Identification of tumor-suppressor genes using human melanoma cell lines UACC903, UACC903(+6), and SRS3 by comparison of expression profiles

Yan A. Su, Michael L. Bittner, Yidong Chen, Lian Tao, Yuan Jiang, Yinghua Zhang, Dietrich A. Stephan, Jeffrey M. Trent

Research output: Contribution to journalArticle

Abstract

The development and progression of cancer are believed to be due to multiple genetic alterations resulting in complex changes in expression of many genes. The parental malignant melanoma cell line UACC903 displays anchorage-independent growth, and the chromosome 6-suppressed subline UACC903(+6) displays anchorage-dependent growth. The anchorage-independent revertant cell line SRS3 derived from UACC903(+6) by retroviral transduction resembles the phenotype of UACC903. In this study, we first compared the expression profiles of 3317 genes between these three cell lines in pairs by cDNA microarrays, resulting in identification of genes with known suppressor activities. We then demonstrated connexin 43 (Cx43)-suppressing anchorage- independent growth of UACC903 on overexpression. Of 3317 genes with informative expression detected by cDNA microarray, 321 (9.68%) showed expression changes between at least one pair of the three cell lines. Notably, 12 genes displayed higher levels of expression in UACC903(+6) than in both UACC903 and SRS3, providing candidates for further identification of melanoma-suppressor genes. Genes encoding Cx43 (suppressor activity), monocyte chemotactic protein 1 (suppressor activity), and cysteine proteinase P32α (apoptotic activity) were all upregulated in UACC903(+6), in contrast to both UACC903 and SRS3. Transfection of Cx43, encoded on human chromosome 6q21 -q23, a region frequently altered in malignant melanoma, resulted in its overexpression and the suppression of anchorage-independent growth of UACC903. Thus, our result proves the principle that the combination of the ability to alter cellular phenotype by successive genetic alterations and the ability to examine the global expression profiles facilitates the identification of tumor suppressor genes. (C) 2000 Wiley-Liss, Inc.

Original languageEnglish (US)
Pages (from-to)119-127
Number of pages9
JournalMolecular Carcinogenesis
Volume28
Issue number2
DOIs
StatePublished - 2000
Externally publishedYes

Fingerprint

Tumor Suppressor Genes
Connexin 43
Melanoma
Cell Line
Growth
Oligonucleotide Array Sequence Analysis
Genes
Suppressor Genes
Phenotype
Chromosomes, Human, Pair 6
Cysteine Proteases
Chemokine CCL2
Human Chromosomes
Transcriptome
Transfection
Gene Expression
Neoplasms

Keywords

  • Anchorage-independent growth
  • cDNA microarray
  • Chromosome 6
  • Connexin 43
  • Gene expression profile

ASJC Scopus subject areas

  • Cancer Research
  • Molecular Biology

Cite this

Identification of tumor-suppressor genes using human melanoma cell lines UACC903, UACC903(+6), and SRS3 by comparison of expression profiles. / Su, Yan A.; Bittner, Michael L.; Chen, Yidong; Tao, Lian; Jiang, Yuan; Zhang, Yinghua; Stephan, Dietrich A.; Trent, Jeffrey M.

In: Molecular Carcinogenesis, Vol. 28, No. 2, 2000, p. 119-127.

Research output: Contribution to journalArticle

Su, Yan A. ; Bittner, Michael L. ; Chen, Yidong ; Tao, Lian ; Jiang, Yuan ; Zhang, Yinghua ; Stephan, Dietrich A. ; Trent, Jeffrey M. / Identification of tumor-suppressor genes using human melanoma cell lines UACC903, UACC903(+6), and SRS3 by comparison of expression profiles. In: Molecular Carcinogenesis. 2000 ; Vol. 28, No. 2. pp. 119-127.
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abstract = "The development and progression of cancer are believed to be due to multiple genetic alterations resulting in complex changes in expression of many genes. The parental malignant melanoma cell line UACC903 displays anchorage-independent growth, and the chromosome 6-suppressed subline UACC903(+6) displays anchorage-dependent growth. The anchorage-independent revertant cell line SRS3 derived from UACC903(+6) by retroviral transduction resembles the phenotype of UACC903. In this study, we first compared the expression profiles of 3317 genes between these three cell lines in pairs by cDNA microarrays, resulting in identification of genes with known suppressor activities. We then demonstrated connexin 43 (Cx43)-suppressing anchorage- independent growth of UACC903 on overexpression. Of 3317 genes with informative expression detected by cDNA microarray, 321 (9.68{\%}) showed expression changes between at least one pair of the three cell lines. Notably, 12 genes displayed higher levels of expression in UACC903(+6) than in both UACC903 and SRS3, providing candidates for further identification of melanoma-suppressor genes. Genes encoding Cx43 (suppressor activity), monocyte chemotactic protein 1 (suppressor activity), and cysteine proteinase P32α (apoptotic activity) were all upregulated in UACC903(+6), in contrast to both UACC903 and SRS3. Transfection of Cx43, encoded on human chromosome 6q21 -q23, a region frequently altered in malignant melanoma, resulted in its overexpression and the suppression of anchorage-independent growth of UACC903. Thus, our result proves the principle that the combination of the ability to alter cellular phenotype by successive genetic alterations and the ability to examine the global expression profiles facilitates the identification of tumor suppressor genes. (C) 2000 Wiley-Liss, Inc.",
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T1 - Identification of tumor-suppressor genes using human melanoma cell lines UACC903, UACC903(+6), and SRS3 by comparison of expression profiles

AU - Su, Yan A.

AU - Bittner, Michael L.

AU - Chen, Yidong

AU - Tao, Lian

AU - Jiang, Yuan

AU - Zhang, Yinghua

AU - Stephan, Dietrich A.

AU - Trent, Jeffrey M.

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AB - The development and progression of cancer are believed to be due to multiple genetic alterations resulting in complex changes in expression of many genes. The parental malignant melanoma cell line UACC903 displays anchorage-independent growth, and the chromosome 6-suppressed subline UACC903(+6) displays anchorage-dependent growth. The anchorage-independent revertant cell line SRS3 derived from UACC903(+6) by retroviral transduction resembles the phenotype of UACC903. In this study, we first compared the expression profiles of 3317 genes between these three cell lines in pairs by cDNA microarrays, resulting in identification of genes with known suppressor activities. We then demonstrated connexin 43 (Cx43)-suppressing anchorage- independent growth of UACC903 on overexpression. Of 3317 genes with informative expression detected by cDNA microarray, 321 (9.68%) showed expression changes between at least one pair of the three cell lines. Notably, 12 genes displayed higher levels of expression in UACC903(+6) than in both UACC903 and SRS3, providing candidates for further identification of melanoma-suppressor genes. Genes encoding Cx43 (suppressor activity), monocyte chemotactic protein 1 (suppressor activity), and cysteine proteinase P32α (apoptotic activity) were all upregulated in UACC903(+6), in contrast to both UACC903 and SRS3. Transfection of Cx43, encoded on human chromosome 6q21 -q23, a region frequently altered in malignant melanoma, resulted in its overexpression and the suppression of anchorage-independent growth of UACC903. Thus, our result proves the principle that the combination of the ability to alter cellular phenotype by successive genetic alterations and the ability to examine the global expression profiles facilitates the identification of tumor suppressor genes. (C) 2000 Wiley-Liss, Inc.

KW - Anchorage-independent growth

KW - cDNA microarray

KW - Chromosome 6

KW - Connexin 43

KW - Gene expression profile

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