IL-11 expression is increased in severe asthma: Association with epithelial cells and eosinophils

Eleanor Minshall, Jamila Chakir, Michel Laviolette, Sophie Molet, Zhou Zhu, Ron Olivenstein, Jack A. Elias, Qutayba Hamid

Research output: Contribution to journalArticle

Abstract

Background: IL-11 is a pleiotropic cytokine produced by a variety of stromal cells. Targeted overexpression of this cytokine in mice results in a remodeling of the airways and the development of airway hyperresponsiveness and airway obstruction. Objectives: Because these alterations mimic important pathologic and physiologic changes in the airways of some asthmatic patients, we investigated the expression of IL-11 messenger RNA (mRNA) within the airways of patients with mild to severe asthma and nonasthmatic control subjects. Methods: Fiberoptic bronchoscopy to obtain bronchial biopsy specimens was performed on patients with mild (n = 13), moderate (n = 10), and severe (n = 9) asthma and on nonasthmatic control subjects (n = 9). Results: These patients differed in their extent of airway fibrosis with types I and III collagens being noted in greater quantities in the biopsy specimens from the severe and moderate asthmatics than in those from controls (P <.05). IL-11 mRNA expression was observed in the epithelial and subepithelial layers of asthmatic and nonasthmatic control subjects. The number of cells within the epithelium and subepithelium expressing IL-11 mRNA was greater in those with moderate and severe asthma compared with mild asthma and nonasthmatic subjects (P <.001). There were also greater numbers of IL-11 mRNA-positive cells within the subepithelium in severe compared with moderate asthma (P <.001). Immunostaining for IL-11 within the airway tissues confirmed translation of the mRNA into IL-11-immunoreactive protein in airway epithelial cells. Colocalization of IL-11 mRNA and immunoreactivity with resident inflammatory cells demonstrated that this cytokine was also expressed by major basic protein-positiVe eosinophils. Conclusion: These results suggest that IL-11 is involved in the chronic remodeling seen in asthmatic airways and is associated with increasing severity of the disease.

Original languageEnglish (US)
Pages (from-to)232-238
Number of pages7
JournalThe Journal of Allergy and Clinical Immunology
Volume105
Issue number2 I
StatePublished - 2000
Externally publishedYes

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Interleukin-11
Eosinophils
Asthma
Epithelial Cells
Messenger RNA
Cytokines
Eosinophil Major Basic Protein
Airway Remodeling
Biopsy
Collagen Type III
Bronchoscopy
Airway Obstruction
Stromal Cells
Collagen Type I
Fibrosis
Epithelium
Cell Count

Keywords

  • Airway remodeling
  • Airways inflammation
  • Collagen deposition
  • IL-11
  • Severe asthma

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Minshall, E., Chakir, J., Laviolette, M., Molet, S., Zhu, Z., Olivenstein, R., ... Hamid, Q. (2000). IL-11 expression is increased in severe asthma: Association with epithelial cells and eosinophils. The Journal of Allergy and Clinical Immunology, 105(2 I), 232-238.

IL-11 expression is increased in severe asthma : Association with epithelial cells and eosinophils. / Minshall, Eleanor; Chakir, Jamila; Laviolette, Michel; Molet, Sophie; Zhu, Zhou; Olivenstein, Ron; Elias, Jack A.; Hamid, Qutayba.

In: The Journal of Allergy and Clinical Immunology, Vol. 105, No. 2 I, 2000, p. 232-238.

Research output: Contribution to journalArticle

Minshall, E, Chakir, J, Laviolette, M, Molet, S, Zhu, Z, Olivenstein, R, Elias, JA & Hamid, Q 2000, 'IL-11 expression is increased in severe asthma: Association with epithelial cells and eosinophils', The Journal of Allergy and Clinical Immunology, vol. 105, no. 2 I, pp. 232-238.
Minshall E, Chakir J, Laviolette M, Molet S, Zhu Z, Olivenstein R et al. IL-11 expression is increased in severe asthma: Association with epithelial cells and eosinophils. The Journal of Allergy and Clinical Immunology. 2000;105(2 I):232-238.
Minshall, Eleanor ; Chakir, Jamila ; Laviolette, Michel ; Molet, Sophie ; Zhu, Zhou ; Olivenstein, Ron ; Elias, Jack A. ; Hamid, Qutayba. / IL-11 expression is increased in severe asthma : Association with epithelial cells and eosinophils. In: The Journal of Allergy and Clinical Immunology. 2000 ; Vol. 105, No. 2 I. pp. 232-238.
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abstract = "Background: IL-11 is a pleiotropic cytokine produced by a variety of stromal cells. Targeted overexpression of this cytokine in mice results in a remodeling of the airways and the development of airway hyperresponsiveness and airway obstruction. Objectives: Because these alterations mimic important pathologic and physiologic changes in the airways of some asthmatic patients, we investigated the expression of IL-11 messenger RNA (mRNA) within the airways of patients with mild to severe asthma and nonasthmatic control subjects. Methods: Fiberoptic bronchoscopy to obtain bronchial biopsy specimens was performed on patients with mild (n = 13), moderate (n = 10), and severe (n = 9) asthma and on nonasthmatic control subjects (n = 9). Results: These patients differed in their extent of airway fibrosis with types I and III collagens being noted in greater quantities in the biopsy specimens from the severe and moderate asthmatics than in those from controls (P <.05). IL-11 mRNA expression was observed in the epithelial and subepithelial layers of asthmatic and nonasthmatic control subjects. The number of cells within the epithelium and subepithelium expressing IL-11 mRNA was greater in those with moderate and severe asthma compared with mild asthma and nonasthmatic subjects (P <.001). There were also greater numbers of IL-11 mRNA-positive cells within the subepithelium in severe compared with moderate asthma (P <.001). Immunostaining for IL-11 within the airway tissues confirmed translation of the mRNA into IL-11-immunoreactive protein in airway epithelial cells. Colocalization of IL-11 mRNA and immunoreactivity with resident inflammatory cells demonstrated that this cytokine was also expressed by major basic protein-positiVe eosinophils. Conclusion: These results suggest that IL-11 is involved in the chronic remodeling seen in asthmatic airways and is associated with increasing severity of the disease.",
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T1 - IL-11 expression is increased in severe asthma

