Identification of RNA binding motif proteins essential for cardiovascular development

Samantha Maragh, Ronald A. Miller, Seneca L. Bessling, David M. McGaughey, Marja W. Wessels, Bianca De Graaf, Eric A. Stone, Aida M. Bertoli-Avella, John D. Gearhart, Shannon Fisher, Andrew S McCallion

Research output: Contribution to journalArticle

Abstract

Background: We recently identified Rbm24 as a novel gene expressed during mouse cardiac development. Due to its tightly restricted and persistent expression from formation of the cardiac crescent onwards and later in forming vasculature we posited it to be a key player in cardiogenesis with additional roles in vasculogenesis and angiogenesis. Results: To determine the role of this gene in cardiac development, we have identified its zebrafish orthologs (rbm24a and rbm24b), and functionally evaluated them during zebrafish embryogenesis. Consistent with our underlying hypothesis, reduction in expression of either ortholog through injection of morpholino antisense oligonucleotides results in cardiogenic defects including cardiac looping and reduced circulation, leading to increasing pericardial edema over time. Additionally, morphant embryos for either ortholog display incompletely overlapping defects in the forming vasculature of the dorsal aorta (DA), posterior caudal vein (PCV) and caudal vein (CV) which are the first blood vessels to form in the embryo. Vasculogenesis and early angiogenesis in the trunk were similarly compromised in rbm24 morphant embryos at 48 hours post fertilization (hpf). Subsequent vascular maintenance was impaired in both rbm24 morphants with substantial vessel degradation noted at 72 hpf. Conclusion: Taken collectively, our functional data support the hypothesis that rbm24a and rbm24b are key developmental cardiac genes with unequal roles in cardiovascular formation.

Original languageEnglish (US)
Article number62
JournalBMC Developmental Biology
Volume11
DOIs
StatePublished - 2011

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RNA-Binding Proteins
Embryonic Structures
Zebrafish
Fertilization
Blood Vessels
Veins
Developmental Genes
Morpholinos
Antisense Oligonucleotides
Genes
Embryonic Development
Aorta
Edema
Maintenance
Injections
RNA-Binding Motifs

ASJC Scopus subject areas

  • Developmental Biology

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Identification of RNA binding motif proteins essential for cardiovascular development. / Maragh, Samantha; Miller, Ronald A.; Bessling, Seneca L.; McGaughey, David M.; Wessels, Marja W.; De Graaf, Bianca; Stone, Eric A.; Bertoli-Avella, Aida M.; Gearhart, John D.; Fisher, Shannon; McCallion, Andrew S.

In: BMC Developmental Biology, Vol. 11, 62, 2011.

Research output: Contribution to journalArticle

Maragh, S, Miller, RA, Bessling, SL, McGaughey, DM, Wessels, MW, De Graaf, B, Stone, EA, Bertoli-Avella, AM, Gearhart, JD, Fisher, S & McCallion, AS 2011, 'Identification of RNA binding motif proteins essential for cardiovascular development', BMC Developmental Biology, vol. 11, 62. https://doi.org/10.1186/1471-213X-11-62
Maragh, Samantha ; Miller, Ronald A. ; Bessling, Seneca L. ; McGaughey, David M. ; Wessels, Marja W. ; De Graaf, Bianca ; Stone, Eric A. ; Bertoli-Avella, Aida M. ; Gearhart, John D. ; Fisher, Shannon ; McCallion, Andrew S. / Identification of RNA binding motif proteins essential for cardiovascular development. In: BMC Developmental Biology. 2011 ; Vol. 11.
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