IGF-I inhibits burst mass of pulsatile insulin secretion at supraphysiological and low IGF-I infusion rates

Niels Pørksen, Mehboob Hussain, T. L. Bianda, Birgit Nyholm, Jens S. Christiansen, Peter C. Butler, Johannes D. Veldhuis, E. Rudi Froesch, Ole Schmitz

Research output: Contribution to journalArticle

Abstract

Insulin-like growth factor I (IGF-I) shares structural and functional features with insulin, affects carbohydrate metabolism, and inhibits insulin secretion. Insulin secretion is pulsatile, and it is regulated by changing frequency and/or mass of secretory bursts. To examine the mechanism of IGF- I's inhibition of insulin secretion, eight healthy volunteers were studied three times. During glucose infusion (2.5 mg · kg-1 · min-1) blood was sampled minutely at time 75-200 rain for triplicate insulin concentration measurements by enzyme-linked immunosorbent assay (ELISA; coefficient of variation 2.1%). Time 125 min infusion of saline, low-dose IGF-I (0.025 μg · kg-1 · min-1) or high-dose IGF-I (0.15 μg · kg-1 · min-1) was commenced and continued until 200 min. Data were compared before (75-125 min) vs. during infusion (150-200 min). Insulin concentration time series were deconvolved, using validated pulse-detection criteria, to assess insulin secretory burst mass and frequency. During saline infusion no time effect occurred. After IGF-I infusion, serum C-peptide decreased (582 ± 85 vs. 481 ± 82 pM, low-dose IGF-I, P <0.05; 539 ± 84 vs. 427 ± 69 pM, high-dose IGF-I, P <0.01). Total insulin secretion rates decreased by 17 and 21%, respectively, via specific inhibition of the insulin secretory burst mass (31 ± 8 vs. 20 ± 4 pmol/ml, low-dose IGF-I, P = 0.06; 22 ± 4 vs. 17 ± 3 pmol/ml, high-dose IGF-I, P <0.05), whereas the frequency was not affected (10.5 ± 1.3 vs. 10.7 ± 1.3 pulses/h, low-dose IGF-I, P = 0.85; 8.7 ± 1.0 vs. 11.1 ± 1.2 min/pulse, high-dose IGF-I, P = 0.15). We conclude that IGF- I inhibits pulsatile insulin secretion by specific inhibition of mass but not frequency of secretory bursts.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Endocrinology and Metabolism
Volume272
Issue number3 35-3
StatePublished - Mar 1997
Externally publishedYes

Fingerprint

Insulin-Like Growth Factor I
Insulin
Enzyme-Linked Immunosorbent Assay
Immunosorbents
Rain
C-Peptide
Carbohydrate Metabolism
Time series
Assays
Healthy Volunteers
Blood
Glucose

Keywords

  • amplitude
  • C-peptide
  • dose response
  • pulsatility
  • somatostatin

ASJC Scopus subject areas

  • Physiology
  • Endocrinology
  • Biochemistry
  • Physiology (medical)

Cite this

IGF-I inhibits burst mass of pulsatile insulin secretion at supraphysiological and low IGF-I infusion rates. / Pørksen, Niels; Hussain, Mehboob; Bianda, T. L.; Nyholm, Birgit; Christiansen, Jens S.; Butler, Peter C.; Veldhuis, Johannes D.; Froesch, E. Rudi; Schmitz, Ole.

In: American Journal of Physiology - Endocrinology and Metabolism, Vol. 272, No. 3 35-3, 03.1997.

Research output: Contribution to journalArticle

Pørksen, N, Hussain, M, Bianda, TL, Nyholm, B, Christiansen, JS, Butler, PC, Veldhuis, JD, Froesch, ER & Schmitz, O 1997, 'IGF-I inhibits burst mass of pulsatile insulin secretion at supraphysiological and low IGF-I infusion rates', American Journal of Physiology - Endocrinology and Metabolism, vol. 272, no. 3 35-3.
Pørksen, Niels ; Hussain, Mehboob ; Bianda, T. L. ; Nyholm, Birgit ; Christiansen, Jens S. ; Butler, Peter C. ; Veldhuis, Johannes D. ; Froesch, E. Rudi ; Schmitz, Ole. / IGF-I inhibits burst mass of pulsatile insulin secretion at supraphysiological and low IGF-I infusion rates. In: American Journal of Physiology - Endocrinology and Metabolism. 1997 ; Vol. 272, No. 3 35-3.
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abstract = "Insulin-like growth factor I (IGF-I) shares structural and functional features with insulin, affects carbohydrate metabolism, and inhibits insulin secretion. Insulin secretion is pulsatile, and it is regulated by changing frequency and/or mass of secretory bursts. To examine the mechanism of IGF- I's inhibition of insulin secretion, eight healthy volunteers were studied three times. During glucose infusion (2.5 mg · kg-1 · min-1) blood was sampled minutely at time 75-200 rain for triplicate insulin concentration measurements by enzyme-linked immunosorbent assay (ELISA; coefficient of variation 2.1{\%}). Time 125 min infusion of saline, low-dose IGF-I (0.025 μg · kg-1 · min-1) or high-dose IGF-I (0.15 μg · kg-1 · min-1) was commenced and continued until 200 min. Data were compared before (75-125 min) vs. during infusion (150-200 min). Insulin concentration time series were deconvolved, using validated pulse-detection criteria, to assess insulin secretory burst mass and frequency. During saline infusion no time effect occurred. After IGF-I infusion, serum C-peptide decreased (582 ± 85 vs. 481 ± 82 pM, low-dose IGF-I, P <0.05; 539 ± 84 vs. 427 ± 69 pM, high-dose IGF-I, P <0.01). Total insulin secretion rates decreased by 17 and 21{\%}, respectively, via specific inhibition of the insulin secretory burst mass (31 ± 8 vs. 20 ± 4 pmol/ml, low-dose IGF-I, P = 0.06; 22 ± 4 vs. 17 ± 3 pmol/ml, high-dose IGF-I, P <0.05), whereas the frequency was not affected (10.5 ± 1.3 vs. 10.7 ± 1.3 pulses/h, low-dose IGF-I, P = 0.85; 8.7 ± 1.0 vs. 11.1 ± 1.2 min/pulse, high-dose IGF-I, P = 0.15). We conclude that IGF- I inhibits pulsatile insulin secretion by specific inhibition of mass but not frequency of secretory bursts.",
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AU - Pørksen, Niels

