IL-13 selectively induces vascular cell adhesion molecule-1 expression in human endothelial cells

B. S. Bochner, D. A. Klunk, S. A. Sterbinsky, R. L. Coffman, R. P. Schleimer

Research output: Contribution to journalArticle

Abstract

Previous studies with human umbilical vein endothelial cells (HUVEC) have shown that the cytokine IL-4 induces adherence of human eosinophils, but not neutrophils, because of its ability to selectively induce surface expression of vascular cell adhesion molecule-1 (VCAM-1). Because the cytokine IL-13 shares a number of biologic properties with IL-4, we examined the effect of IL-13 on the expression and function of adhesion molecules on HUVEC. Incubation of HUVEC for 4 to 48 h with IL-13 (0.1 to 15 U/ml) induced surface expression of VCAM-1, as detected by indirect immunofluorescence and flow cytometry, without significantly affecting expression of E-selectin or intercellular adhesion molecule-1. The kinetics and maximal IL-13-induced expression of VCAM-1 were similar to those seen with IL-4. Treatment of HUVEC with an optimal concentration of IL-13 (15 U/ml for 24 h) induced adhesiveness for eosinophils, but not for neutrophils, and adhesion was completely inhibited by mAb recognizing VCAM-1 or α4 integrin (CD49d). These results demonstrate that IL-13, like IL-4, selectively stimulates HUVEC to express functional cell surface VCAM-1 and suggest a possible role for IL-13 in promoting VCAM-1/α4 integrin-dependent accumulation of eosinophils during allergic and other inflammatory reactions in vivo.

Original languageEnglish (US)
Pages (from-to)799-803
Number of pages5
JournalJournal of Immunology
Volume154
Issue number2
StatePublished - 1995

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Interleukin-13
Vascular Cell Adhesion Molecule-1
Human Umbilical Vein Endothelial Cells
Endothelial Cells
Interleukin-4
Eosinophils
Integrins
Neutrophils
Cytokines
Interleukin-15
Adhesiveness
E-Selectin
Intercellular Adhesion Molecule-1
Indirect Fluorescent Antibody Technique
Flow Cytometry

ASJC Scopus subject areas

  • Immunology

Cite this

Bochner, B. S., Klunk, D. A., Sterbinsky, S. A., Coffman, R. L., & Schleimer, R. P. (1995). IL-13 selectively induces vascular cell adhesion molecule-1 expression in human endothelial cells. Journal of Immunology, 154(2), 799-803.

IL-13 selectively induces vascular cell adhesion molecule-1 expression in human endothelial cells. / Bochner, B. S.; Klunk, D. A.; Sterbinsky, S. A.; Coffman, R. L.; Schleimer, R. P.

In: Journal of Immunology, Vol. 154, No. 2, 1995, p. 799-803.

Research output: Contribution to journalArticle

Bochner, BS, Klunk, DA, Sterbinsky, SA, Coffman, RL & Schleimer, RP 1995, 'IL-13 selectively induces vascular cell adhesion molecule-1 expression in human endothelial cells', Journal of Immunology, vol. 154, no. 2, pp. 799-803.
Bochner BS, Klunk DA, Sterbinsky SA, Coffman RL, Schleimer RP. IL-13 selectively induces vascular cell adhesion molecule-1 expression in human endothelial cells. Journal of Immunology. 1995;154(2):799-803.
Bochner, B. S. ; Klunk, D. A. ; Sterbinsky, S. A. ; Coffman, R. L. ; Schleimer, R. P. / IL-13 selectively induces vascular cell adhesion molecule-1 expression in human endothelial cells. In: Journal of Immunology. 1995 ; Vol. 154, No. 2. pp. 799-803.
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