IL-13 released by and localized in human basophils

Huamin Li, Tommy C. Sim, Rafeul Alam

Research output: Contribution to journalArticle

Abstract

We and others have shown that human basophils can synthesize and release IL-4. However, IL-13, a cytokine that closely resembles IL-4, has not hitherto been described as a basophil product. The production of IL-13 by basophils was demonstrated by immunocytochemistry. Approximately 70% of basophils stimulated with anti-FcεR1α (antibody to the α subunit of IgE receptor type 1) stained for IL-13. Under similar experimental conditions, mononuclear cells failed to stain for IL-13. The cytokine was localized to basophilic granules by electron microscopic examination of immunogold staining. The secretion of IL-13 into the culture supernatant was assayed by ELISA. Kinetic studies showed detectable IL-13 release at 3 h, which steadily increased up to 24 h. This is significantly different from the kinetics of basophil histamine and IL-4 release. IL-13 production was also observed upon stimulation with anti-IgE, anti-FcεRIα, IL-3, and A23187 in a dose- dependent manner. PBMC, neutrophils, and eosinophils isolated from the same donors did not release IL-13 after anti-IgE stimulation. The anti-IgE- induced basophil IL-13 synthesis could be enhanced by IL-3 preincubation (with and without IL-3 preincubation, anti-IgE-induced IL-13 production was 227 ± 99 and 42 ± 13 pg/106 basophils, respectively). PBMC produced a significant amount of IL-13 upon stimulation with PHA, but a low level of IL- 13 in response to A23187 and/or PMA. Eosinophils and neutrophils did not produce IL-13 when cultured with A23187, IL-5, and anti-FcεRIα. This is the first demonstration of IL-13 production by basophils. Our data suggest that basophils, in addition to secreting mediators, can represent an important source of proallergic cytokines.

Original languageEnglish (US)
Pages (from-to)4833-4838
Number of pages6
JournalJournal of Immunology
Volume156
Issue number12
StatePublished - Jun 15 1996
Externally publishedYes

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Interleukin-13
Basophils
Interleukin-3
Calcimycin
Interleukin-4
Cytokines
Eosinophils
Neutrophils
IgE Receptors
Interleukin-5
Histamine
Anti-Idiotypic Antibodies

ASJC Scopus subject areas

  • Immunology

Cite this

Li, H., Sim, T. C., & Alam, R. (1996). IL-13 released by and localized in human basophils. Journal of Immunology, 156(12), 4833-4838.

IL-13 released by and localized in human basophils. / Li, Huamin; Sim, Tommy C.; Alam, Rafeul.

In: Journal of Immunology, Vol. 156, No. 12, 15.06.1996, p. 4833-4838.

Research output: Contribution to journalArticle

Li, H, Sim, TC & Alam, R 1996, 'IL-13 released by and localized in human basophils', Journal of Immunology, vol. 156, no. 12, pp. 4833-4838.
Li H, Sim TC, Alam R. IL-13 released by and localized in human basophils. Journal of Immunology. 1996 Jun 15;156(12):4833-4838.
Li, Huamin ; Sim, Tommy C. ; Alam, Rafeul. / IL-13 released by and localized in human basophils. In: Journal of Immunology. 1996 ; Vol. 156, No. 12. pp. 4833-4838.
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