Imaging the evolution of reactivation pulmonary tuberculosis in mice using 18F-FDG PET

Allison M. Murawski, Saumya Gurbani, Jamie S. Harper, Mariah Klunk, Laurent Younes, Sanjay K. Jain, Bruno M. Jedynak

Research output: Contribution to journalArticle

Abstract

Methods: C3HeB/FeJ mice, which develop necrotic and hypoxic tuberculosis lesions, were aerosol-infected with Mycobacterium tuberculosis. PET and CT were used to serially image the same cohort of infected mice through pretreatment, tuberculosis treatment, and subsequent development of relapse.

Latent tuberculosis infection affects one third of the world's population and can reactivate (relapse) decades later. However, current technologies, dependent on postmortem analyses, cannot follow the temporal evolution of disease.

Results: A novel diffeomorphic registration was successfully used to monitor the spatial evolution of individual pulmonary lesions. Although most lesions during relapse developed in the same regions as those noted during pretreatment, several lesions also arose de novo within regions with no prior lesions.

Conclusion: This study presents a novel model that simulates infection and reactivation disease as seen in humans and could prove valuable to study tuberculosis pathogenesis and evaluate novel therapeutics.

Original languageEnglish (US)
Pages (from-to)1726-1729
Number of pages4
JournalJournal of Nuclear Medicine
Volume55
Issue number10
DOIs
StatePublished - Oct 1 2014

Keywords

  • Diffeomorphic registration
  • Latent
  • Mouse
  • Reactivation
  • Tuberculosis

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging

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    Murawski, A. M., Gurbani, S., Harper, J. S., Klunk, M., Younes, L., Jain, S. K., & Jedynak, B. M. (2014). Imaging the evolution of reactivation pulmonary tuberculosis in mice using 18F-FDG PET. Journal of Nuclear Medicine, 55(10), 1726-1729. https://doi.org/10.2967/jnumed.114.144634