Imaging mass spectrometry in papillary thyroid carcinoma for the identification and validation of biomarker proteins

Kyueng Whan Min, Joo Young Bang, Kwang Pyo Kim, Wan Seop Kim, Sang Hwa Lee, Selina Rahman Shanta, Jeong Hwa Lee, Ji Hye Hong, So Dug Lim, Young Bum Yoo, Chan-Hyun Na

Research output: Contribution to journalArticle

Abstract

Direct tissue imaging mass spectrometry (IMS) by matrix-assisted laser desorption ionization and time-of-flight (MALDI-TOF) mass spectrometry has become increasingly important in biology and medicine, because this technology can detect the relative abundance and spatial distribution of interesting proteins in tissues. Five thyroid cancer samples, along with normal tissue, were sliced and transferred onto conductive glass slides. After laser scanning by MALDI-TOF equipped with a smart beam laser, images were created for individual masses and proteins were classified at 200-μm spatial resolution. Based on the spatial distribution, region-specific proteins on a tumor lesion could be identified by protein extraction from tumor tissue and analysis using liquid chromatography with tandem mass spectrometry (LC-MS/MS). Using all the spectral data at each spot, various intensities of a specific peak were detected in the tumor and normal regions of the thyroid. Differences in the molecular weights of expressed proteins between tumor and normal regions were analyzed using unsupervised and supervised clustering. To verify the presence of discovered proteins through IMS, we identified ribosomal protein P2, which is specific for cancer. We have demonstrated the feasibility of IMS as a useful tool for the analysis of tissue sections, and identified the tumor-specific protein ribosomal protein P2.

Original languageEnglish (US)
Pages (from-to)934-940
Number of pages7
JournalJournal of Korean Medical Science
Volume29
Issue number7
DOIs
StatePublished - 2014
Externally publishedYes

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Mass Spectrometry
Biomarkers
Proteins
Neoplasms
Lasers
Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
Tandem Mass Spectrometry
Papillary Thyroid cancer
Thyroid Neoplasms
Liquid Chromatography
Glass
Cluster Analysis
Thyroid Gland
Molecular Weight
Medicine
Technology

Keywords

  • Neoplasms
  • Pathology
  • Proteins
  • Spectrometry, mass, matrix-assisted laser desorption-ionization
  • Thyroid gland

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Imaging mass spectrometry in papillary thyroid carcinoma for the identification and validation of biomarker proteins. / Min, Kyueng Whan; Bang, Joo Young; Kim, Kwang Pyo; Kim, Wan Seop; Lee, Sang Hwa; Shanta, Selina Rahman; Lee, Jeong Hwa; Hong, Ji Hye; Lim, So Dug; Yoo, Young Bum; Na, Chan-Hyun.

In: Journal of Korean Medical Science, Vol. 29, No. 7, 2014, p. 934-940.

Research output: Contribution to journalArticle

Min, KW, Bang, JY, Kim, KP, Kim, WS, Lee, SH, Shanta, SR, Lee, JH, Hong, JH, Lim, SD, Yoo, YB & Na, C-H 2014, 'Imaging mass spectrometry in papillary thyroid carcinoma for the identification and validation of biomarker proteins', Journal of Korean Medical Science, vol. 29, no. 7, pp. 934-940. https://doi.org/10.3346/jkms.2014.29.7.934
Min, Kyueng Whan ; Bang, Joo Young ; Kim, Kwang Pyo ; Kim, Wan Seop ; Lee, Sang Hwa ; Shanta, Selina Rahman ; Lee, Jeong Hwa ; Hong, Ji Hye ; Lim, So Dug ; Yoo, Young Bum ; Na, Chan-Hyun. / Imaging mass spectrometry in papillary thyroid carcinoma for the identification and validation of biomarker proteins. In: Journal of Korean Medical Science. 2014 ; Vol. 29, No. 7. pp. 934-940.
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