Identification of tyrosine O Sulfated proteins in cow retina and the 661W cell line

Yogita Kanan, Muayyad R. Al-Ubaidi

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Lack of tyrosine O Sulfation compromises both rod and cone electroretinographic responses emphasizing the importance of this post-translational modification for vision. To identify tyrosine sulfated proteins in retina, cow retinal lysates were subjected to immunoaffinity purification using an anti-sulfotyrosine antibody. The tyrosine sulfated proteins were eluted from the column using a sulfotyrosine pentapeptide and identified using mass spectrometry. Similarly, tyrosine sulfated proteins secreted by the 661W cell line were identified. Proteins identified were vitronectin, fibronectin, fibulin 2, nidogen, collagen V alpha 2, complement component 3 and C4 and fibrinogen beta. All proteins were subjected to analysis by ‘Sulfinator’ to determine potential sulfated tyrosines.

Original languageEnglish (US)
Title of host publicationAdvances in Experimental Medicine and Biology
PublisherSpringer New York LLC
Pages649-654
Number of pages6
Volume854
DOIs
StatePublished - Oct 1 2016
Externally publishedYes

Publication series

NameAdvances in Experimental Medicine and Biology
Volume854
ISSN (Print)00652598
ISSN (Electronic)22148019

Fingerprint

Tyrosine
Retina
Cells
Cell Line
Proteins
Complement C4
Vitronectin
Vertebrate Photoreceptor Cells
Complement C3
Post Translational Protein Processing
Fibronectins
Fibrinogen
Purification
Mass spectrometry
Cones
Anti-Idiotypic Antibodies
Mass Spectrometry
Collagen
Antibodies
tyrosine O-sulfate

Keywords

  • 661W
  • Posttranslational modification
  • PSG2
  • Retina
  • Tyrosine sulfation

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Kanan, Y., & Al-Ubaidi, M. R. (2016). Identification of tyrosine O Sulfated proteins in cow retina and the 661W cell line. In Advances in Experimental Medicine and Biology (Vol. 854, pp. 649-654). (Advances in Experimental Medicine and Biology; Vol. 854). Springer New York LLC. https://doi.org/10.1007/978-3-319-17121-0_86

Identification of tyrosine O Sulfated proteins in cow retina and the 661W cell line. / Kanan, Yogita; Al-Ubaidi, Muayyad R.

Advances in Experimental Medicine and Biology. Vol. 854 Springer New York LLC, 2016. p. 649-654 (Advances in Experimental Medicine and Biology; Vol. 854).

Research output: Chapter in Book/Report/Conference proceedingChapter

Kanan, Y & Al-Ubaidi, MR 2016, Identification of tyrosine O Sulfated proteins in cow retina and the 661W cell line. in Advances in Experimental Medicine and Biology. vol. 854, Advances in Experimental Medicine and Biology, vol. 854, Springer New York LLC, pp. 649-654. https://doi.org/10.1007/978-3-319-17121-0_86
Kanan Y, Al-Ubaidi MR. Identification of tyrosine O Sulfated proteins in cow retina and the 661W cell line. In Advances in Experimental Medicine and Biology. Vol. 854. Springer New York LLC. 2016. p. 649-654. (Advances in Experimental Medicine and Biology). https://doi.org/10.1007/978-3-319-17121-0_86
Kanan, Yogita ; Al-Ubaidi, Muayyad R. / Identification of tyrosine O Sulfated proteins in cow retina and the 661W cell line. Advances in Experimental Medicine and Biology. Vol. 854 Springer New York LLC, 2016. pp. 649-654 (Advances in Experimental Medicine and Biology).
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