TY - JOUR
T1 - IL-4 secretion and histamine release by human basophils are differentially regulated by protein kinase C activation
AU - Schroeder, John T.
AU - Howard, Brian P.
AU - Jenkens, M. Kathleen
AU - Kagey-Sobotka, Anne
AU - Lichtenstein, Lawrence M.
AU - MacGlashan, Donald W.
PY - 1998/6
Y1 - 1998/6
N2 - The role of protein kinase C (PKC) activation was investigated in the secretion of interleukin-4 (IL-4) protein by human basophils. Phorbol myristate acetate (PMA) induced little to no detectable IL-4 protein in culture supernatants, despite being a potent secretagogue of histamine release by basophils. In fact, the secretion of IL-4 by basophils stimulated with ionomycin alone was down-regulated (30-70%) with the simultaneous addition of PMA. In peripheral blood lymphocytes (PBL), however, the combination of ionomycin and PMA were highly synergistic, resulting in maximum IL-4 release but at a slower rate. PKC inhibitors reversed these effects on IL-4 secretion. In sharp contrast to its inhibitory effect on IL- 4 protein secretion, PMA did not block the accumulation of IL-4 mRNA in basophils activated by ionomycin. These data suggest that there are marked differences in the regulatory processes for IL-4 transcription, translation, or secretion between basophils and lymphocytes.
AB - The role of protein kinase C (PKC) activation was investigated in the secretion of interleukin-4 (IL-4) protein by human basophils. Phorbol myristate acetate (PMA) induced little to no detectable IL-4 protein in culture supernatants, despite being a potent secretagogue of histamine release by basophils. In fact, the secretion of IL-4 by basophils stimulated with ionomycin alone was down-regulated (30-70%) with the simultaneous addition of PMA. In peripheral blood lymphocytes (PBL), however, the combination of ionomycin and PMA were highly synergistic, resulting in maximum IL-4 release but at a slower rate. PKC inhibitors reversed these effects on IL-4 secretion. In sharp contrast to its inhibitory effect on IL- 4 protein secretion, PMA did not block the accumulation of IL-4 mRNA in basophils activated by ionomycin. These data suggest that there are marked differences in the regulatory processes for IL-4 transcription, translation, or secretion between basophils and lymphocytes.
KW - Cytokine
KW - IgE
KW - Phorbol esters
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U2 - 10.1002/jlb.63.6.692
DO - 10.1002/jlb.63.6.692
M3 - Article
C2 - 9620661
AN - SCOPUS:0031801825
SN - 0741-5400
VL - 63
SP - 692
EP - 698
JO - Journal of Leukocyte Biology
JF - Journal of Leukocyte Biology
IS - 6
ER -