Identification of unique genetic markers in Rv0927c among Mycobacterium tuberculosis W-Beijing strains

Xin Jiang, Ying Zhang, Wenhong Zhang, Feng Gao, Jialin Jin, Xuelian Zhang, Chong Lv, Qian Gao, Jing Chen, Honghai Wang

Research output: Contribution to journalArticle

Abstract

Fifty-six clinical isolates of Mycobacterium tuberculosis were analyzed by spoligotyping to determine the prevalence of W-Beijing strains. Forty-nine of the 56 isolates belonged to W-Beijing strains and 7 isolates were non-Beijing strains. Comparative two-dimensional gel electrophoresis analysis of protein patterns between the W-Beijing and non-Beijing strains identified a unique protein Rv0927c that is absent in the former but present in the latter and the reference strain M. tuberculosis H37Rv. Compared with 7 non-Beijing clinical isolates and H37Rv, all 49 W-Beijing strains had two characteristic mutations, a deletion of AGC at nucleotide position 421 of Rv0927c gene encoding a putative short dehydrogenase/reductase, causing deletion of serine codon at amino acid position 141 and a -127 G→A mutation in Rv0927c-pstS3 intergenic region, resulting in failure to express Rv0927c. Western blot analysis indicated that polyclonal antibody raised against H37Rv Rv0927c overexpressed in Escherichia coli reacted with non-Beijing strains and H37Rv but not W-Beijing strains. Characteristic mutations of Rv0927c that are present in W-Beijing strains can be used as a novel genetic marker for rapid molecular typing of M. tuberculosis W-Beijing strains.

Original languageEnglish (US)
Pages (from-to)241-246
Number of pages6
JournalMicrobes and Infection
Volume9
Issue number2
DOIs
Publication statusPublished - Feb 2007

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Keywords

  • Genetic markers
  • Mutation
  • Mycobacterium tuberculosis
  • Rv0927c
  • Strain typing
  • W-Beijing strain

ASJC Scopus subject areas

  • Microbiology
  • Infectious Diseases

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