TY - JOUR
T1 - Identification of plasmodium GAPDH epitopes for generation of antibodies that inhibit malaria infection
AU - Cha, Sung Jae
AU - McLean, Kyle Jarrod
AU - Jacobs-Lorena, Marcelo
N1 - Funding Information:
This work was supported by grant AI123613 from the National Institute of Allergy and Infectious Diseases. We thank the Johns Hopkins Malaria Research Institute mosquito and parasite core facilities for help with mosquito rearing and with P falciparum gametocyte culture. Support from the Johns Hopkins Malaria Research Institute and the Bloomberg Philanthropies is gratefully acknowledged. Supply of human blood was supported by the National Institutes of Health grant RR00052. Molecular graphics and ana-lyses were performed with the UCSF Chimera package. Chimera is developed by the Resource for Biocomputing, Visualization, and Informatics at the University of California, San Francisco (supported by NIGMS P41-GM103311).
Funding Information:
This work was supported by grant AI123613 from the National Institute of Allergy and Infectious Diseases. We thank the Johns Hopkins Malaria Research Institute mosquito and parasite core facilities for help with mosquito rearing and with P falciparum gametocyte culture. Support from the Johns Hopkins Malaria Research Institute and the Bloomberg Philanthropies is gratefully acknowledged. Supply of human blood was supported by the National Institutes of Health grant RR00052. Molecular graphics and analyses were performed with the UCSF Chimera package. Chimera is developed by the Resource for Biocomputing, Visualization, and Informatics at the University of California, San Francisco (supported by NIGMS P41-GM103311).
Publisher Copyright:
© 2018 Cha et al.
PY - 2018
Y1 - 2018
N2 - Plasmodium sporozoite liver infection is an essential step for parasite development in its mammalian host. Previously, we used a phage display library to identify mimotope peptides that bind to Kupffer cells and competitively inhibit sporozoite–Kupffer cell interaction. These peptides led to the identification of a Kupffer cell receptor—CD68—and a Plasmodium sporozoite ligand—GAPDH—that are required for sporozoite traversal of Kupffer cells and subsequent infection of hepatocytes. Here, we report that the C-terminal end of Plasmodium GAPDH interacts with the Kupffer CD68 receptor, and identify two epitopes within this region as candidate antigens for the development of antibodies that inhibit Plasmodium infection.
AB - Plasmodium sporozoite liver infection is an essential step for parasite development in its mammalian host. Previously, we used a phage display library to identify mimotope peptides that bind to Kupffer cells and competitively inhibit sporozoite–Kupffer cell interaction. These peptides led to the identification of a Kupffer cell receptor—CD68—and a Plasmodium sporozoite ligand—GAPDH—that are required for sporozoite traversal of Kupffer cells and subsequent infection of hepatocytes. Here, we report that the C-terminal end of Plasmodium GAPDH interacts with the Kupffer CD68 receptor, and identify two epitopes within this region as candidate antigens for the development of antibodies that inhibit Plasmodium infection.
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UR - http://www.scopus.com/inward/citedby.url?scp=85057054185&partnerID=8YFLogxK
U2 - 10.26508/lsa.201800111
DO - 10.26508/lsa.201800111
M3 - Article
C2 - 30456380
AN - SCOPUS:85057054185
SN - 2575-1077
VL - 1
JO - Life science alliance
JF - Life science alliance
IS - 5
M1 - e201800111
ER -