IKKβ 2 phosphorylation regulates RPS3 nuclear translocation and NF-κ B function during infection with Escherichia coli strain O157: H7

Fengyi Wan, Amanda Weaver, Xiaofei Gao, Michael Bern, Philip R. Hardwidge, Michael J. Lenardo

Research output: Contribution to journalArticle

Abstract

NF-β °B is a major gene regulator in immune responses, and ribosomal protein S3 (RPS3) is an NF-β °B subunit that directs specific gene transcription. However, it is unknown how nuclear translocation of RPS3 is regulated. Here we report that phosphorylation of RPS3 Ser209 by the kinase IKKβ 2 was crucial for nuclear localization of RPS3 in response to activating stimuli. Moreover, virulence protein NleH1 of the foodborne pathogen Escherichia coli strain O157:H7 specifically inhibited phosphorylation of RPS3 Ser209 and blocked RPS3 function, thereby promoting bacterial colonization and diarrhea but resulting in less mortality in a gnotobiotic piglet-infection model. Thus, the IKKβ 2-dependent modification of a specific amino acid in RPS3 promoted specific NF-κB functions that underlie the molecular pathogenetic mechanisms of E. coli O157:H7.

Original languageEnglish (US)
Pages (from-to)335-344
Number of pages10
JournalNature Immunology
Volume12
Issue number4
DOIs
StatePublished - Apr 2011

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Escherichia coli O157
Phosphorylation
Infection
I-kappa B Kinase
Germ-Free Life
Regulator Genes
ribosomal protein S3
Virulence
Diarrhea
Amino Acids
Mortality
Genes

ASJC Scopus subject areas

  • Immunology

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IKKβ 2 phosphorylation regulates RPS3 nuclear translocation and NF-κ B function during infection with Escherichia coli strain O157 : H7. / Wan, Fengyi; Weaver, Amanda; Gao, Xiaofei; Bern, Michael; Hardwidge, Philip R.; Lenardo, Michael J.

In: Nature Immunology, Vol. 12, No. 4, 04.2011, p. 335-344.

Research output: Contribution to journalArticle

Wan, Fengyi ; Weaver, Amanda ; Gao, Xiaofei ; Bern, Michael ; Hardwidge, Philip R. ; Lenardo, Michael J. / IKKβ 2 phosphorylation regulates RPS3 nuclear translocation and NF-κ B function during infection with Escherichia coli strain O157 : H7. In: Nature Immunology. 2011 ; Vol. 12, No. 4. pp. 335-344.
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