IgG anti-IgE from atopic dermatitis induces mediator release from basophils and mast cells

Gianni Marone, Vincenzo Casolaro, Roberto Paganelli, Isabella Quinti

Research output: Contribution to journalArticle

Abstract

IgG antibodies containing anti-IgE activity isolated from a patient with atopic dermatitis (H-aIgE) induced mediator release from human basophils and mast cells isolated from skin and lung tissues. The release of histamine was calcium and temperature-dependent and did not involve cytotoxicity. There was an excellent correlation (r = 0.88; p <0.001) between the maximum percent histamine release from human basophils induced by rabbit anti-IgE (R-aIgE) and H-aIgE. H-aIgE was approximately 30 times more potent than R-aIgE in inducing mediator release from human basophils, skin, and lung mast cells. H-aIgE specifically desensitized human basophils to a subsequent challenge with both H-aIgE and R-aIgE. Lactic acid removal of IgE from human basophils blocked the releasing activity of both R-aIgE and H-aIgE. Passive sensitization with hyperimmune sera or purified IgE myeloma restored the response of basophils to both R-aIgE and H-aIgE. IgE purified from three different myeloma patients concentration-dependently blocked the histamine releasing activity of both R-aIgE and H-aIgE.

Original languageEnglish (US)
Pages (from-to)246-252
Number of pages7
JournalJournal of Investigative Dermatology
Volume93
Issue number2
StatePublished - Aug 1989
Externally publishedYes

ASJC Scopus subject areas

  • Dermatology

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