Identification of testis-specific male contraceptive targets: Insights from transcriptional profiling of the cycle of the rat seminiferous epithelium and purified testicular cells

Daniel S. Johnston, Scott A. Jelinsky, Yu Zhi, Joshua N. Finger, Gregory S. Kopf, William W Wright

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

In an effort to identify novel targets for the development of nonhormonal male contraceptives, genome-wide transcriptional profiling of the rat testis was performed. Specifically, enzymatically purified spermatogonia plus early spermatocyctes, pachytene spermatocytes, round spermatids, and Sertoli cells was analyzed along with microdissected rat seminiferous tubules at stages I, II-III, IV-V, VI, VIIa,b, VIIc,d, VIII, IX- XI, XII, XIII-XIV of the cycle of the seminiferous epithelium using RAE 230_2.0 microarrays. The combined analysis of these studies identified 16,971 expressed probe sets on the array. How these expression data, combined with additional bioinformatic data analysis and quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) analysis, led to the identification of 58 genes that have 1000-fold higher expression transcriptionally in the testis when compared to over 20 other nonreproductive tissues is described. The products of these genes may play important roles in testicular and/or sperm function, and further investigation on their utility as nonhormonal contraceptive targets is warranted. Moreover, these microarray data have been used to expedite the identification of a mutation in RIKEN cDNA 2410004F06 gene as likely being responsible for spermatogenic failure in a line of infertile mice generated by N-ethyl-N-nitrosourea (ENU) mutagenesis. The microarray data and the qRT-PCR data described are available in the Mammalian Reproductive Genetics database (http://mrg.genetics.washington.edu/).

Original languageEnglish (US)
Title of host publicationAnnals of the New York Academy of Sciences
Pages36-46
Number of pages11
Volume1120
DOIs
StatePublished - Dec 2007

Publication series

NameAnnals of the New York Academy of Sciences
Volume1120
ISSN (Print)00778923
ISSN (Electronic)17496632

Fingerprint

Male Contraceptive Agents
Seminiferous Epithelium
Testis
Rats
Microarrays
Genes
Reverse Transcriptase Polymerase Chain Reaction
Polymerase chain reaction
RNA-Directed DNA Polymerase
Ethylnitrosourea
Genetic Databases
Spermatogonia
Seminiferous Tubules
Spermatocytes
Spermatids
Sertoli Cells
Contraceptive Agents
Computational Biology
Mutagenesis
Spermatozoa

Keywords

  • Contraception
  • Microarray
  • Seminferous epithelium
  • Testis
  • Testis-specific

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Johnston, D. S., Jelinsky, S. A., Zhi, Y., Finger, J. N., Kopf, G. S., & Wright, W. W. (2007). Identification of testis-specific male contraceptive targets: Insights from transcriptional profiling of the cycle of the rat seminiferous epithelium and purified testicular cells. In Annals of the New York Academy of Sciences (Vol. 1120, pp. 36-46). (Annals of the New York Academy of Sciences; Vol. 1120). https://doi.org/10.1196/annals.1411.014

Identification of testis-specific male contraceptive targets : Insights from transcriptional profiling of the cycle of the rat seminiferous epithelium and purified testicular cells. / Johnston, Daniel S.; Jelinsky, Scott A.; Zhi, Yu; Finger, Joshua N.; Kopf, Gregory S.; Wright, William W.

Annals of the New York Academy of Sciences. Vol. 1120 2007. p. 36-46 (Annals of the New York Academy of Sciences; Vol. 1120).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Johnston, DS, Jelinsky, SA, Zhi, Y, Finger, JN, Kopf, GS & Wright, WW 2007, Identification of testis-specific male contraceptive targets: Insights from transcriptional profiling of the cycle of the rat seminiferous epithelium and purified testicular cells. in Annals of the New York Academy of Sciences. vol. 1120, Annals of the New York Academy of Sciences, vol. 1120, pp. 36-46. https://doi.org/10.1196/annals.1411.014
Johnston DS, Jelinsky SA, Zhi Y, Finger JN, Kopf GS, Wright WW. Identification of testis-specific male contraceptive targets: Insights from transcriptional profiling of the cycle of the rat seminiferous epithelium and purified testicular cells. In Annals of the New York Academy of Sciences. Vol. 1120. 2007. p. 36-46. (Annals of the New York Academy of Sciences). https://doi.org/10.1196/annals.1411.014
Johnston, Daniel S. ; Jelinsky, Scott A. ; Zhi, Yu ; Finger, Joshua N. ; Kopf, Gregory S. ; Wright, William W. / Identification of testis-specific male contraceptive targets : Insights from transcriptional profiling of the cycle of the rat seminiferous epithelium and purified testicular cells. Annals of the New York Academy of Sciences. Vol. 1120 2007. pp. 36-46 (Annals of the New York Academy of Sciences).
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