Abstract
The functional protein microarray is a powerful and versatile systems biology and proteomics tool that allows the rapid activity profiling of thousands of proteins in parallel. We have recently developed a human proteome array, the HuProt array, which includes ~80% of all the full-length proteins of the human proteome. In one recent application of the HuProt array, we identified numerous SUMO E3 ligase-dependent SUMOylation substrates. For many SUMO E3 ligases, only a small number of substrates have been identified and the target specificities of these ligases therefore remain poorly defined. In this protocol, we outline a method we developed using the HuProt array to screen the human proteome to identify novel SUMO E3 ligase substrates recognized by specific E3 ligases.
Original language | English (US) |
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Pages (from-to) | 455-463 |
Number of pages | 9 |
Journal | Methods in Molecular Biology |
Volume | 1295 |
DOIs | |
State | Published - 2015 |
Keywords
- E3 ligases
- Microarray
- Posttranslational modifications
- Proteomics
- SUMO
ASJC Scopus subject areas
- Molecular Biology
- Genetics