Abstract
The CCAAT/enhancer binding protein β (C/EBPβ) was previously shown to bind to the α1(I) collagen promoter at -365 to -335 (site 1) and to activate it. Acetaldehyde also activates the promoter, and this effect is mediated by an increase in stellate-cell C/EBPβ protein and C/EBPβ binding. The present study identified two additional distal sites (sites 2 and 3) of binding of C/EBPβ, in the nuclear extracts of stellate cells, at -399 to -370 and -623 to -592 in the α1(I) collagen promoter. The C/EBPβ protein activates the promoter at all three sites. Acetaldehyde increases C/EBPβ binding to all three sites. Activation by acetaldehyde is abrogated in the transfected promoter mutated at either site 1 or site 3 but is not affected by mutation at site 2. Binding of the 20-kDa C/EBPβ isoform (p20C/EBPβ), which is eliminated by mutation at the distal site 3 of C/EBP binding, is necessary for the activation by acetaldehyde of the α1(I) collagen promoter.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 455-463 |
| Number of pages | 9 |
| Journal | DNA and Cell Biology |
| Volume | 20 |
| Issue number | 8 |
| DOIs | |
| State | Published - 2001 |
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ASJC Scopus subject areas
- Molecular Biology
- Genetics
- Cell Biology
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Identification of new sites of binding and activation of the murine α1(I) collagen promoter by CCAAT/enhancer binding protein β. / Attard, F. A.; Wang, L.; Potter, James John; Rennie-Tankersley, L.; Mezey, Esteban.
In: DNA and Cell Biology, Vol. 20, No. 8, 2001, p. 455-463.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Identification of new sites of binding and activation of the murine α1(I) collagen promoter by CCAAT/enhancer binding protein β
AU - Attard, F. A.
AU - Wang, L.
AU - Potter, James John
AU - Rennie-Tankersley, L.
AU - Mezey, Esteban
PY - 2001
Y1 - 2001
N2 - The CCAAT/enhancer binding protein β (C/EBPβ) was previously shown to bind to the α1(I) collagen promoter at -365 to -335 (site 1) and to activate it. Acetaldehyde also activates the promoter, and this effect is mediated by an increase in stellate-cell C/EBPβ protein and C/EBPβ binding. The present study identified two additional distal sites (sites 2 and 3) of binding of C/EBPβ, in the nuclear extracts of stellate cells, at -399 to -370 and -623 to -592 in the α1(I) collagen promoter. The C/EBPβ protein activates the promoter at all three sites. Acetaldehyde increases C/EBPβ binding to all three sites. Activation by acetaldehyde is abrogated in the transfected promoter mutated at either site 1 or site 3 but is not affected by mutation at site 2. Binding of the 20-kDa C/EBPβ isoform (p20C/EBPβ), which is eliminated by mutation at the distal site 3 of C/EBP binding, is necessary for the activation by acetaldehyde of the α1(I) collagen promoter.
AB - The CCAAT/enhancer binding protein β (C/EBPβ) was previously shown to bind to the α1(I) collagen promoter at -365 to -335 (site 1) and to activate it. Acetaldehyde also activates the promoter, and this effect is mediated by an increase in stellate-cell C/EBPβ protein and C/EBPβ binding. The present study identified two additional distal sites (sites 2 and 3) of binding of C/EBPβ, in the nuclear extracts of stellate cells, at -399 to -370 and -623 to -592 in the α1(I) collagen promoter. The C/EBPβ protein activates the promoter at all three sites. Acetaldehyde increases C/EBPβ binding to all three sites. Activation by acetaldehyde is abrogated in the transfected promoter mutated at either site 1 or site 3 but is not affected by mutation at site 2. Binding of the 20-kDa C/EBPβ isoform (p20C/EBPβ), which is eliminated by mutation at the distal site 3 of C/EBP binding, is necessary for the activation by acetaldehyde of the α1(I) collagen promoter.
UR - http://www.scopus.com/inward/record.url?scp=0034809307&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0034809307&partnerID=8YFLogxK
U2 - 10.1089/104454901316976082
DO - 10.1089/104454901316976082
M3 - Article
C2 - 11560777
AN - SCOPUS:0034809307
VL - 20
SP - 455
EP - 463
JO - DNA and Cell Biology
JF - DNA and Cell Biology
SN - 1044-5498
IS - 8
ER -