IL-10 Exerts Suppressive and Enhancing Effects on Antifungal Activity of Mononuclear Phagocytes Against Aspergillus fumigatus

Emmanuel Roilides, Anastasia Dimitriadou, Isaac Kadiltsoglou, Tin Sein, John Karpouzas, Philip A. Pizzo, Thomas J. Walsh

Research output: Contribution to journalArticle

Abstract

Invasive aspergillosis has emerged as a frequent and serious infection in immunocompromised patients. We studied the effects of human IL-10 on antifungal activity of monocytes (MNCs) from healthy adults against Aspergillus fumigatus after incubation with IL-10 at 37°C for 2 to 4 days. IL-10 (2-20 ng/ml)-pretreated MNCs exhibited approximately 40% suppression of superoxide anion (O2 -) production in response to PMA and FMLP (p ≤ 0.003), and anti-IL-10 containing supernatant neutralized the IL-10 effect. IL-10 (20 ng/ml)-pretreated MNCs exhibited decreased damage of Aspergillus hyphae after 2 to 4 days (55-98% decrease, p ≤ 0.04). The MNC phagocytic activity against conidia, as assessed by microscopy (percentage of phagocytosing MNCs and number of intracellular conidia per MNC) as well as by colony counting (colonies grown from intracellular conidia), was enhanced by 127% (p = 0.006), 14% (p = 0.03), and 23% (p = 0.009), respectively. MNC capacity to inhibit intracellular germination was marginally enhanced (p = 0.04) and intracellular conidiocidal activity was unaffected by IL-10. IL-4 (5 ng/ml) did not change the up-regulatory IL-10 effect on phagocytosis. IFN-γ (25 ng/ml) and granulocyte-macrophage CSF (20 ng/ml), but not macrophage CSF (15 ng/ml), appeared to counteract suppressive IL-10 effects. Thus, IL-10 suppresses oxidative burst and antifungal activity of MNCs against Aspergillus hyphae, while increasing their phagocytic activity. These findings further elucidate a potential role of IL-10 in the pathogenesis of invasive aspergillosis, which may lead to new treatment strategies.

Original languageEnglish (US)
Pages (from-to)322-329
Number of pages8
JournalJournal of Immunology
Volume158
Issue number1
StatePublished - Jan 1 1997
Externally publishedYes

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Aspergillus fumigatus
Phagocytes
Interleukin-10
Monocytes
Fungal Spores
Aspergillosis
Hyphae
Aspergillus
Phagocytosis
Macrophages
Respiratory Burst
Interleukin-5
Immunocompromised Host
Germination
Granulocytes
Superoxides
Interleukin-4
Interleukin-2
Microscopy

ASJC Scopus subject areas

  • Immunology

Cite this

Roilides, E., Dimitriadou, A., Kadiltsoglou, I., Sein, T., Karpouzas, J., Pizzo, P. A., & Walsh, T. J. (1997). IL-10 Exerts Suppressive and Enhancing Effects on Antifungal Activity of Mononuclear Phagocytes Against Aspergillus fumigatus. Journal of Immunology, 158(1), 322-329.

IL-10 Exerts Suppressive and Enhancing Effects on Antifungal Activity of Mononuclear Phagocytes Against Aspergillus fumigatus. / Roilides, Emmanuel; Dimitriadou, Anastasia; Kadiltsoglou, Isaac; Sein, Tin; Karpouzas, John; Pizzo, Philip A.; Walsh, Thomas J.

In: Journal of Immunology, Vol. 158, No. 1, 01.01.1997, p. 322-329.

Research output: Contribution to journalArticle

Roilides, E, Dimitriadou, A, Kadiltsoglou, I, Sein, T, Karpouzas, J, Pizzo, PA & Walsh, TJ 1997, 'IL-10 Exerts Suppressive and Enhancing Effects on Antifungal Activity of Mononuclear Phagocytes Against Aspergillus fumigatus', Journal of Immunology, vol. 158, no. 1, pp. 322-329.
Roilides E, Dimitriadou A, Kadiltsoglou I, Sein T, Karpouzas J, Pizzo PA et al. IL-10 Exerts Suppressive and Enhancing Effects on Antifungal Activity of Mononuclear Phagocytes Against Aspergillus fumigatus. Journal of Immunology. 1997 Jan 1;158(1):322-329.
Roilides, Emmanuel ; Dimitriadou, Anastasia ; Kadiltsoglou, Isaac ; Sein, Tin ; Karpouzas, John ; Pizzo, Philip A. ; Walsh, Thomas J. / IL-10 Exerts Suppressive and Enhancing Effects on Antifungal Activity of Mononuclear Phagocytes Against Aspergillus fumigatus. In: Journal of Immunology. 1997 ; Vol. 158, No. 1. pp. 322-329.
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abstract = "Invasive aspergillosis has emerged as a frequent and serious infection in immunocompromised patients. We studied the effects of human IL-10 on antifungal activity of monocytes (MNCs) from healthy adults against Aspergillus fumigatus after incubation with IL-10 at 37°C for 2 to 4 days. IL-10 (2-20 ng/ml)-pretreated MNCs exhibited approximately 40{\%} suppression of superoxide anion (O2 -) production in response to PMA and FMLP (p ≤ 0.003), and anti-IL-10 containing supernatant neutralized the IL-10 effect. IL-10 (20 ng/ml)-pretreated MNCs exhibited decreased damage of Aspergillus hyphae after 2 to 4 days (55-98{\%} decrease, p ≤ 0.04). The MNC phagocytic activity against conidia, as assessed by microscopy (percentage of phagocytosing MNCs and number of intracellular conidia per MNC) as well as by colony counting (colonies grown from intracellular conidia), was enhanced by 127{\%} (p = 0.006), 14{\%} (p = 0.03), and 23{\%} (p = 0.009), respectively. MNC capacity to inhibit intracellular germination was marginally enhanced (p = 0.04) and intracellular conidiocidal activity was unaffected by IL-10. IL-4 (5 ng/ml) did not change the up-regulatory IL-10 effect on phagocytosis. IFN-γ (25 ng/ml) and granulocyte-macrophage CSF (20 ng/ml), but not macrophage CSF (15 ng/ml), appeared to counteract suppressive IL-10 effects. Thus, IL-10 suppresses oxidative burst and antifungal activity of MNCs against Aspergillus hyphae, while increasing their phagocytic activity. These findings further elucidate a potential role of IL-10 in the pathogenesis of invasive aspergillosis, which may lead to new treatment strategies.",
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