IL-11 expression is increased in severe asthma: Association with epithelial cells and eosinophils

Eleanor Minshall, Jamila Chakir, Michel Laviolette, Sophie Molet, Zhou Zhu, Ron Olivenstein, Jack A. Elias, Qutayba Hamid

Research output: Contribution to journalArticle

Abstract

Background: IL-11 is a pleiotropic cytokine produced by a variety of stromal cells. Targeted overexpression of this cytokine in mice results in a remodeling of the airways and the development of airway hyperresponsiveness and airway obstruction. Objectives: Because these alterations mimic important pathologic and physiologic changes in the airways of some asthmatic patients, we investigated the expression of IL-11 messenger RNA (mRNA) within the airways of patients with mild to severe asthma and nonasthmatic control subjects. Methods: Fiberoptic bronchoscopy to obtain bronchial biopsy specimens was performed on patients with mild (n = 13), moderate (n = 10), and severe (n = 9) asthma and on nonasthmatic control subjects (n = 9). Results: These patients differed in their extent of airway fibrosis with types I and III collagens being noted in greater quantities in the biopsy specimens from the severe and moderate asthmatics than in those from controls (P < .05). IL-11 mRNA expression was observed in the epithelial and subepithelial layers of asthmatic and nonasthmatic control subjects. The number of cells within the epithelium and subepithelium expressing IL-11 mRNA was greater in those with moderate and severe asthma compared with mild asthma and nonasthmatic subjects (P < .001). There were also greater numbers of IL-11 mRNA-positive cells within the subepithelium in severe compared with moderate asthma (P < .001). Immunostaining for IL-11 within the airway tissues confirmed translation of the mRNA into IL-11-immunoreactive protein in airway epithelial cells. Colocalization of IL-11 mRNA and immunoreactivity with resident inflammatory cells demonstrated that this cytokine was also expressed by major basic protein-positive eosinophils. Conclusion: These results suggest that IL-11 is involved in the chronic remodeling seen in asthmatic airways and is associated with increasing severity of the disease.

Original languageEnglish (US)
Pages (from-to)232-238
Number of pages7
JournalJournal of Allergy and Clinical Immunology
Volume105
Issue number2 II
StatePublished - Dec 1 2000

Keywords

  • Airway remodeling
  • Airways inflammation
  • Collagen deposition
  • IL-11
  • Severe asthma

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Fingerprint Dive into the research topics of 'IL-11 expression is increased in severe asthma: Association with epithelial cells and eosinophils'. Together they form a unique fingerprint.

  • Cite this

    Minshall, E., Chakir, J., Laviolette, M., Molet, S., Zhu, Z., Olivenstein, R., Elias, J. A., & Hamid, Q. (2000). IL-11 expression is increased in severe asthma: Association with epithelial cells and eosinophils. Journal of Allergy and Clinical Immunology, 105(2 II), 232-238.