Abstract
IgG antibodies containing anti-IgE activity isolated from a patient with atopic dermatitis (H-aIgE) induced mediator release from human basophils and mast cells isolated from skin and lung tissues. The release of histamine was calcium and temperature-dependent and did not involve cytotoxicity. There was an excellent correlation (r = 0.88; p <0.001) between the maximum percent histamine release from human basophils induced by rabbit anti-IgE (R-aIgE) and H-aIgE. H-aIgE was approximately 30 times more potent than R-aIgE in inducing mediator release from human basophils, skin, and lung mast cells. H-aIgE specifically desensitized human basophils to a subsequent challenge with both H-aIgE and R-aIgE. Lactic acid removal of IgE from human basophils blocked the releasing activity of both R-aIgE and H-aIgE. Passive sensitization with hyperimmune sera or purified IgE myeloma restored the response of basophils to both R-aIgE and H-aIgE. IgE purified from three different myeloma patients concentration-dependently blocked the histamine releasing activity of both R-aIgE and H-aIgE.
Original language | English (US) |
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Pages (from-to) | 246-252 |
Number of pages | 7 |
Journal | Journal of Investigative Dermatology |
Volume | 93 |
Issue number | 2 |
State | Published - Aug 1989 |
Externally published | Yes |
ASJC Scopus subject areas
- Dermatology