Identification of serologic biomarkers for predicting microvascular invasion in hepatocellular carcinoma

Yuan Quan Yu, Liang Wang, Yun Jin, Jia Le Zhou, Yan Hua Geng, Xing Jin, Xiao Xiao Zhang, Jun Jie Yang, Cheng Ming Qian, Dong Er Zhou, Da Ren Liu, Shu You Peng, Yan Luo, Lei Zheng, Jiang Tao Li

Research output: Contribution to journalArticle

Abstract

Microvascular invasion (MVI) of hepatocellular carcinoma (HCC) is a major risk factor for early recurrence and poor survival after curative surgical therapies. However, MVI can only be diagnosed by pathological examination following resection. The aim of this study is to identify serologic biomarkers for predicting MVI preoperatively to help facilitate treatment decisions. We used the sero-proteomic approach to identify antigens that induce corresponding antibody responses either specifically in the serum from MVI (+) patients or from MVI (-) patients. Six antigens were subsequently identified as HSP 70, HSP 90, alpha-enolase (Eno-1), Annexin A2, glutathione synthetase and beta-actin by mass spectrometry. The antibodies titers in sera corresponding to four of these six antigens were measured by ELISA and compared between 35 MVI (+) patients and 26 MVI (-) patients. The titers of anti-HSP 70 antibodies were significantly higher in MVI (-) patients than those in MVI (+) patients; and the titers of anti-Eno-1 antibodies were significantly lower in MVI (-) patients than those in MVI (+) patients. The results were subjected to multivariate analysis together with other clinicopathologic factors, suggesting that antibodies against HSP 70 and Eno-1 in sera are potential biomarkers for predicting MVI in HCC prior to surgical resection. These biomarkers should be further investigated as potential therapeutic targets.

Original languageEnglish (US)
Pages (from-to)16362-16371
Number of pages10
JournalOncotarget
Volume7
Issue number13
DOIs
StatePublished - Mar 29 2016

Keywords

  • Biomarker
  • Diagnosis
  • Hepatocellular carcinoma
  • Microvascular invasion
  • Sero-proteomics

ASJC Scopus subject areas

  • Oncology

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  • Cite this

    Yu, Y. Q., Wang, L., Jin, Y., Zhou, J. L., Geng, Y. H., Jin, X., Zhang, X. X., Yang, J. J., Qian, C. M., Zhou, D. E., Liu, D. R., Peng, S. Y., Luo, Y., Zheng, L., & Li, J. T. (2016). Identification of serologic biomarkers for predicting microvascular invasion in hepatocellular carcinoma. Oncotarget, 7(13), 16362-16371. https://doi.org/10.18632/oncotarget.7649