IgG binding and expression of its receptor in rat intestine during postnatal development

Ravinder Kaur Gill, Safrun Mahmood, Chhinder Sodhi, J. P. Nagpaul, Akhtar Mahmood

Research output: Contribution to journalArticle

Abstract

The binding of 125I labelled IgG to the microvillus membranes (MVM) has been studied during postnatal development of rat intestine. The levels of mRNA encoding IgG receptor were also analyzed by liquid hybridization under these conditions. The IgG binding to MVM reached maximum levels by day 12 and showed a gradual decline upon weaning. The FcRn mRNA was markedly low in adult rats and was maximum during second week of postnatal development. Administration of cortisone or thyroxine to suckling rats, induced precocious decline of both IgG binding and the receptor expression. However, insulin administration did not affect the receptor expression. Scatchard analysis of IgG binding to MVM in cortisone injected pups revealed that the observed inhibition in IgG binding was a consequence of a decrease, both in the affinity constant (-Ka) as well as in the number of receptor sites (n) while thyroxine administration caused a reduction in the number of receptor sites from 2.29 in control to 1.14 nmoles/mg protein in thyroxine injected pups. These observations indicate that expression of IgG receptor during postnatal development is a hormone regulated process.

Original languageEnglish (US)
Pages (from-to)252-257
Number of pages6
JournalIndian journal of biochemistry & biophysics
Volume36
Issue number4
StatePublished - Aug 1999
Externally publishedYes

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IgG Receptors
Intestines
Rats
Microvilli
Immunoglobulin G
Thyroxine
Cortisone
Membranes
Messenger RNA
Weaning
Hormones
Insulin
Liquids
Proteins

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics

Cite this

IgG binding and expression of its receptor in rat intestine during postnatal development. / Gill, Ravinder Kaur; Mahmood, Safrun; Sodhi, Chhinder; Nagpaul, J. P.; Mahmood, Akhtar.

In: Indian journal of biochemistry & biophysics, Vol. 36, No. 4, 08.1999, p. 252-257.

Research output: Contribution to journalArticle

Gill, Ravinder Kaur ; Mahmood, Safrun ; Sodhi, Chhinder ; Nagpaul, J. P. ; Mahmood, Akhtar. / IgG binding and expression of its receptor in rat intestine during postnatal development. In: Indian journal of biochemistry & biophysics. 1999 ; Vol. 36, No. 4. pp. 252-257.
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