Identification of two types of specific endothelin receptors in rat mesangial cell

Masanori Sugiura, Rudolf M. Snajdar, Mindy Schwartzberg, Kamal F. Badr, Tadashi Inagami

Research output: Contribution to journalArticlepeer-review

Abstract

Two types of receptors specific for endothelin were identified using cross-linking technique in cultured rat mesangial cells. The molecular weights of these receptors were approximately 58,000 and 34,000 by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate. The binding of radio-iodinated-endothelin to its receptors was inhibited by excess of unlabeled endothelin, but not by nifedipine, nicardipine, verapamil, diltiazem, angiotensin II or [Arg8]-vasopressin. The endothelin binding proteins were solubilized with 1% digitonin and fractionated under non-denaturing conditions by gel filtration. Two endothelin binding peaks eluted at the positions corresponding to the molecular weights of 65,000 and 43,000. These observations indicate that there are two types of specific endothelin receptors in rat mesangial cells which are distinct from voltage dependent L-type calcium channel.

Original languageEnglish (US)
Pages (from-to)1396-1401
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume162
Issue number3
DOIs
StatePublished - Aug 15 1989
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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