IL-13 induces YY1 through the AKT pathway in lung fibroblasts

Jia Guo, Hongwei Yao, Xin Lin, Haodong Xu, David Dean, Zhou Zhu, Gang Liu, Patricia Sime

Research output: Contribution to journalArticle

Abstract

A key feature of lung fibrosis is the accumulation of myofibroblasts. Interleukin 13 (IL-13) is a pro-fibrotic mediator that directly and indirectly influences the activation of myofibroblasts. Transforming growth factor beta (TGF-β) promotes the differentiation of fibroblasts into myofibroblasts, and can be regulated by IL-13. However, IL-13's downstream signaling pathways are not completely understood.We previously reported that the transcription factor Yin Yang 1 (YY1) is upregulated in fibroblasts treated with TGF-β and in the lungs of mice and patients with pulmonary fibrosis. Moreover, YY1 directly regulates collagen and alpha smooth muscle actin (a-SMA) expression in fibroblasts. However, it is not known if IL-13 regulates fibroblast activation through YY1 expression. We hypothesize that IL-13 upregulates YY1 expression through regulation of AKT activation, leading to fibroblast activation. In this study we found that YY1 was upregulated by IL-13 in lung fibroblasts in a doseand time-dependent manner, resulting in increased a-SMA. Conversely, knockdown of YY1 blocked IL-13-induced a-SMA expression in fibroblasts. Furthermore, AKT phosphorylation was increased in fibroblasts treated with IL-13, and AKT overexpression upregulated YY1, whereas blockade of AKT phosphorylation suppressed the induction of YY1 by IL-13 in vitro. In vivo YY1 was upregulated in fibrotic lungs from CC10-IL-13 transgenic mice compared to that from wild-type littermates, which was associated with increased AKT phosphorylation. Taken together, these findings demonstrate that IL-13 is a potent stimulator and activator of fibroblasts, at least in part, through AKT-mediated YY1 activation.

Original languageEnglish (US)
Article numbere0119039
JournalPLoS One
Volume10
Issue number3
DOIs
StatePublished - Mar 16 2015
Externally publishedYes

Fingerprint

Yin-Yang
Interleukin-13
Fibroblasts
fibroblasts
lungs
Lung
Chemical activation
Phosphorylation
Myofibroblasts
smooth muscle
actin
Smooth Muscle
Muscle
Actins
phosphorylation
transforming growth factor beta
fibrosis
Transforming Growth Factor beta
interleukin-13
Pulmonary Fibrosis

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Guo, J., Yao, H., Lin, X., Xu, H., Dean, D., Zhu, Z., ... Sime, P. (2015). IL-13 induces YY1 through the AKT pathway in lung fibroblasts. PLoS One, 10(3), [e0119039]. https://doi.org/10.1371/journal.pone.0119039

IL-13 induces YY1 through the AKT pathway in lung fibroblasts. / Guo, Jia; Yao, Hongwei; Lin, Xin; Xu, Haodong; Dean, David; Zhu, Zhou; Liu, Gang; Sime, Patricia.

In: PLoS One, Vol. 10, No. 3, e0119039, 16.03.2015.

Research output: Contribution to journalArticle

Guo, J, Yao, H, Lin, X, Xu, H, Dean, D, Zhu, Z, Liu, G & Sime, P 2015, 'IL-13 induces YY1 through the AKT pathway in lung fibroblasts', PLoS One, vol. 10, no. 3, e0119039. https://doi.org/10.1371/journal.pone.0119039
Guo J, Yao H, Lin X, Xu H, Dean D, Zhu Z et al. IL-13 induces YY1 through the AKT pathway in lung fibroblasts. PLoS One. 2015 Mar 16;10(3). e0119039. https://doi.org/10.1371/journal.pone.0119039
Guo, Jia ; Yao, Hongwei ; Lin, Xin ; Xu, Haodong ; Dean, David ; Zhu, Zhou ; Liu, Gang ; Sime, Patricia. / IL-13 induces YY1 through the AKT pathway in lung fibroblasts. In: PLoS One. 2015 ; Vol. 10, No. 3.
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