Identification of new autoimmune hepatitis-specific autoantigens by using protein microarray technology

Lin Wu, Guang Song

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Autoimmune hepatitis (AIH) is a chronic necroinflammatory disease of the liver with a poorly understood etiology. Detection of non-organ-specific and liver-related autoantibodies using immunoserological approaches has been widely used for diagnosis and prognosis. However, unambiguous and accurate detection of the disease requires the identification and characterization of disease-specific autoantigens. In the present study, we have used protein microarray technology to profile the autoantigen repertoire of patients with AIH versus those with other liver diseases, identifying and validating novel and highly specific biomarkers for AIH. Using two algorithms developed in the current study, we were able to demonstrate that a panel of six autoantigens could be used in combination to clearly diagnose AIH-positive serum samples.

Original languageEnglish (US)
Title of host publicationLiver Proteomics
Subtitle of host publicationMethods and Protocols
PublisherHumana Press Inc.
Pages227-239
Number of pages13
ISBN (Print)9781617799587
DOIs
StatePublished - Jan 1 2012
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume909
ISSN (Print)1064-3745

Keywords

  • AIH
  • Autoantibodies
  • Autoantigens
  • Autoimmune diseases
  • Protein microarray

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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  • Cite this

    Wu, L., & Song, G. (2012). Identification of new autoimmune hepatitis-specific autoantigens by using protein microarray technology. In Liver Proteomics: Methods and Protocols (pp. 227-239). (Methods in Molecular Biology; Vol. 909). Humana Press Inc.. https://doi.org/10.1007/978-1-61779-959-4_15