AU - Hussain, Mehboob

AU - Bianda, T. L.

AU - Nyholm, Birgit

AU - Christiansen, Jens S.

AU - Butler, Peter C.

AU - Veldhuis, Johannes D.

AU - Froesch, E. Rudi

AU - Schmitz, Ole

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N2 - Insulin-like growth factor I (IGF-I) shares structural and functional features with insulin, affects carbohydrate metabolism, and inhibits insulin secretion. Insulin secretion is pulsatile, and it is regulated by changing frequency and/or mass of secretory bursts. To examine the mechanism of IGF- I's inhibition of insulin secretion, eight healthy volunteers were studied three times. During glucose infusion (2.5 mg · kg-1 · min-1) blood was sampled minutely at time 75-200 rain for triplicate insulin concentration measurements by enzyme-linked immunosorbent assay (ELISA; coefficient of variation 2.1%). Time 125 min infusion of saline, low-dose IGF-I (0.025 μg · kg-1 · min-1) or high-dose IGF-I (0.15 μg · kg-1 · min-1) was commenced and continued until 200 min. Data were compared before (75-125 min) vs. during infusion (150-200 min). Insulin concentration time series were deconvolved, using validated pulse-detection criteria, to assess insulin secretory burst mass and frequency. During saline infusion no time effect occurred. After IGF-I infusion, serum C-peptide decreased (582 ± 85 vs. 481 ± 82 pM, low-dose IGF-I, P <0.05; 539 ± 84 vs. 427 ± 69 pM, high-dose IGF-I, P <0.01). Total insulin secretion rates decreased by 17 and 21%, respectively, via specific inhibition of the insulin secretory burst mass (31 ± 8 vs. 20 ± 4 pmol/ml, low-dose IGF-I, P = 0.06; 22 ± 4 vs. 17 ± 3 pmol/ml, high-dose IGF-I, P <0.05), whereas the frequency was not affected (10.5 ± 1.3 vs. 10.7 ± 1.3 pulses/h, low-dose IGF-I, P = 0.85; 8.7 ± 1.0 vs. 11.1 ± 1.2 min/pulse, high-dose IGF-I, P = 0.15). We conclude that IGF- I inhibits pulsatile insulin secretion by specific inhibition of mass but not frequency of secretory bursts.

AB - Insulin-like growth factor I (IGF-I) shares structural and functional features with insulin, affects carbohydrate metabolism, and inhibits insulin secretion. Insulin secretion is pulsatile, and it is regulated by changing frequency and/or mass of secretory bursts. To examine the mechanism of IGF- I's inhibition of insulin secretion, eight healthy volunteers were studied three times. During glucose infusion (2.5 mg · kg-1 · min-1) blood was sampled minutely at time 75-200 rain for triplicate insulin concentration measurements by enzyme-linked immunosorbent assay (ELISA; coefficient of variation 2.1%). Time 125 min infusion of saline, low-dose IGF-I (0.025 μg · kg-1 · min-1) or high-dose IGF-I (0.15 μg · kg-1 · min-1) was commenced and continued until 200 min. Data were compared before (75-125 min) vs. during infusion (150-200 min). Insulin concentration time series were deconvolved, using validated pulse-detection criteria, to assess insulin secretory burst mass and frequency. During saline infusion no time effect occurred. After IGF-I infusion, serum C-peptide decreased (582 ± 85 vs. 481 ± 82 pM, low-dose IGF-I, P <0.05; 539 ± 84 vs. 427 ± 69 pM, high-dose IGF-I, P <0.01). Total insulin secretion rates decreased by 17 and 21%, respectively, via specific inhibition of the insulin secretory burst mass (31 ± 8 vs. 20 ± 4 pmol/ml, low-dose IGF-I, P = 0.06; 22 ± 4 vs. 17 ± 3 pmol/ml, high-dose IGF-I, P <0.05), whereas the frequency was not affected (10.5 ± 1.3 vs. 10.7 ± 1.3 pulses/h, low-dose IGF-I, P = 0.85; 8.7 ± 1.0 vs. 11.1 ± 1.2 min/pulse, high-dose IGF-I, P = 0.15). We conclude that IGF- I inhibits pulsatile insulin secretion by specific inhibition of mass but not frequency of secretory bursts.

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