IgD-activated mast cells induce IgE synthesis in B cells in nasal polyps

Guan Ting Zhai, Hai Wang, Jing Xian Li, Ping Ping Cao, Wen Xiu Jiang, Jia Song, Yin Yao, Zhi Chao Wang, Zhe Zheng Wang, Meng Chen Wang, Bo Liao, Qi Miao Feng, Xiang Lu, Heng Wang, Peisong Gao, Zheng Liu

Research output: Contribution to journalArticle

Abstract

Background: Although upregulated expression of local IgD has been reported in patients with chronic rhinosinusitis (CRS), its function is unclear. Objective: We sought to explore the expression and function of soluble IgD in patients with CRS, particularly CRS with nasal polyps. Methods: IgD levels in sinonasal mucosa were analyzed by using RT-PCR and ELISA. Numbers and phenotypes of IgD+ cells were studied by means of immunohistochemistry, immunofluorescence, and flow cytometry. HMC-1 cells, a human mast cell line, and mast cells purified from eosinophilic polyps were cultured alone or with naive B cells purified from peripheral blood. The antigen specificity of nasal IgD was investigated by using ELISA. Results: The mRNA expression of immunoglobulin heavy constant delta gene, numbers of IgD+ cells, and protein levels of secretory IgD in sinonasal mucosa were increased in patients with CRS with or without nasal polyps compared with control subjects. Numbers of IgD+ plasmablasts were increased in both eosinophilic and noneosinophilic polyps, whereas numbers of IgD+ mast cells were only increased in eosinophilic polyps. Cross-linking IgD induced serum preincubated HMC-1 cells and polyp mast cells to produce B-cell activating factor, IL-21, IL-4, and IL-13 and to promote IgM, IgG, IgA, and IgE production from B cells. In eosinophilic polyps expression of those B cell–stimulating factors in mast cells and close contact between mast cells and B cells were found. Moreover, positive correlations of total IgD levels with total IgE levels and eosinophilia and upregulation of specific IgD against house dust mites were discovered in eosinophilic polyps. Conclusion: IgD-activated mast cells can facilitate IgE production and eosinophilic inflammation in patients with CRS with nasal polyps.

Original languageEnglish (US)
Pages (from-to)1489-1499.e23
JournalJournal of Allergy and Clinical Immunology
Volume142
Issue number5
DOIs
StatePublished - Nov 1 2018

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Nasal Polyps
Immunoglobulin D
Mast Cells
Immunoglobulin E
B-Lymphocytes
Polyps
Mucous Membrane
Enzyme-Linked Immunosorbent Assay
B-Cell Activating Factor
Pyroglyphidae
Interleukin-13
Eosinophilia
Nose
Interleukin-4
Immunoglobulin A
Fluorescent Antibody Technique
Immunoglobulin M
Immunoglobulins

Keywords

  • B cell
  • chronic rhinosinusitis
  • eosinophil
  • IgD
  • IgE
  • mast cell
  • nasal polyps

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Zhai, G. T., Wang, H., Li, J. X., Cao, P. P., Jiang, W. X., Song, J., ... Liu, Z. (2018). IgD-activated mast cells induce IgE synthesis in B cells in nasal polyps. Journal of Allergy and Clinical Immunology, 142(5), 1489-1499.e23. https://doi.org/10.1016/j.jaci.2018.07.025

IgD-activated mast cells induce IgE synthesis in B cells in nasal polyps. / Zhai, Guan Ting; Wang, Hai; Li, Jing Xian; Cao, Ping Ping; Jiang, Wen Xiu; Song, Jia; Yao, Yin; Wang, Zhi Chao; Wang, Zhe Zheng; Wang, Meng Chen; Liao, Bo; Feng, Qi Miao; Lu, Xiang; Wang, Heng; Gao, Peisong; Liu, Zheng.

In: Journal of Allergy and Clinical Immunology, Vol. 142, No. 5, 01.11.2018, p. 1489-1499.e23.

Research output: Contribution to journalArticle

Zhai, GT, Wang, H, Li, JX, Cao, PP, Jiang, WX, Song, J, Yao, Y, Wang, ZC, Wang, ZZ, Wang, MC, Liao, B, Feng, QM, Lu, X, Wang, H, Gao, P & Liu, Z 2018, 'IgD-activated mast cells induce IgE synthesis in B cells in nasal polyps', Journal of Allergy and Clinical Immunology, vol. 142, no. 5, pp. 1489-1499.e23. https://doi.org/10.1016/j.jaci.2018.07.025
Zhai, Guan Ting ; Wang, Hai ; Li, Jing Xian ; Cao, Ping Ping ; Jiang, Wen Xiu ; Song, Jia ; Yao, Yin ; Wang, Zhi Chao ; Wang, Zhe Zheng ; Wang, Meng Chen ; Liao, Bo ; Feng, Qi Miao ; Lu, Xiang ; Wang, Heng ; Gao, Peisong ; Liu, Zheng. / IgD-activated mast cells induce IgE synthesis in B cells in nasal polyps. In: Journal of Allergy and Clinical Immunology. 2018 ; Vol. 142, No. 5. pp. 1489-1499.e23.
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abstract = "Background: Although upregulated expression of local IgD has been reported in patients with chronic rhinosinusitis (CRS), its function is unclear. Objective: We sought to explore the expression and function of soluble IgD in patients with CRS, particularly CRS with nasal polyps. Methods: IgD levels in sinonasal mucosa were analyzed by using RT-PCR and ELISA. Numbers and phenotypes of IgD+ cells were studied by means of immunohistochemistry, immunofluorescence, and flow cytometry. HMC-1 cells, a human mast cell line, and mast cells purified from eosinophilic polyps were cultured alone or with naive B cells purified from peripheral blood. The antigen specificity of nasal IgD was investigated by using ELISA. Results: The mRNA expression of immunoglobulin heavy constant delta gene, numbers of IgD+ cells, and protein levels of secretory IgD in sinonasal mucosa were increased in patients with CRS with or without nasal polyps compared with control subjects. Numbers of IgD+ plasmablasts were increased in both eosinophilic and noneosinophilic polyps, whereas numbers of IgD+ mast cells were only increased in eosinophilic polyps. Cross-linking IgD induced serum preincubated HMC-1 cells and polyp mast cells to produce B-cell activating factor, IL-21, IL-4, and IL-13 and to promote IgM, IgG, IgA, and IgE production from B cells. In eosinophilic polyps expression of those B cell–stimulating factors in mast cells and close contact between mast cells and B cells were found. Moreover, positive correlations of total IgD levels with total IgE levels and eosinophilia and upregulation of specific IgD against house dust mites were discovered in eosinophilic polyps. Conclusion: IgD-activated mast cells can facilitate IgE production and eosinophilic inflammation in patients with CRS with nasal polyps.",
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AU - Zhai, Guan Ting

