TY - JOUR
T1 - Identification of Ugandan HIV type 1 variants with unique patterns of recombination in pol involving subtypes A and D
AU - Eshleman, Susan H.
AU - Gonzales, Matthew J.
AU - Becker-Pergola, Graziella
AU - Cunningham, Shawn C.
AU - Guay, Laura A.
AU - Jackson, J. Brooks
AU - Shafer, Robert W.
PY - 2002
Y1 - 2002
N2 - Most HIV-1 infections in Uganda are caused by subtypes A and D. The prevalence of recombination and the sites of specific breakpoints between these subtypes have not been reported. HIV-1 pol sequences encoding protease (amino acids 1-99) and reverse transcriptase (amino acids 1-324) from 102 pregnant Ugandan women were analyzed by the Recombinant Identification Program, SimPlot, and examination of phylogenetically informative sites to identify sites of recombination between sequence segments belonging to different subtypes. Thirteen percent (13 of 102) of the pol sequences contained strong evidence of recombination between subtypes A and D. At least nine different patterns of recombination were observed. Five women infected with a recombinant virus transmitted the recombinant virus perinatally. In this population-based study, intersubtype recombinants were common. The large number of different types of pol recombinants identified suggests that recombination occurs readily in the pol region. Perinatal transmission of the recombinant viruses demonstrates their evolutionary stability.
AB - Most HIV-1 infections in Uganda are caused by subtypes A and D. The prevalence of recombination and the sites of specific breakpoints between these subtypes have not been reported. HIV-1 pol sequences encoding protease (amino acids 1-99) and reverse transcriptase (amino acids 1-324) from 102 pregnant Ugandan women were analyzed by the Recombinant Identification Program, SimPlot, and examination of phylogenetically informative sites to identify sites of recombination between sequence segments belonging to different subtypes. Thirteen percent (13 of 102) of the pol sequences contained strong evidence of recombination between subtypes A and D. At least nine different patterns of recombination were observed. Five women infected with a recombinant virus transmitted the recombinant virus perinatally. In this population-based study, intersubtype recombinants were common. The large number of different types of pol recombinants identified suggests that recombination occurs readily in the pol region. Perinatal transmission of the recombinant viruses demonstrates their evolutionary stability.
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U2 - 10.1089/088922202317406655
DO - 10.1089/088922202317406655
M3 - Article
C2 - 12015904
AN - SCOPUS:0036257226
SN - 0889-2229
VL - 18
SP - 507
EP - 511
JO - AIDS research and human retroviruses
JF - AIDS research and human retroviruses
IS - 7
ER -