TY - JOUR
T1 - Identification of toxigenic Clostridium botulinum type E by enzyme immunoassay
AU - Dezfulian, Manoucher
AU - Bartlett, John G.
PY - 1991/1/1
Y1 - 1991/1/1
N2 - A sensitive double-sandwich enzyme immunoassay for identification of toxigenic Clostridium botulinum type E was developed with a type-E-specific antitoxin. The antitoxin was produced in a rabbit, following inoculation of minute quantities of type-E toxoid. The toxoid was prepared from a commercially available toxin by using a detoxification method. Cross-reactivity of the antitoxin with C. botulinum types A and B was avoided by absorption of the serum with alum-precipitated toxoids A and B. The immunoassay proved to be specific for C. botulinum type E because it did not react with culture filtrates of various Clostridium species, including nontoxigenenic "E-like" organisms and Clostridium sporogenes.
AB - A sensitive double-sandwich enzyme immunoassay for identification of toxigenic Clostridium botulinum type E was developed with a type-E-specific antitoxin. The antitoxin was produced in a rabbit, following inoculation of minute quantities of type-E toxoid. The toxoid was prepared from a commercially available toxin by using a detoxification method. Cross-reactivity of the antitoxin with C. botulinum types A and B was avoided by absorption of the serum with alum-precipitated toxoids A and B. The immunoassay proved to be specific for C. botulinum type E because it did not react with culture filtrates of various Clostridium species, including nontoxigenenic "E-like" organisms and Clostridium sporogenes.
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U2 - 10.1016/0732-8893(91)90080-Y
DO - 10.1016/0732-8893(91)90080-Y
M3 - Article
C2 - 2013205
AN - SCOPUS:0025968393
VL - 14
SP - 11
EP - 15
JO - Diagnostic Microbiology and Infectious Disease
JF - Diagnostic Microbiology and Infectious Disease
SN - 0732-8893
IS - 1
ER -