Identification of OTX2 as a medulloblastoma oncogene whose product can be targeted by all-trans retinoic acid

Chunhui Di, Shaoxi Liao, David C. Adamson, Timothy J. Parrett, Daniel K. Broderick, Qun Shi, Christoph Lengauer, Jordan M. Cummins, Victor E. Velculescu, Daniel W. Fults, Roger E. McLendon, Darell D. Bigner, Hai Yan

Research output: Contribution to journalArticlepeer-review

Abstract

Through digital karyotyping of permanent medulloblastoma cell lines, we found that the homeobox gene OTX2 was amplified more than 10-fold in three cell lines. Gene expression analyses showed that OTX2 transcripts were present at high levels in 14 of 15 (93%) medulloblastomas with anaplastic histopathologic features. Knockdown of OTX2 expression by siRNAs inhibited medulloblastoma cell growth in vitro, whereas pharmacologic doses of all-trans retinoic acid repressed OTX2 expression and induced apoptosis only in medulloblastoma cell lines that expressed OTX2. These observations suggest that OTX2 is essential for the pathogenesis of anaplastic medulloblastomas and that these tumors may be amenable to therapy with all-trans-retinoic acid.

Original languageEnglish (US)
Pages (from-to)919-924
Number of pages6
JournalCancer Research
Volume65
Issue number3
StatePublished - Feb 1 2005

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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