Identification of toxigenic Clostridium botulinum type E by enzyme immunoassay

Manoucher Dezfulian, John G. Bartlett

Research output: Contribution to journalArticle

Abstract

A sensitive double-sandwich enzyme immunoassay for identification of toxigenic Clostridium botulinum type E was developed with a type-E-specific antitoxin. The antitoxin was produced in a rabbit, following inoculation of minute quantities of type-E toxoid. The toxoid was prepared from a commercially available toxin by using a detoxification method. Cross-reactivity of the antitoxin with C. botulinum types A and B was avoided by absorption of the serum with alum-precipitated toxoids A and B. The immunoassay proved to be specific for C. botulinum type E because it did not react with culture filtrates of various Clostridium species, including nontoxigenenic "E-like" organisms and Clostridium sporogenes.

Original languageEnglish (US)
Pages (from-to)11-15
Number of pages5
JournalDiagnostic Microbiology and Infectious Disease
Volume14
Issue number1
DOIs
StatePublished - Jan 1 1991

    Fingerprint

ASJC Scopus subject areas

  • Microbiology (medical)
  • Infectious Diseases

Cite this