IgE formation in the rat following infection with Nippostrongylus brasiliensis. I. Proliferation and differentiation of IgE-bearing cells

Teruko Ishizaka, Joseph F. Urban, Kimishige Ishizaka

Research output: Contribution to journalArticle

Abstract

Sprague-Dawley rats were infected with Nippostrongylus brasiliensis larvae, and IgE formation was studied. Before infection, the serum IgE level was less than 0.4 μg/ml. The IgE level began to increase from the 10th day of infection, reached its maximum (50-100 μg/ml) at the 14th day and gradually declined. Reinfection of the rats resulted in an increase of the serum IgE level within 7 days. The IgE antibody response to N. brasiliensis antigens did not parallel the increase of IgE synthesis. In most animals, the antibody became detectable in the serum at the 21st day when the total IgE level already began to decrease. The animals showed a secondary IgE antibody response upon reinfection. Both mesenteric lymph nodes and spleen cell suspensions were examined for the presence of IgE-bearing cells (IgE-B cells) and IgE-forming cells by fluorescent antibody technique. The IgE-bearing lymphocytes became detectable in the mesenteric lymph nodes and spleen at the 8th day of infection. The proportion of the IgE-B cells in nonadherent cell population gradually increased and reached maximum at the 14th day; about 20% of immunoglobulin (Ig)-bearing cells in the mesenteric lymph nodes and 10% of Ig-bearing cells in spleen bore IgE on their surface. Evidence was obtained that these lymphocytes synthesized IgE. The IgE-forming cells were detected in both mesenteric lymph nodes and spleen of the infected animals. The number of IgE-forming cells was greater in the mesenteric lymph nodes than in spleen, indicating that the regional lymph nodes are the major source of serum IgE in the N. brasiliensis-infected animals.

Original languageEnglish (US)
Pages (from-to)248-261
Number of pages14
JournalCellular Immunology
Volume22
Issue number2
DOIs
StatePublished - Mar 15 1976

Fingerprint

Nippostrongylus
Immunoglobulin E
Infection
Lymph Nodes
Spleen
Serum
Antibody Formation
Immunoglobulins
Lymphocytes

ASJC Scopus subject areas

  • Cell Biology
  • Immunology

Cite this

IgE formation in the rat following infection with Nippostrongylus brasiliensis. I. Proliferation and differentiation of IgE-bearing cells. / Ishizaka, Teruko; Urban, Joseph F.; Ishizaka, Kimishige.

In: Cellular Immunology, Vol. 22, No. 2, 15.03.1976, p. 248-261.

Research output: Contribution to journalArticle

Ishizaka, Teruko ; Urban, Joseph F. ; Ishizaka, Kimishige. / IgE formation in the rat following infection with Nippostrongylus brasiliensis. I. Proliferation and differentiation of IgE-bearing cells. In: Cellular Immunology. 1976 ; Vol. 22, No. 2. pp. 248-261.
@article{496407a4d2df4844b72e2be5093c3367,
title = "IgE formation in the rat following infection with Nippostrongylus brasiliensis. I. Proliferation and differentiation of IgE-bearing cells",
abstract = "Sprague-Dawley rats were infected with Nippostrongylus brasiliensis larvae, and IgE formation was studied. Before infection, the serum IgE level was less than 0.4 μg/ml. The IgE level began to increase from the 10th day of infection, reached its maximum (50-100 μg/ml) at the 14th day and gradually declined. Reinfection of the rats resulted in an increase of the serum IgE level within 7 days. The IgE antibody response to N. brasiliensis antigens did not parallel the increase of IgE synthesis. In most animals, the antibody became detectable in the serum at the 21st day when the total IgE level already began to decrease. The animals showed a secondary IgE antibody response upon reinfection. Both mesenteric lymph nodes and spleen cell suspensions were examined for the presence of IgE-bearing cells (IgE-B cells) and IgE-forming cells by fluorescent antibody technique. The IgE-bearing lymphocytes became detectable in the mesenteric lymph nodes and spleen at the 8th day of infection. The proportion of the IgE-B cells in nonadherent cell population gradually increased and reached maximum at the 14th day; about 20{\%} of immunoglobulin (Ig)-bearing cells in the mesenteric lymph nodes and 10{\%} of Ig-bearing cells in spleen bore IgE on their surface. Evidence was obtained that these lymphocytes synthesized IgE. The IgE-forming cells were detected in both mesenteric lymph nodes and spleen of the infected animals. The number of IgE-forming cells was greater in the mesenteric lymph nodes than in spleen, indicating that the regional lymph nodes are the major source of serum IgE in the N. brasiliensis-infected animals.",
author = "Teruko Ishizaka and Urban, {Joseph F.} and Kimishige Ishizaka",
year = "1976",
month = "3",
day = "15",
doi = "10.1016/0008-8749(76)90027-7",
language = "English (US)",
volume = "22",
pages = "248--261",
journal = "Cellular Immunology",
issn = "0008-8749",
publisher = "Academic Press Inc.",
number = "2",

