IL-13-induced chemokine responses in the lung

Role of CCR2 in the pathogenesis of IL-13-induced inflammation and remodeling

Zhou Zhu, Bing Ma, Tao Zheng, Robert J. Homer, Chun Geun Lee, Israel F. Charo, Paul Noble, Jack A. Elias

Research output: Contribution to journalArticle

Abstract

IL-13 stimulates inflammatory and remodeling responses and contributes to the pathogenesis of human airways disorders. To further understand the cellular and molecular events that mediate these responses, we characterized the effects of IL-13 on monocyte chemotactic proteins (MCPs) and compared the tissue effects of transgenic IL-13 in mice with wild-type (+/+) and null (-/-) CCR2 loci. Transgenic IL-13 was a potent stimulator of MCP-1, -2, -3, and -5. This stimulation was not specific for MCPs because macrophage-inflammatory protein (MIP)-1α, MIP-1β, MIP-2, MIP-3α, thymus- and activation-regulated chemokine, thymus-expressed chemokine, eotaxin, eotaxin 2, macrophage-derived chemokines, and C10 were also induced. The ability of IL-13 to increase lung size, alveolar size, and lung compliance, to stimulate pulmonary inflammation, hyaluronic acid accumulation, and tissue fibrosis, and to cause respiratory failure and death were markedly decreased, whereas mucus metaplasia was not altered in CCR2-/- mice. CCR2 deficiency did not decrease the basal or IL-13-stimulated expression of target matrix metalloproteinases or cathepsins but did increase the levels of mRNA encoding α1-antitrypsin, tissue inhibitor of metalloproteinase-1, -2, and -4, and secretory leukocyte proteinase inhibitor. In addition, the levels of bioactive and total TGF-β1 were decreased in lavage fluids from IL-13 transgenic mice with -/- CCR2 loci. These studies demonstrate that IL-13 is a potent stimulator of MCPs and other CC chemokines and document the importance of MCP-CCR2 signaling in the pathogenesis of the IL-13-induced pulmonary phenotype.

Original languageEnglish (US)
Pages (from-to)2953-2962
Number of pages10
JournalJournal of Immunology
Volume168
Issue number6
StatePublished - Mar 15 2002
Externally publishedYes

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Interleukin-13
Chemokines
Monocyte Chemoattractant Proteins
Inflammation
Lung
Macrophage Inflammatory Proteins
Chemokine CCL8
Chemokine CCL24
Chemokine CCL22
Chemokine CCL17
Chemokine CXCL2
Lung Compliance
Cathepsins
Tissue Inhibitor of Metalloproteinase-2
CC Chemokines
Tissue Inhibitor of Metalloproteinase-1
Chemokine CCL2
Therapeutic Irrigation
Metaplasia
Hyaluronic Acid

ASJC Scopus subject areas

  • Immunology

Cite this

Zhu, Z., Ma, B., Zheng, T., Homer, R. J., Lee, C. G., Charo, I. F., ... Elias, J. A. (2002). IL-13-induced chemokine responses in the lung: Role of CCR2 in the pathogenesis of IL-13-induced inflammation and remodeling. Journal of Immunology, 168(6), 2953-2962.

IL-13-induced chemokine responses in the lung : Role of CCR2 in the pathogenesis of IL-13-induced inflammation and remodeling. / Zhu, Zhou; Ma, Bing; Zheng, Tao; Homer, Robert J.; Lee, Chun Geun; Charo, Israel F.; Noble, Paul; Elias, Jack A.

In: Journal of Immunology, Vol. 168, No. 6, 15.03.2002, p. 2953-2962.

Research output: Contribution to journalArticle

Zhu, Z, Ma, B, Zheng, T, Homer, RJ, Lee, CG, Charo, IF, Noble, P & Elias, JA 2002, 'IL-13-induced chemokine responses in the lung: Role of CCR2 in the pathogenesis of IL-13-induced inflammation and remodeling', Journal of Immunology, vol. 168, no. 6, pp. 2953-2962.
Zhu, Zhou ; Ma, Bing ; Zheng, Tao ; Homer, Robert J. ; Lee, Chun Geun ; Charo, Israel F. ; Noble, Paul ; Elias, Jack A. / IL-13-induced chemokine responses in the lung : Role of CCR2 in the pathogenesis of IL-13-induced inflammation and remodeling. In: Journal of Immunology. 2002 ; Vol. 168, No. 6. pp. 2953-2962.
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