Identification of the physiological promoter for spinocerebellar ataxia 2 gene reveals a CpG island for promoter activity situated into the exon 1 of this gene and provides data about the origin of the nonmethylated state of these types of islands

Jorge Aguiar, Simon Santurlidis, Joachim Nowok, Christiane Alexander, Doda Rudnicki, Suzana Gispert, Wolfgang Schulz, Georg Auburger

Research output: Contribution to journalArticlepeer-review

Abstract

In order to further use the spinocerebellar ataxia 2 (SCA2) promoter for transgenic mice models of 'CAG repeat' neurodegeneration, different fragments of this 5' end were ligated into pGL3-Luc plasmid to obtain the better promoter-activity of the physiological promoter for SCA2. Base-par composition of the SCA2-5' region, and promoter prediction algorithms such as TSSW and TSSG, together with the high firefly luciferase expression after 48 hours of transient transfection in mammalian cells lines, showed a typical CpG island for promoter-activity. The promoter activity was specifically localized into the exon 1 of the SCA2 gene. The higher expression of firefly luciferase in the embryonal F9 cells by the use of SCA2 promoter, rather than by the use of CMV promoter may be related with the origin of the nonmethylated CpG island during the early embryogenesis. Analysis of the 5' region from HD gene revealed to a CpG island, which could be containing the physiological promoter for this gene.

Original languageEnglish (US)
Pages (from-to)315-318
Number of pages4
JournalBiochemical and Biophysical Research Communications
Volume254
Issue number2
DOIs
StatePublished - Jan 19 1999

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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