Identification of tissue-specific microRNA resonse in mice following exposure to energetic protons

Muhammad Akram Tariq, Christopher Brumbaugh, Shahid Khan, Honglu Wu, Govindarajan T. Ramesh, Nader Pourmand, Olufisayo Abimbola Jejelowo

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The understanding of cellular responses to proton exposure is of primary importance for the assessment and management of human health risks during space exploration missions. MicroRNAs (miRNAs) are small, non-coding RNAs that are widely involved in gene expression for multiple intracellular processes, including gene expressions made in response to cellular stress. The level of involvement of gene regulation through miRNA intervention in proton irradiated cells or animals are unknown. Balb/C male mice were exposed to charged particle radiation. Group 1 served as control receiving no radiation (0.0 Gy) and group 2 received 2.0 Gy from a proton source at a dose rate of 1Gev/45 seconds. The controls and irradiated mice were sacrificed by cervical decapitation four hours after radiation exposure. Following euthanization, testis tissues were dissected out and immediately frozen in liquid nitrogen. After passing through the quality control, 500ng of total RNA, isolated from testis tissues, was used in Illumina TruSeq small RNA sample prep kit to prepare libraries. Using Illumina HiSeq genome analyzer for miRNA sequencing, the levels of miRNAs in the testis tissues of irradiated mice (2Gy) relative to control mice (0Gy) were measured. Dysregulation of 25 different miRNA species, most of which are involved in cell growth, differentiation, survival and/or apoptosis was observed. Among the 25 differentially expressed miRNAs, a group of 10 miRNAs were up-regulated with mmu-miR-5100 being the most highly altered miRNA (8.1 fold change) and a group of 15 miRNAs were down-regulated with mmu-miR-142-3p being the down-regulated miRNA (-6.7 fold change). These results provide the first evidence that miRNA play a role in cellular defense against proton radiation. Additionally, the observed changes in miRNA expression are involved in very important signaling pathways such as Ras signaling and MAPK pathway, which may imply a significant health concern for astronauts. The National Aeronautics and Space Administration (NASA) Office of Education under the University Research Centers (URC) project, (Cooperative Agreements NNX08B47A and NNX10AQ16A), provided funding. National Institutes of Health (P01-HG000205) supported CB and SYK.

Original languageEnglish (US)
Title of host publication62nd International Astronautical Congress 2011, IAC 2011
Pages247-254
Number of pages8
StatePublished - Dec 1 2011
Externally publishedYes
Event62nd International Astronautical Congress 2011, IAC 2011 - Cape Town, South Africa
Duration: Oct 3 2011Oct 7 2011

Publication series

Name62nd International Astronautical Congress 2011, IAC 2011
Volume1

Conference

Conference62nd International Astronautical Congress 2011, IAC 2011
CountrySouth Africa
CityCape Town
Period10/3/1110/7/11

Fingerprint

Protons
RNA
Gene expression
Tissue
Radiation
Health
Health risks
Cell growth
Cell death
Liquid nitrogen
Charged particles
Quality control
NASA
Animals
Genes
Education
Cells

ASJC Scopus subject areas

  • Aerospace Engineering

Cite this

Tariq, M. A., Brumbaugh, C., Khan, S., Wu, H., Ramesh, G. T., Pourmand, N., & Jejelowo, O. A. (2011). Identification of tissue-specific microRNA resonse in mice following exposure to energetic protons. In 62nd International Astronautical Congress 2011, IAC 2011 (pp. 247-254). (62nd International Astronautical Congress 2011, IAC 2011; Vol. 1).

Identification of tissue-specific microRNA resonse in mice following exposure to energetic protons. / Tariq, Muhammad Akram; Brumbaugh, Christopher; Khan, Shahid; Wu, Honglu; Ramesh, Govindarajan T.; Pourmand, Nader; Jejelowo, Olufisayo Abimbola.

62nd International Astronautical Congress 2011, IAC 2011. 2011. p. 247-254 (62nd International Astronautical Congress 2011, IAC 2011; Vol. 1).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Tariq, MA, Brumbaugh, C, Khan, S, Wu, H, Ramesh, GT, Pourmand, N & Jejelowo, OA 2011, Identification of tissue-specific microRNA resonse in mice following exposure to energetic protons. in 62nd International Astronautical Congress 2011, IAC 2011. 62nd International Astronautical Congress 2011, IAC 2011, vol. 1, pp. 247-254, 62nd International Astronautical Congress 2011, IAC 2011, Cape Town, South Africa, 10/3/11.
Tariq MA, Brumbaugh C, Khan S, Wu H, Ramesh GT, Pourmand N et al. Identification of tissue-specific microRNA resonse in mice following exposure to energetic protons. In 62nd International Astronautical Congress 2011, IAC 2011. 2011. p. 247-254. (62nd International Astronautical Congress 2011, IAC 2011).
Tariq, Muhammad Akram ; Brumbaugh, Christopher ; Khan, Shahid ; Wu, Honglu ; Ramesh, Govindarajan T. ; Pourmand, Nader ; Jejelowo, Olufisayo Abimbola. / Identification of tissue-specific microRNA resonse in mice following exposure to energetic protons. 62nd International Astronautical Congress 2011, IAC 2011. 2011. pp. 247-254 (62nd International Astronautical Congress 2011, IAC 2011).
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