Identification of unique murine tumor associated antigens by tumor infiltrating lymphocytes using tumor specific secretion of interferon-γ and tumor necrosis factor

Richard J. Barth; James J. Mulé; Anthony L. Asher; Martin G. Sanda; Steven A. Rosenberg

doi:10.1016/0022-1759(91)90380-X

Identification of unique murine tumor associated antigens by tumor infiltrating lymphocytes using tumor specific secretion of interferon-γ and tumor necrosis factor

Richard J. Barth, James J. Mulé, Anthony L. Asher, Martin G. Sanda, Steven A. Rosenberg

School of Medicine

Research output: Contribution to journal › Article › peer-review

Abstract

Stimulation of multiple CD8⁺ murine tumor infiltrating lymphocyte (TIL) lines and one TIL clone with the tumor of origin of the TIL induced at least three-fold more secretion of TNF and/or INF-γ than was elicited by other syngeneic, methylcholanthrene (MCA) induced sarcomas. TIL which specifically secreted lymphokines were generated from three different sarcomas. Specific lymphokine secretion was a stable characteristic of the lines over time. IL-2 was necessary for maximal lymphokine secretion by TIL. These investigations demonstrate that lymphokine secretion by CD8⁺ lymphocytes derived from tumor bearing mice can be used to define unique tumor associated antigens on at least three different sarcomas and may be valuable in studies of the biologic nature of these antigens and of the adoptive immunotherapy of cancer.

Original language	English (US)
Pages (from-to)	269-279
Number of pages	11
Journal	Journal of Immunological Methods
Volume	140
Issue number	2
DOIs	https://doi.org/10.1016/0022-1759(91)90380-X
State	Published - 1991

Keywords

Adoptive immunotherapy
Interferon-γ
Lymphokine secretion
Tumor antigens
Tumor infiltrating lymphocyte
Tumor necrosis factor

ASJC Scopus subject areas

Immunology and Allergy
Immunology

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10.1016/0022-1759(91)90380-X

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Identification of unique murine tumor associated antigens by tumor infiltrating lymphocytes using tumor specific secretion of interferon-γ and tumor necrosis factor. / Barth, Richard J.; Mulé, James J.; Asher, Anthony L.; Sanda, Martin G.; Rosenberg, Steven A.

In: Journal of Immunological Methods, Vol. 140, No. 2, 1991, p. 269-279.

Research output: Contribution to journal › Article › peer-review

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title = "Identification of unique murine tumor associated antigens by tumor infiltrating lymphocytes using tumor specific secretion of interferon-γ and tumor necrosis factor",

abstract = "Stimulation of multiple CD8+ murine tumor infiltrating lymphocyte (TIL) lines and one TIL clone with the tumor of origin of the TIL induced at least three-fold more secretion of TNF and/or INF-γ than was elicited by other syngeneic, methylcholanthrene (MCA) induced sarcomas. TIL which specifically secreted lymphokines were generated from three different sarcomas. Specific lymphokine secretion was a stable characteristic of the lines over time. IL-2 was necessary for maximal lymphokine secretion by TIL. These investigations demonstrate that lymphokine secretion by CD8+ lymphocytes derived from tumor bearing mice can be used to define unique tumor associated antigens on at least three different sarcomas and may be valuable in studies of the biologic nature of these antigens and of the adoptive immunotherapy of cancer.",

keywords = "Adoptive immunotherapy, Interferon-γ, Lymphokine secretion, Tumor antigens, Tumor infiltrating lymphocyte, Tumor necrosis factor",

author = "Barth, {Richard J.} and Mul{\'e}, {James J.} and Asher, {Anthony L.} and Sanda, {Martin G.} and Rosenberg, {Steven A.}",

year = "1991",

doi = "10.1016/0022-1759(91)90380-X",

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AU - Barth, Richard J.

AU - Mulé, James J.

AU - Asher, Anthony L.

AU - Sanda, Martin G.

AU - Rosenberg, Steven A.

PY - 1991

Y1 - 1991

N2 - Stimulation of multiple CD8+ murine tumor infiltrating lymphocyte (TIL) lines and one TIL clone with the tumor of origin of the TIL induced at least three-fold more secretion of TNF and/or INF-γ than was elicited by other syngeneic, methylcholanthrene (MCA) induced sarcomas. TIL which specifically secreted lymphokines were generated from three different sarcomas. Specific lymphokine secretion was a stable characteristic of the lines over time. IL-2 was necessary for maximal lymphokine secretion by TIL. These investigations demonstrate that lymphokine secretion by CD8+ lymphocytes derived from tumor bearing mice can be used to define unique tumor associated antigens on at least three different sarcomas and may be valuable in studies of the biologic nature of these antigens and of the adoptive immunotherapy of cancer.

AB - Stimulation of multiple CD8+ murine tumor infiltrating lymphocyte (TIL) lines and one TIL clone with the tumor of origin of the TIL induced at least three-fold more secretion of TNF and/or INF-γ than was elicited by other syngeneic, methylcholanthrene (MCA) induced sarcomas. TIL which specifically secreted lymphokines were generated from three different sarcomas. Specific lymphokine secretion was a stable characteristic of the lines over time. IL-2 was necessary for maximal lymphokine secretion by TIL. These investigations demonstrate that lymphokine secretion by CD8+ lymphocytes derived from tumor bearing mice can be used to define unique tumor associated antigens on at least three different sarcomas and may be valuable in studies of the biologic nature of these antigens and of the adoptive immunotherapy of cancer.

KW - Adoptive immunotherapy

KW - Interferon-γ

KW - Lymphokine secretion

KW - Tumor antigens

KW - Tumor infiltrating lymphocyte

KW - Tumor necrosis factor

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Identification of unique murine tumor associated antigens by tumor infiltrating lymphocytes using tumor specific secretion of interferon-γ and tumor necrosis factor

Abstract

Keywords

ASJC Scopus subject areas

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