to journalArticlepeer-review

Abstract

Specific immediate and delayed (24-hour) local dermal reactivity to oxazolone and dinitrochlorobenzene (DNCB) was passively transferred to naive guinea pigs by the intradermal injection of immune guinea pig serum and its IgGl fraction. In both actively sensitized and passively sensitized animals, neutrophils were the major cells present in the immediate reaction to the specific contactant. Basophils and mononuclear cells were the major cells present in the delayed reaction to the contactant. This late-phase reaction is, therefore, a cutaneous basophil hypersensitivity (CBH) response. The serum factor that passively transferred this CBH response was stable when heated at 56 °C for 1-4 h. Since transfer factor, cutaneous basophil hypersensitivity factor and guinea pig IgE are heat-sensitive, these factors probably made little or no contribution to this response. Because proteinase inhibitors are known to inhibit mast-cell degranulation in vitro, we tested the effect of soybean proteinase inhibitor in vivo. This inhibitor suppressed both the immediate and the delayed skin reactivities mediated by intradermal contactant-specific IgGl. These studies support the following concept: IgGl in guinea pigs (and IgE in human beings) sensitize mast cells to specific antigens. Such antigens degranulate mast cells, releasing histamine and other mediators for the immediate hypersensitivity reaction, and cause mast cells to produce cytokines that recruit basophils, eosinophils and mononuclear cells for the late-phase (CBH) reaction.

Original languageEnglish (US)
Pages (from-to)72-81
Number of pages10
JournalInternational archives of allergy and immunology
Volume101
Issue number1
DOIs
StatePublished - 1993

Keywords

  • Contact sensitivity
  • Cutaneous basophil hypersensitivity
  • Dinitrochlorobenzene
  • Hypersensitivity, immediate
  • IgGl antibody
  • Late-phase reactions
  • Mast cells, guinea pigs
  • Oxazolone
  • Proteinase inhibitor

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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