AU - Wang, Hai

AU - Li, Jing Xian

AU - Cao, Ping Ping

AU - Jiang, Wen Xiu

AU - Song, Jia

AU - Yao, Yin

AU - Wang, Zhi Chao

AU - Wang, Zhe Zheng

AU - Wang, Meng Chen

AU - Liao, Bo

AU - Feng, Qi Miao

AU - Lu, Xiang

AU - Wang, Heng

AU - Gao, Peisong

AU - Liu, Zheng

PY - 2018/11/1

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N2 - Background: Although upregulated expression of local IgD has been reported in patients with chronic rhinosinusitis (CRS), its function is unclear. Objective: We sought to explore the expression and function of soluble IgD in patients with CRS, particularly CRS with nasal polyps. Methods: IgD levels in sinonasal mucosa were analyzed by using RT-PCR and ELISA. Numbers and phenotypes of IgD+ cells were studied by means of immunohistochemistry, immunofluorescence, and flow cytometry. HMC-1 cells, a human mast cell line, and mast cells purified from eosinophilic polyps were cultured alone or with naive B cells purified from peripheral blood. The antigen specificity of nasal IgD was investigated by using ELISA. Results: The mRNA expression of immunoglobulin heavy constant delta gene, numbers of IgD+ cells, and protein levels of secretory IgD in sinonasal mucosa were increased in patients with CRS with or without nasal polyps compared with control subjects. Numbers of IgD+ plasmablasts were increased in both eosinophilic and noneosinophilic polyps, whereas numbers of IgD+ mast cells were only increased in eosinophilic polyps. Cross-linking IgD induced serum preincubated HMC-1 cells and polyp mast cells to produce B-cell activating factor, IL-21, IL-4, and IL-13 and to promote IgM, IgG, IgA, and IgE production from B cells. In eosinophilic polyps expression of those B cell–stimulating factors in mast cells and close contact between mast cells and B cells were found. Moreover, positive correlations of total IgD levels with total IgE levels and eosinophilia and upregulation of specific IgD against house dust mites were discovered in eosinophilic polyps. Conclusion: IgD-activated mast cells can facilitate IgE production and eosinophilic inflammation in patients with CRS with nasal polyps.

AB - Background: Although upregulated expression of local IgD has been reported in patients with chronic rhinosinusitis (CRS), its function is unclear. Objective: We sought to explore the expression and function of soluble IgD in patients with CRS, particularly CRS with nasal polyps. Methods: IgD levels in sinonasal mucosa were analyzed by using RT-PCR and ELISA. Numbers and phenotypes of IgD+ cells were studied by means of immunohistochemistry, immunofluorescence, and flow cytometry. HMC-1 cells, a human mast cell line, and mast cells purified from eosinophilic polyps were cultured alone or with naive B cells purified from peripheral blood. The antigen specificity of nasal IgD was investigated by using ELISA. Results: The mRNA expression of immunoglobulin heavy constant delta gene, numbers of IgD+ cells, and protein levels of secretory IgD in sinonasal mucosa were increased in patients with CRS with or without nasal polyps compared with control subjects. Numbers of IgD+ plasmablasts were increased in both eosinophilic and noneosinophilic polyps, whereas numbers of IgD+ mast cells were only increased in eosinophilic polyps. Cross-linking IgD induced serum preincubated HMC-1 cells and polyp mast cells to produce B-cell activating factor, IL-21, IL-4, and IL-13 and to promote IgM, IgG, IgA, and IgE production from B cells. In eosinophilic polyps expression of those B cell–stimulating factors in mast cells and close contact between mast cells and B cells were found. Moreover, positive correlations of total IgD levels with total IgE levels and eosinophilia and upregulation of specific IgD against house dust mites were discovered in eosinophilic polyps. Conclusion: IgD-activated mast cells can facilitate IgE production and eosinophilic inflammation in patients with CRS with nasal polyps.

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KW - chronic rhinosinusitis

KW - eosinophil

KW - IgD

KW - IgE

KW - mast cell

KW - nasal polyps

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