Identification of tyrosine O Sulfated proteins in cow retina and the 661W cell line

Yogita Kanan, Muayyad R. Al-Ubaidi

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Lack of tyrosine O Sulfation compromises both rod and cone electroretinographic responses emphasizing the importance of this post-translational modification for vision. To identify tyrosine sulfated proteins in retina, cow retinal lysates were subjected to immunoaffinity purification using an anti-sulfotyrosine antibody. The tyrosine sulfated proteins were eluted from the column using a sulfotyrosine pentapeptide and identified using mass spectrometry. Similarly, tyrosine sulfated proteins secreted by the 661W cell line were identified. Proteins identified were vitronectin, fibronectin, fibulin 2, nidogen, collagen V alpha 2, complement component 3 and C4 and fibrinogen beta. All proteins were subjected to analysis by ‘Sulfinator’ to determine potential sulfated tyrosines.

Original languageEnglish (US)
Title of host publicationAdvances in Experimental Medicine and Biology
PublisherSpringer New York LLC
Pages649-654
Number of pages6
DOIs
StatePublished - Oct 1 2016
Externally publishedYes

Publication series

NameAdvances in Experimental Medicine and Biology
Volume854
ISSN (Print)0065-2598
ISSN (Electronic)2214-8019

Keywords

  • 661W
  • PSG2
  • Posttranslational modification
  • Retina
  • Tyrosine sulfation

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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