Imaging of lipids in rat heart by MALDI-MS with silver nanoparticles

Shelley N. Jackson, Kathrine Baldwin, Ludovic Muller, Virginia M. Womack, J. Albert Schultz, Carey Balaban, Amina S. Woods

Research output: Contribution to journalArticle

Abstract

Lipids are a major component of heart tissue and perform several important functions such as energy storage, signaling, and as building blocks of biological membranes. The heart lipidome is quite diverse consisting of glycerophospholipids such as phosphatidylcholines (PCs), phosphatidylethanolamines (PEs), phosphatidylinositols (PIs), phosphatidylglycerols (PGs), cardiolipins (CLs), and glycerolipids, mainly triacylglycerols (TAGs). In this study, mass spectrometry imaging (MSI) enabled by matrix implantation of ionized silver nanoparticles (AgNP) was used to map several classes of lipids in heart tissue. The use of AgNP matrix implantation was motivated by our previous work showing that implantation doses of only 1014/cm2 of 2 nm gold nanoparticulates into the first 10 nm of the near surface of the tissue enabled detection of most brain lipids (including neutral lipid species such as cerebrosides) more efficiently than traditional organic MALDI matrices. Herein, a similar implantation of 500 eV AgNP- across the entire heart tissue section results in a quick, reproducible, solvent-free, uniform matrix concentration of 6 nm AgNP residing near the tissue surface. MALDI-MSI analysis of either positive or negative ions produce high-quality images of several heart lipid species. In negative ion mode, 24 lipid species [16 PEs, 4 PIs, 1 PG, 1 CL, 2 sphingomyelins (SMs)] were imaged. Positive ion images were also obtained from 29 lipid species (10 PCs, 5 PEs, 5 SMs, 9 TAGs) with the TAG species being heavily concentrated in vascular regions of the heart.

Original languageEnglish (US)
Pages (from-to)1377-1386
Number of pages10
JournalAnalytical and Bioanalytical Chemistry
Volume406
Issue number5
DOIs
StatePublished - Feb 2014
Externally publishedYes

Fingerprint

Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
Silver
Nanoparticles
Rats
Lipids
Imaging techniques
Phosphatidylethanolamines
Tissue
Phosphatidylglycerols
Triglycerides
Cardiolipins
Sphingomyelins
Ions
Phosphatidylinositols
Phosphatidylcholines
Mass spectrometry
Mass Spectrometry
Negative ions
Positive ions
Cerebrosides

Keywords

  • Lipids
  • MALDI
  • Mass spectrometry imaging
  • Silver nanoparticles

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry

Cite this

Jackson, S. N., Baldwin, K., Muller, L., Womack, V. M., Schultz, J. A., Balaban, C., & Woods, A. S. (2014). Imaging of lipids in rat heart by MALDI-MS with silver nanoparticles. Analytical and Bioanalytical Chemistry, 406(5), 1377-1386. https://doi.org/10.1007/s00216-013-7525-6

Imaging of lipids in rat heart by MALDI-MS with silver nanoparticles. / Jackson, Shelley N.; Baldwin, Kathrine; Muller, Ludovic; Womack, Virginia M.; Schultz, J. Albert; Balaban, Carey; Woods, Amina S.

In: Analytical and Bioanalytical Chemistry, Vol. 406, No. 5, 02.2014, p. 1377-1386.

Research output: Contribution to journalArticle

Jackson, SN, Baldwin, K, Muller, L, Womack, VM, Schultz, JA, Balaban, C & Woods, AS 2014, 'Imaging of lipids in rat heart by MALDI-MS with silver nanoparticles', Analytical and Bioanalytical Chemistry, vol. 406, no. 5, pp. 1377-1386. https://doi.org/10.1007/s00216-013-7525-6
Jackson SN, Baldwin K, Muller L, Womack VM, Schultz JA, Balaban C et al. Imaging of lipids in rat heart by MALDI-MS with silver nanoparticles. Analytical and Bioanalytical Chemistry. 2014 Feb;406(5):1377-1386. https://doi.org/10.1007/s00216-013-7525-6
Jackson, Shelley N. ; Baldwin, Kathrine ; Muller, Ludovic ; Womack, Virginia M. ; Schultz, J. Albert ; Balaban, Carey ; Woods, Amina S. / Imaging of lipids in rat heart by MALDI-MS with silver nanoparticles. In: Analytical and Bioanalytical Chemistry. 2014 ; Vol. 406, No. 5. pp. 1377-1386.
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