TY - JOUR
T1 - Identification of prosaposin and transgelin as potential biomarkers for gallbladder cancer using quantitative proteomics
AU - Sahasrabuddhe, Nandini A.
AU - Barbhuiya, Mustafa A.
AU - Bhunia, Shushruta
AU - Subbannayya, Tejaswini
AU - Gowda, Harsha
AU - Advani, Jayshree
AU - Shrivastav, Braj R.
AU - Navani, Sanjay
AU - Leal, Pamela
AU - Roa, Juan Carlos
AU - Chaerkady, Raghothama
AU - Gupta, Sanjeev
AU - Chatterjee, Aditi
AU - Pandey, Akhilesh
AU - Tiwari, Pramod K.
N1 - Funding Information:
We thank the Department of Biotechnology (DBT), Government of India for research support to the Institute of Bioinformatics . Pramod K. Tiwari acknowledges research support from the Department of Science and Technology (DST) , Government of India and Madhya Pradesh Council of Science and Technology, Bhopal, India . Nandini A. Sahasrabuddhe is a recipient of Senior Research Fellowship from the Council for Scientific and Industrial Research (CSIR), India. Mustafa A. Barbhuiya is a recipient of Senior Research Fellowship from Indian Council of Medical Research (ICMR) , Government of India. H.C. Harsha is a Wellcome Trust/DBT India Alliance Early Career Fellow.
PY - 2014/4/18
Y1 - 2014/4/18
N2 - Gallbladder cancer is an uncommon but lethal malignancy with particularly high incidence in Chile, India, Japan and China there is a paucity of unbiased large-scale studies investigating molecular basis of gallbladder cancer. To systematically identify differentially regulated proteins in gallbladder cancer, iTRAQ-based quantitative proteomics of gallbladder cancer was carried out using Fourier transform high resolution mass spectrometry. Of the 2575 proteins identified, proteins upregulated in gallbladder cancer included several lysosomal proteins such as prosaposin, cathepsin Z and cathepsin H. Downregulated proteins included serine protease HTRA1 and transgelin, which have been reported to be downregulated in several other cancers. Novel biomarker candidates including prosaposin and transgelin were validated to be upregulated and downregulated, respectively, in gallbladder cancer using tissue microarrays. Our study provides the first large scale proteomic characterization of gallbladder cancer which will serve as a resource for future discovery of biomarkers for gallbladder cancer.
AB - Gallbladder cancer is an uncommon but lethal malignancy with particularly high incidence in Chile, India, Japan and China there is a paucity of unbiased large-scale studies investigating molecular basis of gallbladder cancer. To systematically identify differentially regulated proteins in gallbladder cancer, iTRAQ-based quantitative proteomics of gallbladder cancer was carried out using Fourier transform high resolution mass spectrometry. Of the 2575 proteins identified, proteins upregulated in gallbladder cancer included several lysosomal proteins such as prosaposin, cathepsin Z and cathepsin H. Downregulated proteins included serine protease HTRA1 and transgelin, which have been reported to be downregulated in several other cancers. Novel biomarker candidates including prosaposin and transgelin were validated to be upregulated and downregulated, respectively, in gallbladder cancer using tissue microarrays. Our study provides the first large scale proteomic characterization of gallbladder cancer which will serve as a resource for future discovery of biomarkers for gallbladder cancer.
KW - Biomarkers
KW - Gallbladder cancer
KW - Prosaposin
KW - Quantitative proteomics
KW - Transgelin
KW - iTRAQ
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U2 - 10.1016/j.bbrc.2014.03.017
DO - 10.1016/j.bbrc.2014.03.017
M3 - Article
C2 - 24657443
AN - SCOPUS:84899471783
SN - 0006-291X
VL - 446
SP - 863
EP - 869
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 4
ER -