Identification of putative c-Myc-responsive genes: Characterization of rcl, a novel growth-related gene

Brian C. Lewis, Hyunsuk Shim, Qing Li, Chyi Sun Wu, Linda A Lee, Amit Maity, Chi V. Dang

Research output: Contribution to journalArticle

Abstract

The c-Myc protein is a helix-loop-helix leucine zipper oncogenic transcription factor that participates in the regulation of cell proliferation, differentiation, and apoptosis. The biochemical function of c- Myc has been well described, yet the identities of downstream effectors are just beginning to emerge. We describe the identification of a set of c-Myc- responsive genes in the Rat1a fibroblast through the application of cDNA representational difference analysis (RDA) to cDNAs isolated from nonadherent Rat1a and Rat1a-myc cells. In this system, c-Myc overexpression is sufficient to induce the transformed phenotype of anchorage-independent growth. We identified 20 differentially expressed cDNAs, several of which represent novel cDNA sequences. We further characterized one of the novel cDNAs identified in this screen, termed rcl. rcl expression is (i) directly stimulated by c-Myc; (ii) stimulated in the in vivo growth system of regenerating rat liver, as is c-myc; and (iii) elevated in human lymphoid cells that overexpress c-myc. By using an anti-Rcl antibody, immunoblot analysis, and immunofluorescence microscopy, the Rcl protein was found to be a 23-kDa nuclear protein. Ectopic expression of the protein encoded by the rcl cDNA induces anchorage-independent growth in Rat1a fibroblasts, albeit to a diminished extent compared to ectopic c-Myc expression. These data suggest a role for rcl during cellular proliferation and c-Myc-mediated transformation.

Original languageEnglish (US)
Pages (from-to)4967-4978
Number of pages12
JournalMolecular and Cellular Biology
Volume17
Issue number9
StatePublished - Sep 1997

Fingerprint

myc Genes
Complementary DNA
Growth
Genes
Fibroblasts
Cell Proliferation
Proto-Oncogene Proteins c-myc
Leucine Zippers
Nuclear Proteins
Fluorescence Microscopy
Cell Differentiation
Anti-Idiotypic Antibodies
Proteins
Transcription Factors
Lymphocytes
Apoptosis
Phenotype
Liver

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Lewis, B. C., Shim, H., Li, Q., Wu, C. S., Lee, L. A., Maity, A., & Dang, C. V. (1997). Identification of putative c-Myc-responsive genes: Characterization of rcl, a novel growth-related gene. Molecular and Cellular Biology, 17(9), 4967-4978.

Identification of putative c-Myc-responsive genes : Characterization of rcl, a novel growth-related gene. / Lewis, Brian C.; Shim, Hyunsuk; Li, Qing; Wu, Chyi Sun; Lee, Linda A; Maity, Amit; Dang, Chi V.

In: Molecular and Cellular Biology, Vol. 17, No. 9, 09.1997, p. 4967-4978.

Research output: Contribution to journalArticle

Lewis, BC, Shim, H, Li, Q, Wu, CS, Lee, LA, Maity, A & Dang, CV 1997, 'Identification of putative c-Myc-responsive genes: Characterization of rcl, a novel growth-related gene', Molecular and Cellular Biology, vol. 17, no. 9, pp. 4967-4978.
Lewis, Brian C. ; Shim, Hyunsuk ; Li, Qing ; Wu, Chyi Sun ; Lee, Linda A ; Maity, Amit ; Dang, Chi V. / Identification of putative c-Myc-responsive genes : Characterization of rcl, a novel growth-related gene. In: Molecular and Cellular Biology. 1997 ; Vol. 17, No. 9. pp. 4967-4978.
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