IL-4 secretion and histamine release by human basophils are differentially regulated by protein kinase C activation

John T. Schroeder, Brian P. Howard, M. Kathleen Jenkens, Anne Kagey-Sobotka, Lawrence M. Lichtenstein, Donald W. MacGlashan

Research output: Contribution to journalArticlepeer-review

Abstract

The role of protein kinase C (PKC) activation was investigated in the secretion of interleukin-4 (IL-4) protein by human basophils. Phorbol myristate acetate (PMA) induced little to no detectable IL-4 protein in culture supernatants, despite being a potent secretagogue of histamine release by basophils. In fact, the secretion of IL-4 by basophils stimulated with ionomycin alone was down-regulated (30-70%) with the simultaneous addition of PMA. In peripheral blood lymphocytes (PBL), however, the combination of ionomycin and PMA were highly synergistic, resulting in maximum IL-4 release but at a slower rate. PKC inhibitors reversed these effects on IL-4 secretion. In sharp contrast to its inhibitory effect on IL- 4 protein secretion, PMA did not block the accumulation of IL-4 mRNA in basophils activated by ionomycin. These data suggest that there are marked differences in the regulatory processes for IL-4 transcription, translation, or secretion between basophils and lymphocytes.

Original languageEnglish (US)
Pages (from-to)692-698
Number of pages7
JournalJournal of Leukocyte Biology
Volume63
Issue number6
DOIs
StatePublished - Jun 1998

Keywords

  • Cytokine
  • IgE
  • Phorbol esters

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Cell Biology

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