TY - JOUR
T1 - Identification of TSIX, encoding an RNA antisense to human XIST, reveals differences from its murine counterpart
T2 - Implications for X inactivation
AU - Migeon, Barbara R.
AU - Chowdhury, Ashis K.
AU - Dunston, Jennifer A.
AU - McIntosh, Iain
N1 - Funding Information:
We are grateful to Dr. John Gearhart for the human EBD cells. We thank Kimesha Hammett and Joyce Axelman, for cell cultures; Tonya Watkins, for assistance with PCR studies; Roxanne Ashworth, for her contributions in designing primers; and Cheron Jones, for DNA sequencing. We gratefully acknowledge Drs. Mohamed Khalifa, Mita Mukherjee, Kirby Smith, and Ethylin W. Jabs, for helpful suggestions about the manuscript. This work was supported by NIH grants HD05465 (to B.R.M.) and AR44702 (to I.M.). J.A.D. is a student in the predoctoral program in human genetics, supported by National Institutes of Health training grant GM07814.
PY - 2001
Y1 - 2001
N2 - X inactivation is the mammalian method for X-chromosome dosage compensation, but some features of this developmental process vary among mammals. Such species variations provide insights into the essential components of the pathway. Tsix encodes a transcript antisense to the murine Xist transcript and is expressed in the mouse embryo only during the initial stages of X inactivation; it has been shown to play a role in imprinted X inactivation in the mouse placenta. We have identified its counterpart within the human X inactivation center (XIC). Human TSIX produces a >30-kb transcript that is expressed only in cells of fetal origin; it is expressed from human XIC transgenes in mouse embryonic stem cells and from human embryoid-body-derived cells, but not from human adult somatic cells. Differences in the structure of human and murine genes indicate that human TSIX was truncated during evolution. These differences could explain the fact that X inactivation is not imprinted in human placenta, and they raise questions about the role of TSIX in random X inactivation.
AB - X inactivation is the mammalian method for X-chromosome dosage compensation, but some features of this developmental process vary among mammals. Such species variations provide insights into the essential components of the pathway. Tsix encodes a transcript antisense to the murine Xist transcript and is expressed in the mouse embryo only during the initial stages of X inactivation; it has been shown to play a role in imprinted X inactivation in the mouse placenta. We have identified its counterpart within the human X inactivation center (XIC). Human TSIX produces a >30-kb transcript that is expressed only in cells of fetal origin; it is expressed from human XIC transgenes in mouse embryonic stem cells and from human embryoid-body-derived cells, but not from human adult somatic cells. Differences in the structure of human and murine genes indicate that human TSIX was truncated during evolution. These differences could explain the fact that X inactivation is not imprinted in human placenta, and they raise questions about the role of TSIX in random X inactivation.
UR - http://www.scopus.com/inward/record.url?scp=0034757204&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0034757204&partnerID=8YFLogxK
U2 - 10.1086/324022
DO - 10.1086/324022
M3 - Article
C2 - 11555794
AN - SCOPUS:0034757204
VL - 69
SP - 951
EP - 960
JO - American Journal of Human Genetics
JF - American Journal of Human Genetics
SN - 0002-9297
IS - 5
ER -