Identification of p42 Mitogen‐Activated Protein Kinase as a Tyrosine Kinase Substrate Activated by Maximal Electroconvulsive Shock in Hippocampus

Jay M. Baraban, Rachel S. Fiore, Jasbinder S. Sanghera, Harry B. Paddon, Steven L. Pelech

Research output: Contribution to journalArticlepeer-review

Abstract

Abstract: Recent studies have demonstrated that administration of an electroconvulsive shock produces a rapid and transient increase in tyrosyl phosphorylation of a ∼40‐kDa protein in rat brain. Initial characterization of this protein's chromatographic properties indicated that it might be a member of a recently identified family of kinases, referred to as mitogen‐activated protein (MAP) kinases, that are activated by tyrosyl phosphorylation. In the present study, we have used MAP kinase antisera to assess the identity of this protein. We have found that the ∼40‐kDa phosphotyrosine‐containing protein comigrates with p42 MAP kinase (p42mapk) and not with two other 44‐kDa MAP kinase family members detected by these antisera. Western blots of proteins immunoprecipitated with MAP kinase antibodies confirm that p42mapk displays increased tyrosyl phosphorylation after an electroconvulsive stimulus. Chromatographic separation of hippocampal extracts indicates that MAP kinase activity elutes in parallel with p42mapk. Accordingly, these studies identify p42mapk as a tyrosyl kinase substrate that is activated by this stimulus and suggest that this form of MAP kinase may be selectively regulated by neuronal stimulation.

Original languageEnglish (US)
Pages (from-to)330-336
Number of pages7
JournalJournal of Neurochemistry
Volume60
Issue number1
DOIs
StatePublished - Jan 1993

Keywords

  • Extracellular signal–regulated kinases
  • Protein tyrosine phosphorylation
  • Seizures

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

Fingerprint Dive into the research topics of 'Identification of p42 Mitogen‐Activated Protein Kinase as a Tyrosine Kinase Substrate Activated by Maximal Electroconvulsive Shock in Hippocampus'. Together they form a unique fingerprint.

Cite this