TY - JOUR
T1 - Imaging prostate cancer invasion with multi-nuclear magnetic resonance methods
T2 - The metabolic boyden chamber
AU - Pilatus, Ulrich
AU - Ackerstaff, Ellen
AU - Artemov, Dmitri
AU - Mori, Noriko
AU - Gillies, Robert J.
AU - Bhujwalla, Zaver M.
N1 - Funding Information:
Address all correspondence to: Dr. Zaver M. Bhujwalla, PhD, Oncology Section, Division of MR Research, Department of Radiology, The Johns Hopkins University School of Medicine, Rm 208C Traylor Bldg., 720 Rutland Avenue, Baltimore, MD 21205. E-mail: zaver@mri.jhu.edu 1This work was supported by NIH grant 1R01 CA73850. 2Current address: ZRAD-Institut fur Neuroradiologie, Klinikum der Johann Wolfgang Goethe-Universitat, Frankfurt am Main, 60528 Frankfurt, Germany. Received 29 December 1999; Accepted 8 February 2000.
PY - 2000
Y1 - 2000
N2 - The physiological milieu within solid tumors can influence invasion and metastasis. To determine the impact of the physiological environment and cellular metabolism on cancer cell invasion, it is necessary to measure invasion during well-controlled modulation of the physiological environment. Recently, we demonstrated that magnetic resonance imaging can be used to monitor cancer cell invasion into a Matrigel layer [Artemov D, Pilatus U, Chou S, Mori N, Nelson JB, and Bhujwalla ZM (1999). Dynamics of prostate cancer cell invasion studied in vitro by NMR microscopy. Mag Res Med 42, 277- 282.]. Here we have developed an invasion assay ('Metabolic Boyden Chamber') that combines this capability with the properties of our isolated cell perfusion system. Long-term experiments can be performed to determine invasion as well as cellular metabolism-under controlled environmental conditions. To characterize the assay, we performed experiments with prostate cancer cell lines preselected for different invasive characteristics. The results showed invasion into, and degradation of the Matrigel layer, by the highly invasive/metastatic line (MatLyLu), whereas no significant changes were observed for the less invasive/metastatic cell line (DU-145). With this assay, invasion and metabolism was measured dynamically, together with oxygen tensions within the cellular environment and within the Matrigel layer. Such a system can be used to identify physiological and metabolic characteristics that promote invasion, and evaluate therapeutic interventions to inhibit invasion.
AB - The physiological milieu within solid tumors can influence invasion and metastasis. To determine the impact of the physiological environment and cellular metabolism on cancer cell invasion, it is necessary to measure invasion during well-controlled modulation of the physiological environment. Recently, we demonstrated that magnetic resonance imaging can be used to monitor cancer cell invasion into a Matrigel layer [Artemov D, Pilatus U, Chou S, Mori N, Nelson JB, and Bhujwalla ZM (1999). Dynamics of prostate cancer cell invasion studied in vitro by NMR microscopy. Mag Res Med 42, 277- 282.]. Here we have developed an invasion assay ('Metabolic Boyden Chamber') that combines this capability with the properties of our isolated cell perfusion system. Long-term experiments can be performed to determine invasion as well as cellular metabolism-under controlled environmental conditions. To characterize the assay, we performed experiments with prostate cancer cell lines preselected for different invasive characteristics. The results showed invasion into, and degradation of the Matrigel layer, by the highly invasive/metastatic line (MatLyLu), whereas no significant changes were observed for the less invasive/metastatic cell line (DU-145). With this assay, invasion and metabolism was measured dynamically, together with oxygen tensions within the cellular environment and within the Matrigel layer. Such a system can be used to identify physiological and metabolic characteristics that promote invasion, and evaluate therapeutic interventions to inhibit invasion.
KW - Invasion
KW - Magnetic resonance imaging
KW - Physiological environment
KW - Prostate cancer
KW - Spectroscopy
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U2 - 10.1038/sj.neo.7900089
DO - 10.1038/sj.neo.7900089
M3 - Article
C2 - 10935513
AN - SCOPUS:0034038709
SN - 1522-8002
VL - 2
SP - 273
EP - 279
JO - Neoplasia
JF - Neoplasia
IS - 3
ER -