Identification of two species of yeast-like symbiotes in the brown planthopper, Nilaparvata lugens

Shengzhang Dong, Kun Pang, Xu Bai, Xiaoping Yu, Peiying Hao

Research output: Contribution to journalArticle

Abstract

To determine the species of the yeast-like symbionts (YLS) in the brown planthoppers (BPH), Nilaparvata lugens, YLS were first isolated and purified by ultracentrifugation from the fat bodies of BPH, and then 18S rDNA and internal transcribed spacer (ITS)-5.8S rDNA sequences of YLS were amplified with the different general primers for fungi. The results showed that the two different 18S and ITS-5.8S rDNA sequences of YLS were obtained. One 2291-bp DNA sequence, which contained 18S and ITS-5.8S rDNA, showed the high similarity to Cryptococcus and was named Cryp-Like symbiotes. Another 1248-bp DNA sequence, which contained a part of 18S and ITS-5.8S rDNA, showed the high similarity to Pichia guilliermondii and was named Pichia-Like symbiotes. It was further proved that Cryp- and Pichia-Like symbiotes existed in BPH through nested PCR with specific primers for two symbiotes and in situ hybridization analysis using digoxigenin-labeled probes. Our results showed that BPH harbored more than one species of eukaryotic YLS, which suggested that diversity of fungal endosymbiotes may be occurred in planthoppers, just like bacterial endosymbiotes.

Original languageEnglish (US)
Pages (from-to)1133-1138
Number of pages6
JournalCurrent Microbiology
Volume62
Issue number4
DOIs
StatePublished - Apr 1 2011
Externally publishedYes

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Ribosomal DNA
Yeasts
Pichia
Cryptococcus
Digoxigenin
Fat Body
Ultracentrifugation
In Situ Hybridization
Fungi
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Microbiology
  • Applied Microbiology and Biotechnology

Cite this

Identification of two species of yeast-like symbiotes in the brown planthopper, Nilaparvata lugens. / Dong, Shengzhang; Pang, Kun; Bai, Xu; Yu, Xiaoping; Hao, Peiying.

In: Current Microbiology, Vol. 62, No. 4, 01.04.2011, p. 1133-1138.

Research output: Contribution to journalArticle

Dong, Shengzhang ; Pang, Kun ; Bai, Xu ; Yu, Xiaoping ; Hao, Peiying. / Identification of two species of yeast-like symbiotes in the brown planthopper, Nilaparvata lugens. In: Current Microbiology. 2011 ; Vol. 62, No. 4. pp. 1133-1138.
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