TY - JOUR
T1 - Identification of novel short peptides derived from the α4, α5, and α6 fibrils of type IV collagen with anti-angiogenic properties
AU - Karagiannis, Emmanouil D.
AU - Popel, Aleksander S.
N1 - Funding Information:
The authors thank Zaver Bhujwalla and Roberto Pili for useful discussions; David Noren, Venu Raman, Kristine Glunde, Noriko Mori, Paul Winnard, and David Qian for their valuable advice on the experimental assays and Deborah McClellan for editorial assistance. The work was supported in part by NIH Grants NHLBI R01 HL079653 and NCI P50 CA103175.
PY - 2007/3/9
Y1 - 2007/3/9
N2 - Angiogenesis, or neovascularization, is tightly controlled by positive and negative regulators, many of which reside in the extracellular matrix. We have now identified eight novel 19- to 20-residue peptides derived from the α4, α5, and α6 fibrils of type IV collagen, which we have designated tetrastatins, pentastatins, and hexastatins, respectively. We have shown that these endogenous peptides suppress the proliferation and migration of HUVECs in vitro. By performing clustering analyses of the sequences using sequence similarity criteria and of the experimental results using a hierarchical algorithm, we report that the clusters identified by the experimental results coincide with the sequence-based clusters, indicating a tight relationship between peptide sequence and anti-angiogenic potency. These peptides may have potential as anti-angiogenic therapeutic agents.
AB - Angiogenesis, or neovascularization, is tightly controlled by positive and negative regulators, many of which reside in the extracellular matrix. We have now identified eight novel 19- to 20-residue peptides derived from the α4, α5, and α6 fibrils of type IV collagen, which we have designated tetrastatins, pentastatins, and hexastatins, respectively. We have shown that these endogenous peptides suppress the proliferation and migration of HUVECs in vitro. By performing clustering analyses of the sequences using sequence similarity criteria and of the experimental results using a hierarchical algorithm, we report that the clusters identified by the experimental results coincide with the sequence-based clusters, indicating a tight relationship between peptide sequence and anti-angiogenic potency. These peptides may have potential as anti-angiogenic therapeutic agents.
KW - Angiogenesis
KW - Endogenous
KW - Endothelial cell
KW - Hexastatin
KW - Inhibitor
KW - Pentastatin
KW - Tetrastatin
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U2 - 10.1016/j.bbrc.2006.12.231
DO - 10.1016/j.bbrc.2006.12.231
M3 - Article
C2 - 17239819
AN - SCOPUS:33846492441
SN - 0006-291X
VL - 354
SP - 434
EP - 439
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 2
ER -