Identification of multi-copy suppressors for endoplasmic reticulum-mitochondria tethering proteins in Saccharomyces cerevisiae

Rieko Kojima, Shu Kajiura, Hiromi Sesaki, Toshiya Endo, Yasushi Tamura

Research output: Contribution to journalArticle

Abstract

In yeast, the endoplasmic reticulum (ER)-mitochondria encounter structure (ERMES) tethers the ER to mitochondria, but its primary function remains unclear. To gain insight into ERMES functions, we screened multi-copy suppressors of the growth-defective phenotype of mmm1Δ cells, which lack a core component of ERMES, and identified MCP1, MGA2, SPT23, and YGR250C (termed RIE1). Spt23 and Mga2 are homologous transcription factors known to activate transcription of the OLE1 gene, which encodes the fatty acid Δ9 desaturase. We found that Ole1 partially relieves the growth defects of ERMES-lacking cells, thus uncovering a relationship between fatty acid metabolism and ERMES functions.

Original languageEnglish (US)
JournalFEBS Letters
DOIs
StateAccepted/In press - 2016

Keywords

  • Saccharomyces cerevisiae
  • ERMES
  • Mitochondria
  • Phospholipid

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Medicine(all)
  • Molecular Biology
  • Genetics
  • Cell Biology

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