TY - JOUR
T1 - Identification of the physiological promoter for spinocerebellar ataxia 2 gene reveals a CpG island for promoter activity situated into the exon 1 of this gene and provides data about the origin of the nonmethylated state of these types of islands
AU - Aguiar, Jorge
AU - Santurlidis, Simon
AU - Nowok, Joachim
AU - Alexander, Christiane
AU - Rudnicki, Doda
AU - Gispert, Suzana
AU - Schulz, Wolfgang
AU - Auburger, Georg
N1 - Funding Information:
We gratefully thank Dr. Frank Bosse, for his excellent comments, and Dr. Britta Urmoneit for helping us obtain and maintain the neuroblastoma SH-SY5Y cell-line. We are also very grateful to Nicola Bergmann and Ingrid Prikulis for their excellent technical assistance. This work was supported by the Deutsche Forschungs-gemeinschaft and by the Deutsche Akademischer Austauschdienst (DAAD). In addition, we want to thank the Cuban Center for Genetic Engineering and Biotechnology (CIGB) for its understanding and collaboration in the completion of this work.
PY - 1999/1/19
Y1 - 1999/1/19
N2 - In order to further use the spinocerebellar ataxia 2 (SCA2) promoter for transgenic mice models of 'CAG repeat' neurodegeneration, different fragments of this 5' end were ligated into pGL3-Luc plasmid to obtain the better promoter-activity of the physiological promoter for SCA2. Base-par composition of the SCA2-5' region, and promoter prediction algorithms such as TSSW and TSSG, together with the high firefly luciferase expression after 48 hours of transient transfection in mammalian cells lines, showed a typical CpG island for promoter-activity. The promoter activity was specifically localized into the exon 1 of the SCA2 gene. The higher expression of firefly luciferase in the embryonal F9 cells by the use of SCA2 promoter, rather than by the use of CMV promoter may be related with the origin of the nonmethylated CpG island during the early embryogenesis. Analysis of the 5' region from HD gene revealed to a CpG island, which could be containing the physiological promoter for this gene.
AB - In order to further use the spinocerebellar ataxia 2 (SCA2) promoter for transgenic mice models of 'CAG repeat' neurodegeneration, different fragments of this 5' end were ligated into pGL3-Luc plasmid to obtain the better promoter-activity of the physiological promoter for SCA2. Base-par composition of the SCA2-5' region, and promoter prediction algorithms such as TSSW and TSSG, together with the high firefly luciferase expression after 48 hours of transient transfection in mammalian cells lines, showed a typical CpG island for promoter-activity. The promoter activity was specifically localized into the exon 1 of the SCA2 gene. The higher expression of firefly luciferase in the embryonal F9 cells by the use of SCA2 promoter, rather than by the use of CMV promoter may be related with the origin of the nonmethylated CpG island during the early embryogenesis. Analysis of the 5' region from HD gene revealed to a CpG island, which could be containing the physiological promoter for this gene.
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U2 - 10.1006/bbrc.1998.9929
DO - 10.1006/bbrc.1998.9929
M3 - Article
C2 - 9918835
AN - SCOPUS:0033582337
SN - 0006-291X
VL - 254
SP - 315
EP - 318
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 2
ER -