TY - JOUR
T1 - Identification of TDE2 gene and its expression in non-small cell lung cancer
AU - Player, Audrey
AU - Gillespie, John
AU - Fujii, Takeshi
AU - Fukuoka, Junya
AU - Dracheva, Tatiana
AU - Meerzaman, Dauod
AU - Hong, Kyeong Man
AU - Curran, John
AU - Attoh, Goziam
AU - Travis, William
AU - Jen, Jin
PY - 2003/11/1
Y1 - 2003/11/1
N2 - TDE2, a gene with sequence similarity to the mouse testicular tumor-differentially-expressed (Tde1/MUSTETU) gene, was identified by serial analysis of gene expression (SAGE) in non-small cell lung cancers (NSCLC). Here we characterized the TDE2 gene and determined its transcript levels in a panel of lung tumors, adjacent nonmalignant lung tissues and a variety of normal human tissues. In addition, we show that TDE2 is a potential transmembrane protein with 11 putative transmembrane helices. Using real-time quantitative PCR, we showed that TDE2 transcript levels were higher in NSCLC compared to nonmalignant samples. In nonpulmonary normal tissues, the level of TDE2 was the highest in bladder, kidney and muscle; moderate to low in stomach, liver, skin, placenta and ovary tissues; and undetectable in brain, spleen and heart. By in situ hybridization, we showed that 10 of 18 lung tumors and only 1 of 14 adjacent nonmalignant regions had high levels of TDE2 transcripts. Alternatively, only 2 of 18 tumors and 8 of 14 adjacent nonmalignant bronchiole epithelium regions demonstrated negative to low levels of TDE2 signals.
AB - TDE2, a gene with sequence similarity to the mouse testicular tumor-differentially-expressed (Tde1/MUSTETU) gene, was identified by serial analysis of gene expression (SAGE) in non-small cell lung cancers (NSCLC). Here we characterized the TDE2 gene and determined its transcript levels in a panel of lung tumors, adjacent nonmalignant lung tissues and a variety of normal human tissues. In addition, we show that TDE2 is a potential transmembrane protein with 11 putative transmembrane helices. Using real-time quantitative PCR, we showed that TDE2 transcript levels were higher in NSCLC compared to nonmalignant samples. In nonpulmonary normal tissues, the level of TDE2 was the highest in bladder, kidney and muscle; moderate to low in stomach, liver, skin, placenta and ovary tissues; and undetectable in brain, spleen and heart. By in situ hybridization, we showed that 10 of 18 lung tumors and only 1 of 14 adjacent nonmalignant regions had high levels of TDE2 transcripts. Alternatively, only 2 of 18 tumors and 8 of 14 adjacent nonmalignant bronchiole epithelium regions demonstrated negative to low levels of TDE2 signals.
KW - In situ hybridization
KW - Non-small cell lung cancer
KW - Serial analysis of gene expression (SAGE)
KW - TDE1
KW - TDE2
KW - Tms1
KW - Tms2
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U2 - 10.1002/ijc.11391
DO - 10.1002/ijc.11391
M3 - Article
C2 - 12949800
AN - SCOPUS:0141634196
SN - 0020-7136
VL - 107
SP - 238
EP - 243
JO - International Journal of Cancer
JF - International Journal of Cancer
IS - 2
ER -