}

TY - JOUR

T1 - IgE formation in the rat following infection with Nippostrongylus brasiliensis. I. Proliferation and differentiation of IgE-bearing cells

AU - Ishizaka, Teruko

AU - Urban, Joseph F.

AU - Ishizaka, Kimishige

PY - 1976/3/15

Y1 - 1976/3/15

N2 - Sprague-Dawley rats were infected with Nippostrongylus brasiliensis larvae, and IgE formation was studied. Before infection, the serum IgE level was less than 0.4 μg/ml. The IgE level began to increase from the 10th day of infection, reached its maximum (50-100 μg/ml) at the 14th day and gradually declined. Reinfection of the rats resulted in an increase of the serum IgE level within 7 days. The IgE antibody response to N. brasiliensis antigens did not parallel the increase of IgE synthesis. In most animals, the antibody became detectable in the serum at the 21st day when the total IgE level already began to decrease. The animals showed a secondary IgE antibody response upon reinfection. Both mesenteric lymph nodes and spleen cell suspensions were examined for the presence of IgE-bearing cells (IgE-B cells) and IgE-forming cells by fluorescent antibody technique. The IgE-bearing lymphocytes became detectable in the mesenteric lymph nodes and spleen at the 8th day of infection. The proportion of the IgE-B cells in nonadherent cell population gradually increased and reached maximum at the 14th day; about 20% of immunoglobulin (Ig)-bearing cells in the mesenteric lymph nodes and 10% of Ig-bearing cells in spleen bore IgE on their surface. Evidence was obtained that these lymphocytes synthesized IgE. The IgE-forming cells were detected in both mesenteric lymph nodes and spleen of the infected animals. The number of IgE-forming cells was greater in the mesenteric lymph nodes than in spleen, indicating that the regional lymph nodes are the major source of serum IgE in the N. brasiliensis-infected animals.

AB - Sprague-Dawley rats were infected with Nippostrongylus brasiliensis larvae, and IgE formation was studied. Before infection, the serum IgE level was less than 0.4 μg/ml. The IgE level began to increase from the 10th day of infection, reached its maximum (50-100 μg/ml) at the 14th day and gradually declined. Reinfection of the rats resulted in an increase of the serum IgE level within 7 days. The IgE antibody response to N. brasiliensis antigens did not parallel the increase of IgE synthesis. In most animals, the antibody became detectable in the serum at the 21st day when the total IgE level already began to decrease. The animals showed a secondary IgE antibody response upon reinfection. Both mesenteric lymph nodes and spleen cell suspensions were examined for the presence of IgE-bearing cells (IgE-B cells) and IgE-forming cells by fluorescent antibody technique. The IgE-bearing lymphocytes became detectable in the mesenteric lymph nodes and spleen at the 8th day of infection. The proportion of the IgE-B cells in nonadherent cell population gradually increased and reached maximum at the 14th day; about 20% of immunoglobulin (Ig)-bearing cells in the mesenteric lymph nodes and 10% of Ig-bearing cells in spleen bore IgE on their surface. Evidence was obtained that these lymphocytes synthesized IgE. The IgE-forming cells were detected in both mesenteric lymph nodes and spleen of the infected animals. The number of IgE-forming cells was greater in the mesenteric lymph nodes than in spleen, indicating that the regional lymph nodes are the major source of serum IgE in the N. brasiliensis-infected animals.

UR - http://www.scopus.com/inward/record.url?scp=0017280398&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0017280398&partnerID=8YFLogxK

U2 - 10.1016/0008-8749(76)90027-7

DO - 10.1016/0008-8749(76)90027-7

M3 - Article

C2 - 776414

AN - SCOPUS:0017280398

VL - 22

SP - 248

EP - 261

JO - Cellular Immunology

JF - Cellular Immunology

SN - 0008-8749

IS - 2

ER -