Identification of mycobacterium genavense in a diana monkey (cercopithecus diana) by polymerase chain reaction and high-performance liquid chromatography

Kathleen M. Kelly, Allison N. Wack, Dan Bradway, Brian W. Simons, Ellen Bronson, Gerard Osterhout, Nicole M Parrish, Richard J. Montali

Research output: Contribution to journalArticle

Abstract

A 25-yr-old Diana monkey (Cercopithecus diana) with a 1.5-yr history of chronic colitis and diarrhea was found to have disseminated granulomatous disease with intralesional acid fast bacilli. Bacilli were identified as Mycobacterium genavense by polymerase chain reaction, sequencing of the 16S-23S ribosomal RNA intergenic spacer (ITS) gene, and mycolic acid analysis by high-performance liquid chromatography. Mycobacterium genavense is a common cause of mycobacteriosis in free-ranging and captive birds. In addition, recognition of opportunistic infection in human immunodeficiency virus-positive patients is increasing. Disease manifestations of M. genavense are similar to Mycobacterium avium complex (MAC) and include fever, wasting, and diarrhea with disseminated disease. Similar clinical signs and lesions were observed in this monkey. Mycobacterium genavense should be considered as a differential for disseminated mycobacterial disease in nonhuman primates as this agent can mimic MAC and related mycobacteria.

Original languageEnglish (US)
Pages (from-to)339-344
Number of pages6
JournalJournal of Zoo and Wildlife Medicine
Volume46
Issue number2
DOIs
StatePublished - Jun 1 2015

Fingerprint

Mycobacterium genavense
Cercopithecus
Mycobacterium
Haplorhini
high performance liquid chromatography
polymerase chain reaction
High Pressure Liquid Chromatography
Mycobacterium avium complex
mycobacterial diseases
Mycobacterium avium Complex
Polymerase Chain Reaction
Bacillus
Diarrhea
diarrhea
23S Ribosomal RNA
Mycolic Acids
16S Ribosomal RNA
acids
Opportunistic Infections
colitis

Keywords

  • Diana monkey
  • mycobacteriosis
  • Mycobacterium genavense
  • mycolic acid
  • nontuberculous
  • polymerase chain reaction

ASJC Scopus subject areas

  • Animal Science and Zoology
  • veterinary(all)

Cite this

Identification of mycobacterium genavense in a diana monkey (cercopithecus diana) by polymerase chain reaction and high-performance liquid chromatography. / Kelly, Kathleen M.; Wack, Allison N.; Bradway, Dan; Simons, Brian W.; Bronson, Ellen; Osterhout, Gerard; Parrish, Nicole M; Montali, Richard J.

In: Journal of Zoo and Wildlife Medicine, Vol. 46, No. 2, 01.06.2015, p. 339-344.

Research output: Contribution to journalArticle

Kelly, Kathleen M. ; Wack, Allison N. ; Bradway, Dan ; Simons, Brian W. ; Bronson, Ellen ; Osterhout, Gerard ; Parrish, Nicole M ; Montali, Richard J. / Identification of mycobacterium genavense in a diana monkey (cercopithecus diana) by polymerase chain reaction and high-performance liquid chromatography. In: Journal of Zoo and Wildlife Medicine. 2015 ; Vol. 46, No. 2. pp. 339-344.
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