Identification of the Potential Key Long Non-coding RNAs in Aged Mice with Postoperative Cognitive Dysfunction

Ming Li, Chan Chen, Weiyi Zhang, Rui Gao, Qiao Wang, Hai Chen, Shu Zhang, Xiaobo Mao, Mathis Leblanc, Adam Behensky, Zheng Zhang, Lu Gan, Hai Yu, Tao Zhu, Jin Liu

Research output: Contribution to journalArticle

Abstract

Postoperative cognitive dysfunction (POCD) is a significant complication of surgery, particularly in elderly patients. Emerging researches showed that long non-coding RNA (lncRNA) may play a vital role in the pathogenesis of POCD. Here we aimed to identify potential key lncRNAs involved in the development of POCD. LncRNA and mRNA expression profiles in hippocampal tissues from POCD and control mice were analyzed by microarray assay. Gene ontology (GO) and KEGG pathway enrichment analyses were conducted to probe the functions of dysregulated genes. Then, important factors of the mainly affected biological processes were measured in the hippocampus. Correlated coding-non-coding co-expression (CNC) networks were constructed. Finally, the potential key pairs of lncRNA and target mRNA implicated in POCD were probed. Our data showed that 868 differentially expressed lncRNAs and 690 differentially expressed mRNAs were identified in total. GO and KEGG analyses indicated that the differentially expressed genes were mainly associated with inflammatory and apoptotic signaling pathways. Surgery-induced inflammatory cytokines and apoptosis were significantly increased in hippocampal tissues of aged mice. In CNC network analysis, we found that LncRNA uc009qbj.1 was positively correlated with apoptosis-associated gene Vrk2 level. LncRNA ENSMUST00000174338 correlated positively with expression of the inflammation and apoptosis-associated gene Smad7. LncRNA NONMMUT00000123687 mediated gene expression by binding the inflammation-regulated transcription factor Meis2. Our results suggested that these potential key lncRNAs and mRNAs may play a crucial role in the development of POCD through mediating neuronal inflammation or apoptosis.

Original languageEnglish (US)
Article number189
JournalFrontiers in Aging Neuroscience
Volume10
Issue numberJUL
DOIs
Publication statusPublished - Jan 1 2019

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Keywords

  • Aging
  • Apoptosis
  • Inflammation
  • Long non-coding RNA
  • Microarray
  • Postoperative cognitive dysfunction

ASJC Scopus subject areas

  • Aging
  • Cognitive Neuroscience

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