Imaging pancreatic cancer using bioconjugated inp quantum dots

Ken Tye Yong, Hong Ding, Indrajit Roy, Wing Cheung Law, Earl J. Bergey, Anirban Maitra, Paras N. Prasad

Research output: Contribution to journalArticle

Abstract

In this paper, we report the successful use of non-cadmium-based quantum dots (QDs) as highly efficient and nontoxic optical probes for imaging live pancreatic cancer cells. Indium phosphide (core)-zinc sulfide (shell), or InP/ZnS, QDs with high quality and bright luminescence were prepared by a hot colloidal synthesis method in nonaqueous media. The surfaces of these QDs were then functionalized with mercaptosuccinic acid to make them highly dispersible in aqueous media. Further bioconjugation with pancreatic cancer specific monoclonal antibodies, such as anticlaudin 4 and antiprostate stem cell antigen (anti-PSCA), to the functionalized InP/ZnS QDs, allowed specific in vitro targeting of pancreatic cancer cell lines (both immortalized and low passage ones). The receptor-mediated delivery of the bioconjugates was further confirmed by the observation of poor in vitro targeting in nonpancreatic cancer based cell lines which are negative for the claudin-4-receptor. These observations suggest the immense potential of InP/ZnS QDs as non-cadmium-based safe and efficient optical imaging nanoprobes in diagnostic imaging, particularly for early detection of cancer.

Original languageEnglish (US)
Pages (from-to)502-510
Number of pages9
JournalACS Nano
Volume3
Issue number3
DOIs
StatePublished - Mar 24 2009

Fingerprint

Semiconductor quantum dots
cancer
quantum dots
Imaging techniques
Cells
cultured cells
Claudin-4
Nanoprobes
Indium phosphide
Zinc sulfide
indium phosphides
zinc sulfides
Monoclonal antibodies
stem cells
antigens
Antigens
Stem cells
antibodies
Luminescence
delivery

Keywords

  • Bioconjugates
  • Bioimaging
  • Pancreatic cancer
  • Quantum dots
  • Targeted delivery

ASJC Scopus subject areas

  • Engineering(all)
  • Materials Science(all)
  • Physics and Astronomy(all)

Cite this

Yong, K. T., Ding, H., Roy, I., Law, W. C., Bergey, E. J., Maitra, A., & Prasad, P. N. (2009). Imaging pancreatic cancer using bioconjugated inp quantum dots. ACS Nano, 3(3), 502-510. https://doi.org/10.1021/nn8008933

Imaging pancreatic cancer using bioconjugated inp quantum dots. / Yong, Ken Tye; Ding, Hong; Roy, Indrajit; Law, Wing Cheung; Bergey, Earl J.; Maitra, Anirban; Prasad, Paras N.

In: ACS Nano, Vol. 3, No. 3, 24.03.2009, p. 502-510.

Research output: Contribution to journalArticle

Yong, KT, Ding, H, Roy, I, Law, WC, Bergey, EJ, Maitra, A & Prasad, PN 2009, 'Imaging pancreatic cancer using bioconjugated inp quantum dots', ACS Nano, vol. 3, no. 3, pp. 502-510. https://doi.org/10.1021/nn8008933
Yong KT, Ding H, Roy I, Law WC, Bergey EJ, Maitra A et al. Imaging pancreatic cancer using bioconjugated inp quantum dots. ACS Nano. 2009 Mar 24;3(3):502-510. https://doi.org/10.1021/nn8008933
Yong, Ken Tye ; Ding, Hong ; Roy, Indrajit ; Law, Wing Cheung ; Bergey, Earl J. ; Maitra, Anirban ; Prasad, Paras N. / Imaging pancreatic cancer using bioconjugated inp quantum dots. In: ACS Nano. 2009 ; Vol. 3, No. 3. pp. 502-510.
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