TY - CHAP
T1 - Identification of tyrosine O Sulfated proteins in cow retina and the 661W cell line
AU - Kanan, Yogita
AU - Al-Ubaidi, Muayyad R.
PY - 2016/10/1
Y1 - 2016/10/1
N2 - Lack of tyrosine O Sulfation compromises both rod and cone electroretinographic responses emphasizing the importance of this post-translational modification for vision. To identify tyrosine sulfated proteins in retina, cow retinal lysates were subjected to immunoaffinity purification using an anti-sulfotyrosine antibody. The tyrosine sulfated proteins were eluted from the column using a sulfotyrosine pentapeptide and identified using mass spectrometry. Similarly, tyrosine sulfated proteins secreted by the 661W cell line were identified. Proteins identified were vitronectin, fibronectin, fibulin 2, nidogen, collagen V alpha 2, complement component 3 and C4 and fibrinogen beta. All proteins were subjected to analysis by ‘Sulfinator’ to determine potential sulfated tyrosines.
AB - Lack of tyrosine O Sulfation compromises both rod and cone electroretinographic responses emphasizing the importance of this post-translational modification for vision. To identify tyrosine sulfated proteins in retina, cow retinal lysates were subjected to immunoaffinity purification using an anti-sulfotyrosine antibody. The tyrosine sulfated proteins were eluted from the column using a sulfotyrosine pentapeptide and identified using mass spectrometry. Similarly, tyrosine sulfated proteins secreted by the 661W cell line were identified. Proteins identified were vitronectin, fibronectin, fibulin 2, nidogen, collagen V alpha 2, complement component 3 and C4 and fibrinogen beta. All proteins were subjected to analysis by ‘Sulfinator’ to determine potential sulfated tyrosines.
KW - 661W
KW - PSG2
KW - Posttranslational modification
KW - Retina
KW - Tyrosine sulfation
UR - http://www.scopus.com/inward/record.url?scp=84943339775&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84943339775&partnerID=8YFLogxK
U2 - 10.1007/978-3-319-17121-0_86
DO - 10.1007/978-3-319-17121-0_86
M3 - Chapter
C2 - 26427471
AN - SCOPUS:84943339775
T3 - Advances in Experimental Medicine and Biology
SP - 649
EP - 654
BT - Advances in Experimental Medicine and Biology
PB - Springer New York LLC
ER -