TY - JOUR
T1 - Identification of microbial DNA in human cancer
AU - Duncan, Christopher G.
AU - Leary, Rebecca J.
AU - Lin, Jimmy Cheng Ho
AU - Cummins, Jordan
AU - Di, Chunhui
AU - Schaefer, Carl F.
AU - Wang, Tian Li
AU - Riggins, Gregory J.
AU - Edwards, Jennifer
AU - Bigner, Darell
AU - Kopelovich, Levy
AU - Vogelstein, Bert
AU - Kinzler, Kenneth W.
AU - Velculescu, Victor E.
AU - Yan, Hai
N1 - Funding Information:
This work was supported by The Pediatric Brain Tumor Foundation Institute at Duke; a Damon Runyon Foundation Scholar Award; a Southeastern Brain Tumor Foundation Research Grant; an Alex's Lemonade Stand Foundation Innovation Award; a V Foundation Cancer Research Grant; NIH Grants R01CA118822, R01CA121113, NS20023-21, NS052507 and R37CA11898-34; Brain Tumor Specialized Programs of Research Excellence 5P20CA096890-02; Duke Comprehensive Cancer Center Support Grant 2P30CA14236; and grants from the Accelerate Brain Tumor Cure Foundation and the National Cancer Center, NINDS Grant 5P50 NS20023-25, NIH SPORE Grant 5P50 CA108786-4, NIH Merit Award R37 CA 011898-38, NCI Division of Cancer Prevention contract HHSN261200433002C, and The Pew Charitable Trusts.
PY - 2009
Y1 - 2009
N2 - Background. Microorganisms have been associated with many types of human diseases; however, a significant number of clinically important microbial pathogens remain to be discovered. Methods. We have developed a genome-wide approach, called Digital Karyotyping Microbe Identification (DK-MICROBE), to identify genomic DNA of bacteria and viruses in human disease tissues. This method involves the generation of an experimental DNA tag library through Digital Karyotyping (DK) followed by analysis of the tag sequences for the presence of microbial DNA content using a compiled microbial DNA virtual tag library. Results. To validate this technology and to identify pathogens that may be associated with human cancer pathogenesis, we used DK-MICROBE to determine the presence of microbial DNA in 58 human tumor samples, including brain, ovarian, and colorectal cancers. We detected DNA from Human herpesvirus 6 (HHV-6) in a DK library of a colorectal cancer liver metastasis and in normal tissue from the same patient. Conclusion. DK-MICROBE can identify previously unknown infectious agents in human tumors, and is now available for further applications for the identification of pathogen DNA in human cancer and other diseases.
AB - Background. Microorganisms have been associated with many types of human diseases; however, a significant number of clinically important microbial pathogens remain to be discovered. Methods. We have developed a genome-wide approach, called Digital Karyotyping Microbe Identification (DK-MICROBE), to identify genomic DNA of bacteria and viruses in human disease tissues. This method involves the generation of an experimental DNA tag library through Digital Karyotyping (DK) followed by analysis of the tag sequences for the presence of microbial DNA content using a compiled microbial DNA virtual tag library. Results. To validate this technology and to identify pathogens that may be associated with human cancer pathogenesis, we used DK-MICROBE to determine the presence of microbial DNA in 58 human tumor samples, including brain, ovarian, and colorectal cancers. We detected DNA from Human herpesvirus 6 (HHV-6) in a DK library of a colorectal cancer liver metastasis and in normal tissue from the same patient. Conclusion. DK-MICROBE can identify previously unknown infectious agents in human tumors, and is now available for further applications for the identification of pathogen DNA in human cancer and other diseases.
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U2 - 10.1186/1755-8794-2-22
DO - 10.1186/1755-8794-2-22
M3 - Article
C2 - 19426505
AN - SCOPUS:66149190680
SN - 1755-8794
VL - 2
JO - BMC Medical Genomics
JF - BMC Medical Genomics
M1 - 22
ER -