Identification of proliferin mRNA and protein in mouse placenta

D. I H Linzer, S. J. Lee, L. Ogren, F. Talamantes, D. Nathans

Research output: Contribution to journalArticle

Abstract

Proliferin is a recently described, prolactin-related protein whose mRNA appears in several murine cell lines during active growth. We have surveyed a number of mouse organs or tissues for the presence of mRNA's that hybridize to cloned proliferin cDNA. Of the tissues tested, only the placenta yielded proliferin-related mRNA.This placental RNA is about 1 kilobase in length, increases sharply between days 8 and 10 of pregnancy, and then gradually declines through day 18. It is more abundant in RNA extracted from the fetal, compared to the maternal, part of the placenta. From a cDNA plasmid library prepared from poly(A)+ placental RNA, two types of proliferin-related clones were isolated, differing in intensity of hybridization to proliferin cDNA. By nucleotide sequence analysis, a strongly hybridizing clone was found to be nearly identical to the proliferin cDNA clone isolated from a library prepared from mRNA of a growing mouse fibroblastic cell line. Using an antiserum prepared against a synthetic proliferin fusion protein, we show that proliferin is secreted as a glycoprotein by minced placental tissue and that it differs from mouse placental lactogen. We conclude that proliferin is a placental hormone that is synthesized in certain mouse cell lines during active growth. Its function during pregnancy and during the growth of cultured cells is presently unknown.

Original languageEnglish (US)
Pages (from-to)4356-4359
Number of pages4
JournalProceedings of the National Academy of Sciences of the United States of America
Volume82
Issue number13
DOIs
StatePublished - 1985

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Placenta
Messenger RNA
Complementary DNA
Clone Cells
Proteins
Cell Line
Growth
Placental Hormones
RNA
Placental Lactogen
Pregnancy
Gene Library
Prolactin
Libraries
Sequence Analysis
Immune Sera
Cultured Cells
Glycoproteins
Plasmids
Mothers

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Identification of proliferin mRNA and protein in mouse placenta. / Linzer, D. I H; Lee, S. J.; Ogren, L.; Talamantes, F.; Nathans, D.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 82, No. 13, 1985, p. 4356-4359.

Research output: Contribution to journalArticle

Linzer, D. I H ; Lee, S. J. ; Ogren, L. ; Talamantes, F. ; Nathans, D. / Identification of proliferin mRNA and protein in mouse placenta. In: Proceedings of the National Academy of Sciences of the United States of America. 1985 ; Vol. 82, No. 13. pp. 4356-4359.
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