IgG anti-IgE from atopic dermatitis induces mediator release from basophils and mast cells

Gianni Marone, Vincenzo Casolaro, Roberto Paganelli, Isabella Quinti

Research output: Contribution to journalArticle

Abstract

IgG antibodies containing anti-IgE activity isolated from a patient with atopic dermatitis (H-aIgE) induced mediator release from human basophils and mast cells isolated from skin and lung tissues. The release of histamine was calcium and temperature-dependent and did not involve cytotoxicity. There was an excellent correlation (r = 0.88; p <0.001) between the maximum percent histamine release from human basophils induced by rabbit anti-IgE (R-aIgE) and H-aIgE. H-aIgE was approximately 30 times more potent than R-aIgE in inducing mediator release from human basophils, skin, and lung mast cells. H-aIgE specifically desensitized human basophils to a subsequent challenge with both H-aIgE and R-aIgE. Lactic acid removal of IgE from human basophils blocked the releasing activity of both R-aIgE and H-aIgE. Passive sensitization with hyperimmune sera or purified IgE myeloma restored the response of basophils to both R-aIgE and H-aIgE. IgE purified from three different myeloma patients concentration-dependently blocked the histamine releasing activity of both R-aIgE and H-aIgE.

Original languageEnglish (US)
Pages (from-to)246-252
Number of pages7
JournalJournal of Investigative Dermatology
Volume93
Issue number2
StatePublished - Aug 1989
Externally publishedYes

Fingerprint

Basophils
Atopic Dermatitis
Mast Cells
Immunoglobulin G
Rabbits
Immunoglobulin E
Histamine
Histamine Release
Skin
Lung
Cytotoxicity
anti-IgE antibodies
Lactic Acid
Tissue
Calcium
Temperature
Antibodies
Serum

ASJC Scopus subject areas

  • Dermatology

Cite this

IgG anti-IgE from atopic dermatitis induces mediator release from basophils and mast cells. / Marone, Gianni; Casolaro, Vincenzo; Paganelli, Roberto; Quinti, Isabella.

In: Journal of Investigative Dermatology, Vol. 93, No. 2, 08.1989, p. 246-252.

Research output: Contribution to journalArticle

Marone, G, Casolaro, V, Paganelli, R & Quinti, I 1989, 'IgG anti-IgE from atopic dermatitis induces mediator release from basophils and mast cells', Journal of Investigative Dermatology, vol. 93, no. 2, pp. 246-252.
Marone, Gianni ; Casolaro, Vincenzo ; Paganelli, Roberto ; Quinti, Isabella. / IgG anti-IgE from atopic dermatitis induces mediator release from basophils and mast cells. In: Journal of Investigative Dermatology. 1989 ; Vol. 93, No. 2. pp. 246-252.
@article{cd80fc862c704c7bb6919454eb420148,
title = "IgG anti-IgE from atopic dermatitis induces mediator release from basophils and mast cells",
abstract = "IgG antibodies containing anti-IgE activity isolated from a patient with atopic dermatitis (H-aIgE) induced mediator release from human basophils and mast cells isolated from skin and lung tissues. The release of histamine was calcium and temperature-dependent and did not involve cytotoxicity. There was an excellent correlation (r = 0.88; p <0.001) between the maximum percent histamine release from human basophils induced by rabbit anti-IgE (R-aIgE) and H-aIgE. H-aIgE was approximately 30 times more potent than R-aIgE in inducing mediator release from human basophils, skin, and lung mast cells. H-aIgE specifically desensitized human basophils to a subsequent challenge with both H-aIgE and R-aIgE. Lactic acid removal of IgE from human basophils blocked the releasing activity of both R-aIgE and H-aIgE. Passive sensitization with hyperimmune sera or purified IgE myeloma restored the response of basophils to both R-aIgE and H-aIgE. IgE purified from three different myeloma patients concentration-dependently blocked the histamine releasing activity of both R-aIgE and H-aIgE.",
author = "Gianni Marone and Vincenzo Casolaro and Roberto Paganelli and Isabella Quinti",
year = "1989",
month = "8",
language = "English (US)",
volume = "93",
pages = "246--252",
journal = "Journal of Investigative Dermatology",
issn = "0022-202X",
publisher = "Nature Publishing Group",
number = "2",

}

TY - JOUR

T1 - IgG anti-IgE from atopic dermatitis induces mediator release from basophils and mast cells

AU - Marone, Gianni

AU - Casolaro, Vincenzo

AU - Paganelli, Roberto

AU - Quinti, Isabella

PY - 1989/8

Y1 - 1989/8

N2 - IgG antibodies containing anti-IgE activity isolated from a patient with atopic dermatitis (H-aIgE) induced mediator release from human basophils and mast cells isolated from skin and lung tissues. The release of histamine was calcium and temperature-dependent and did not involve cytotoxicity. There was an excellent correlation (r = 0.88; p <0.001) between the maximum percent histamine release from human basophils induced by rabbit anti-IgE (R-aIgE) and H-aIgE. H-aIgE was approximately 30 times more potent than R-aIgE in inducing mediator release from human basophils, skin, and lung mast cells. H-aIgE specifically desensitized human basophils to a subsequent challenge with both H-aIgE and R-aIgE. Lactic acid removal of IgE from human basophils blocked the releasing activity of both R-aIgE and H-aIgE. Passive sensitization with hyperimmune sera or purified IgE myeloma restored the response of basophils to both R-aIgE and H-aIgE. IgE purified from three different myeloma patients concentration-dependently blocked the histamine releasing activity of both R-aIgE and H-aIgE.

AB - IgG antibodies containing anti-IgE activity isolated from a patient with atopic dermatitis (H-aIgE) induced mediator release from human basophils and mast cells isolated from skin and lung tissues. The release of histamine was calcium and temperature-dependent and did not involve cytotoxicity. There was an excellent correlation (r = 0.88; p <0.001) between the maximum percent histamine release from human basophils induced by rabbit anti-IgE (R-aIgE) and H-aIgE. H-aIgE was approximately 30 times more potent than R-aIgE in inducing mediator release from human basophils, skin, and lung mast cells. H-aIgE specifically desensitized human basophils to a subsequent challenge with both H-aIgE and R-aIgE. Lactic acid removal of IgE from human basophils blocked the releasing activity of both R-aIgE and H-aIgE. Passive sensitization with hyperimmune sera or purified IgE myeloma restored the response of basophils to both R-aIgE and H-aIgE. IgE purified from three different myeloma patients concentration-dependently blocked the histamine releasing activity of both R-aIgE and H-aIgE.

UR - http://www.scopus.com/inward/record.url?scp=0024400855&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024400855&partnerID=8YFLogxK

M3 - Article

C2 - 2787823

AN - SCOPUS:0024400855

VL - 93

SP - 246

EP - 252

JO - Journal of Investigative Dermatology

JF - Journal of Investigative Dermatology

SN - 0022-202X

IS - 2

ER -