Identification of Spin Diffusion Pathways in Isotopically Labeled Biomolecules

Thomas R. Eykyn; Dominique Früh; Geoffrey Bodenhausen

doi:10.1006/jmre.1999.1736

Identification of Spin Diffusion Pathways in Isotopically Labeled Biomolecules

Thomas R. Eykyn, Dominique Früh, Geoffrey Bodenhausen

Research output: Contribution to journal › Editorial › peer-review

2 Scopus citations

Abstract

One-dimensional NOE experiments applicable to labeled macromolecules are presented which allow the manipulation of specific spin diffusion pathways and thus unambiguously identify clandestine spins through which the direct NOE is mediated. A treatment of spin diffusion using average Liouvillian theory is shown to describe adequately these phenomena. Experiments are carried out on an ¹⁵ N-labeled sample of human ubiquitin.

Original language	English (US)
Pages (from-to)	330-333
Number of pages	4
Journal	Journal of Magnetic Resonance
Volume	138
Issue number	2
DOIs	https://doi.org/10.1006/jmre.1999.1736
State	Published - Jun 1999
Externally published	Yes

Keywords

Average Liouvillian theory
Protein NMR
Selective NOE
Spin diffusion

ASJC Scopus subject areas

Biophysics
Biochemistry
Nuclear and High Energy Physics
Condensed Matter Physics

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10.1006/jmre.1999.1736

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title = "Identification of Spin Diffusion Pathways in Isotopically Labeled Biomolecules",

abstract = " One-dimensional NOE experiments applicable to labeled macromolecules are presented which allow the manipulation of specific spin diffusion pathways and thus unambiguously identify clandestine spins through which the direct NOE is mediated. A treatment of spin diffusion using average Liouvillian theory is shown to describe adequately these phenomena. Experiments are carried out on an 15 N-labeled sample of human ubiquitin.",

keywords = "Average Liouvillian theory, Protein NMR, Selective NOE, Spin diffusion",

author = "Eykyn, {Thomas R.} and Dominique Fr{\"u}h and Geoffrey Bodenhausen",

note = "Funding Information: This work was supported by the Fonds National de la Recherche Scienti-fique, by the Commission pour la Technologie et l{\textquoteright}Innovation of Switzerland, and by the Centre National de la Recherche Scientifique of France.",

year = "1999",

month = jun,

doi = "10.1006/jmre.1999.1736",

language = "English (US)",

volume = "138",

pages = "330--333",

journal = "Journal of Magnetic Resonance",

issn = "1090-7807",

publisher = "Academic Press Inc.",

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T1 - Identification of Spin Diffusion Pathways in Isotopically Labeled Biomolecules

AU - Eykyn, Thomas R.

AU - Früh, Dominique

AU - Bodenhausen, Geoffrey

N1 - Funding Information: This work was supported by the Fonds National de la Recherche Scienti-fique, by the Commission pour la Technologie et l’Innovation of Switzerland, and by the Centre National de la Recherche Scientifique of France.

PY - 1999/6

Y1 - 1999/6

N2 - One-dimensional NOE experiments applicable to labeled macromolecules are presented which allow the manipulation of specific spin diffusion pathways and thus unambiguously identify clandestine spins through which the direct NOE is mediated. A treatment of spin diffusion using average Liouvillian theory is shown to describe adequately these phenomena. Experiments are carried out on an 15 N-labeled sample of human ubiquitin.

AB - One-dimensional NOE experiments applicable to labeled macromolecules are presented which allow the manipulation of specific spin diffusion pathways and thus unambiguously identify clandestine spins through which the direct NOE is mediated. A treatment of spin diffusion using average Liouvillian theory is shown to describe adequately these phenomena. Experiments are carried out on an 15 N-labeled sample of human ubiquitin.

KW - Average Liouvillian theory

KW - Protein NMR

KW - Selective NOE

KW - Spin diffusion

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UR - http://www.scopus.com/inward/citedby.url?scp=0033144287&partnerID=8YFLogxK

U2 - 10.1006/jmre.1999.1736

DO - 10.1006/jmre.1999.1736

M3 - Editorial

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AN - SCOPUS:0033144287

SN - 1090-7807

VL - 138

SP - 330

EP - 333

JO - Journal of Magnetic Resonance

JF - Journal of Magnetic Resonance

IS - 2

ER -

Identification of Spin Diffusion Pathways in Isotopically Labeled Biomolecules

Abstract

Keywords

ASJC Scopus subject areas

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