T2 - Association with epithelial cells and eosinophils

AU - Minshall, Eleanor

AU - Chakir, Jamila

AU - Laviolette, Michel

AU - Molet, Sophie

AU - Zhu, Zhou

AU - Olivenstein, Ron

AU - Elias, Jack A.

AU - Hamid, Qutayba

PY - 2000

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N2 - Background: IL-11 is a pleiotropic cytokine produced by a variety of stromal cells. Targeted overexpression of this cytokine in mice results in a remodeling of the airways and the development of airway hyperresponsiveness and airway obstruction. Objectives: Because these alterations mimic important pathologic and physiologic changes in the airways of some asthmatic patients, we investigated the expression of IL-11 messenger RNA (mRNA) within the airways of patients with mild to severe asthma and nonasthmatic control subjects. Methods: Fiberoptic bronchoscopy to obtain bronchial biopsy specimens was performed on patients with mild (n = 13), moderate (n = 10), and severe (n = 9) asthma and on nonasthmatic control subjects (n = 9). Results: These patients differed in their extent of airway fibrosis with types I and III collagens being noted in greater quantities in the biopsy specimens from the severe and moderate asthmatics than in those from controls (P <.05). IL-11 mRNA expression was observed in the epithelial and subepithelial layers of asthmatic and nonasthmatic control subjects. The number of cells within the epithelium and subepithelium expressing IL-11 mRNA was greater in those with moderate and severe asthma compared with mild asthma and nonasthmatic subjects (P <.001). There were also greater numbers of IL-11 mRNA-positive cells within the subepithelium in severe compared with moderate asthma (P <.001). Immunostaining for IL-11 within the airway tissues confirmed translation of the mRNA into IL-11-immunoreactive protein in airway epithelial cells. Colocalization of IL-11 mRNA and immunoreactivity with resident inflammatory cells demonstrated that this cytokine was also expressed by major basic protein-positiVe eosinophils. Conclusion: These results suggest that IL-11 is involved in the chronic remodeling seen in asthmatic airways and is associated with increasing severity of the disease.

AB - Background: IL-11 is a pleiotropic cytokine produced by a variety of stromal cells. Targeted overexpression of this cytokine in mice results in a remodeling of the airways and the development of airway hyperresponsiveness and airway obstruction. Objectives: Because these alterations mimic important pathologic and physiologic changes in the airways of some asthmatic patients, we investigated the expression of IL-11 messenger RNA (mRNA) within the airways of patients with mild to severe asthma and nonasthmatic control subjects. Methods: Fiberoptic bronchoscopy to obtain bronchial biopsy specimens was performed on patients with mild (n = 13), moderate (n = 10), and severe (n = 9) asthma and on nonasthmatic control subjects (n = 9). Results: These patients differed in their extent of airway fibrosis with types I and III collagens being noted in greater quantities in the biopsy specimens from the severe and moderate asthmatics than in those from controls (P <.05). IL-11 mRNA expression was observed in the epithelial and subepithelial layers of asthmatic and nonasthmatic control subjects. The number of cells within the epithelium and subepithelium expressing IL-11 mRNA was greater in those with moderate and severe asthma compared with mild asthma and nonasthmatic subjects (P <.001). There were also greater numbers of IL-11 mRNA-positive cells within the subepithelium in severe compared with moderate asthma (P <.001). Immunostaining for IL-11 within the airway tissues confirmed translation of the mRNA into IL-11-immunoreactive protein in airway epithelial cells. Colocalization of IL-11 mRNA and immunoreactivity with resident inflammatory cells demonstrated that this cytokine was also expressed by major basic protein-positiVe eosinophils. Conclusion: These results suggest that IL-11 is involved in the chronic remodeling seen in asthmatic airways and is associated with increasing severity of the disease.

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KW - Severe asthma

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